A light-emission detection apparatus is provided for individually condensing light emitted from each emission point of an emission-point array using each condensing lens of a condensing-lens array to forma light beam and detecting each light beam incident on a sensor in parallel. The light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

A method of analysing a sample in the form of a droplet provided on a sample-receiving surface includes providing a light source and a detector in a housing, positioning said sample-receiving surface in or on the housing, and focussing an incident beam of light to a focal point in the vicinity of the sample. Light is detected from the sample resulting from an interaction with the sample, the sample-receiving surface, or the atmosphere surrounding the sample. At least one parameter of the detected light is measured, and the sample-receiving surface is translated relative to the housing such that the focal point is at a different region of the sample, the sample-receiving surface, or the atmosphere surrounding the sample. The step of measuring one or more parameters of the detected light is repeated following the translating step.

The invention is directed to a light-emission detection apparatus for individually condensing light emitted from each emission point of a emission-point array using each condensing lens of a condensing-lens array to form a light beam and detecting each light beam incident on a sensor in parallel, and the light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Provided are an image reconstruction method, a device and a microscopic imaging device. The method includes calculating a gray value at each fiber center in a fiber bundle (04) in a reconstructed image according to a gray value at a center position of each fiber, determined in one or more sample images; performing a spatial interpolation using the gray value at the fiber center to obtain gray values of other pixel points in the fiber bundle (04) in the reconstructed image, so as to form the reconstructed image. This image reconstruction method greatly accelerates the speed of image reconstruction, and is helpful to remove the grating (022) and fiber bundle (04) cellular grid residues in the reconstructed image and improve the imaging quality of the reconstructed image.

A system for analyzing a sample includes an illumination source with a plurality of transmitting optical fibers optically coupled to the illumination source and a detector with a plurality of receiving optical fibers optically coupled to the detector. The system further includes a plurality of probes coupled to respective ones of the plurality of transmitting optical fibers and respective ones of the plurality of receiving optical fibers. The plurality of probes are configured to illuminate respective portions of a surface of the sample and configured to receive illumination reflected, refracted, or radiated from the respective portions of the surface of the sample. The system may further include one or more switches and/or splitters configured to optically couple respective ones of the plurality of transmitting optical fibers to the illumination source and/or configured to optically couple respective ones of the plurality of receiving optical fibers to the detector.

A detection device, a detection apparatus, and a detection method are provided. The detection device includes a detection assembly and a driving assembly. At least two rows of detection units are provided, and the detection units are arranged in a matrix along the first direction and the second direction. During detection, the driving assembly may drive one or both of the to-be-detected object and the detection assembly to move along at least one of the first direction and the second direction, so that the detection units scan different areas of the surface of the to-be-detected object, thereby realizing a scanning of the entire surface. That is, the scanning of the surface of the to-be-detected object is completed by using multiple detection units, which improves detection efficiency for the to-be-detected surface, and realizes a relatively high imaging quality.

Near-Infrared (NIR) filter photometry is used to calculate component concentrations in multiphase flows. The disclosed methodology adapts the Beer-Lambert law for nonhomogeneous immiscible mixtures (such as oil and water) by modeling the fluid layer as a nonhomogeneous distribution of its components and deriving a mathematical relationship between measured absorbances, component path lengths, and non-homogeneity factors. The methodology is integrated into a multi-channel filter photometer to measure phase concentrations in oil-and-gas pipelines. The system is proven more accurate than current state of the art based on data from simulations, multiphase flow laboratories and field trials.

In a reaction or growth monitoring system, the temperature of a reaction vessel is controlled using heat from a semiconductor sensor placed in direct or thermal contact with the reaction vessel. The heat from the semiconductor sensor is controlled by monitoring the temperature at the reaction vessel and by controlling accordingly, the operation of the sensor and/or by controlling a cooling mechanism in thermal contact with the semiconductor sensor. Additional heat may be provided to the reaction vessel via electromagnetic radiation from an electromagnetic illumination source.

A biological sample analysis system including a sample preparation system forming droplets of segmented sample separated by a carrier fluid immiscible with the sample. The droplets include reaction mixtures for amplification of at least one target nucleic acid. A thermal cycling device having a sample block having a plurality of controlled thermal zones, and a containment structure in thermal communication with the plurality of controlled thermal zones. The containment structure receives and contains the droplets of segmented sample separated by the immiscible carrier fluid from the sample preparation system. A controller for controlling a temperature in each thermal zone of the sample block. A detection system detects electromagnetic radiation emitted from each of the droplets individually from the queue of droplets as they flow past the detection system. A positioning system to facilitate moving a queue of the droplets in the thermal cycling device relative to the detection system.

A spectrometer system and method including a laser source for directing a laser beam to a sample producing plasma radiation on the sample. At least one fiber of a fiber bundle is connected to an illumination source for directing light to the sample. A spectrometer subsystem receives plasma radiation from the sample via the detection fiber bundle. A camera receives light from the illumination source reflected off the sample for detecting the presence of the sample.