In one aspect, the present invention relates to a system evaluating a surgical margin of tumor tissues of a living subject. In one embodiment, the system includes a light source configured to emit a source light; at least one optical probe; a scanner; a spectrometer; and a controller coupled with the scanner and the spectrometer for operably controlling the scanner and the spectrometer. In operation, a working end of the optical probe is positioned proximate to a surface of a specimen of the tumor tissues. A source channel of the optical probe deliver the source light emitted by the light source from the working end to the surface of the specimen, and a plurality of collection channels collect from the working end diffused/reflected light generated from interaction of the source light with the specimen. The spectrometer receives the collected diffused/reflected light to evaluate a margin status of the specimen.

The present invention provides a multichannel label-free biosensing fiber-optic system, which comprises one or more light sources coupled into optical fibers, one or more optical fiber circuits for performing coupling or/and directional transmission of optical-fiber guided lightwaves, one or more optical-fiber-input and optical-fiber-output optical switches, a plurality of optical fibers provided with label-free optical sensing elements working in the reflection manner on the optical fiber ends, and the light detection parts, wherein the optical-fiber-input and optical-fiber-output optical switch is provided with a plurality of outputs and/or a plurality of inputs, and with the plurality of outputs and/or plurality of inputs, by the switching function, the reflected light from the label-free optical sensing elements working in the reflection manner on the designated optical fiber ends is received by the light detection part, so that multichannel sensing is realized.

Systems and methods here may be used for capturing and analyzing spectrometer data of multiple sample gemstones on a stage, including mapping digital camera image data of samples, for both reflective and transmission modes.

A photoacoustic remote sensing system (PARS) for imaging a subsurface structure in a sample has an excitation beam configured to generate ultrasonic signals in the sample at an excitation location; an interrogation beam incident on the sample at the excitation location, a portion of the interrogation beam returning from the sample that is indicative of the generated ultrasonic signals; an optical system that focuses at least one of the excitation beam and the interrogation beam with a focal point that is below the surface of the sample; and a detector that detects the returning portion of the interrogation beam.

The present invention provides a tapered side-polished fiber-optic biosensor (FOBS) and a method for preparing a tapered side-polished fiber (SPF). The biosensor includes a broadband light source, a first single-mode fiber, a tapered SPF, a second single-mode fiber, and a spectrometer. The broadband light source is connected to the tapered SPF through the first single-mode fiber, and the tapered SPF is connected to the spectrometer through the second single-mode fiber. The broadband light source is configured to emit a light wave. The spectrometer is configured to display a spectrum corresponding to a light wave passing through the first single-mode fiber, the tapered SPF, and the second single-mode fiber successively. In the present invention, a fiber side-polishing technology is combined with a fiber tapering technology to construct a tapered SPF, and a spectrum changes by changing a refractive index around a side-polished tapered region, thereby measuring the refractive index. In addition, the tapered SPF provided in the present invention can generate a Vernier effect, thereby improving the sensor's anti-electromagnetic interference and sensitivity to refractive index measurement.

A spectroscopic system may include: a probe having a probe tip and an optical coupler, the optical coupler including an emitting fiber group and first and second receiving fiber groups, each fiber group having a first end and a second end, wherein the first ends of the fiber groups are formed into a bundle and optically exposed through the probe tip; a light source optically coupled to the second end of the emitting fiber group, the light source emitting light in at least a first waveband and a second waveband, the second waveband being different from the first waveband; a first spectrometer optically coupled to the second end of the first receiving fiber group and configured to process light in the first waveband; and a second spectrometer optically coupled to the second end of the second receiving fiber group and configured to process light in the second waveband.

An optical fiber splitter device comprising at least two optical fibers of different lengths is disclosed for partial or complete compensation of the optical path difference between waves interfering to generate a hologram or an interferogram. Various implementations of this fiber splitter device are described in apparatuses for holographic and interferometric imaging of microscopic and larger samples.

An apparatus or method for measurement of multiple gas concentrations. The apparatus comprises a multi-wavelength laser module, and a multi-layered/multi-band detector.

Provided are methods and devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises flow and hydrodynamic, electrokinetic, and optical forces for the analysis and sorting of samples, wherein the samples comprise liquid or particles in microfluidic channels, and wherein the devices comprise an assembly of components that enable processing of a said samples for analytical assessment by fluidic and/or particle based instruments. Microfluidic structures (channels, “T's”, “Y's”, branched “Y's”, wells, and weirs) are described for facilitating sample interaction and observation, sample analysis, sorting, or isolation. Detection can be accomplished using spectroscopic methods including, but not limited to, Raman spectroscopy of single cells and bulk cellular samples (collections of cells; several individuals to hundreds or thousands of cells).

An apparatus for spectral analysis comprises a first lens holder comprising a first lens, a second lens holder comprising a second lens, a first optical fiber, and a spectrometer. The first lens and the second lens are for receiving a scattered light beam and focusing it to a point. The first optical fiber is arranged between the first lens holder and the second lens holder. The first optical fiber receives a first light beam focused by the first lens, transmits the first light beam through, and then projects the first light beam on the second lens. The spectrometer is positioned on a side of the second lens holder opposite the first optical fiber. The second lens focuses the first light beam received from the first optical fiber and projects the first light beam onto the spectrometer so that the spectrometer can analyze the first light beam.