Methods and systems for optimal capture of a multi-channel image in a LSPR spectrometry is described herein. The method comprises 1) finding a plurality of valid groupings of channels, 2) determining total capture times for each valid grouping of channels, 3) determining a measure of exposure sub-optimality, 4) estimating the expected error in peak wavelength for each valid grouping, 5) finding an optimal grouping of channels by identifying the grouping with the lowest estimate of expected error in peak wavelength and 6) capturing a subsequent multi-channel image using the optimal grouping.

The present invention relates to a method for evaluating coagulability of a blood specimen obtained from a subject to whom a substance having a coagulation factor VIII-substituting activity is administered. The present invention also relates to a reagent for blood coagulation analysis, a reagent kit for blood coagulation analysis, and an apparatus for blood coagulation analysis. Furthermore, the present invention relates to an apparatus and computer program for evaluating coagulability of a blood specimen.

An information processing device including a processor that acquires an operating parameter regarding a reaction device that performs a predetermined chemical reaction and information regarding a substance used in the chemical reaction in the reaction device, the information processing device determines a parameter of a first reaction state predicted as the reaction state of the substance when the chemical reaction is performed with the operating parameter in the reaction device, the information processing device learns a prediction model that outputs a parameter of a second reaction state that is a reaction state of the substance in response to inputting information regarding the substance and the operating parameter by using the acquired information regarding the substance, the acquired operating parameter and the parameter of the first reaction state as learning data, and the information processing device stores the learned prediction model in a storage unit.

Provided herein are methods, systems and digital microfluidic cartridges for the determination of ligand-target analyte affinity value (K.sub.D), determination of an association rate constant (k.sub.on), and/or determination of a dissociation rate constant (k.sub.off) in a PR (plasmon resonance) based system. More specifically, as described herein, the present disclosure is directed to improved methods, systems and digital microfluidic cartridges for determining a dissociation rate constant (k.sub.off) using free ligand molecules in the dissociation phase or dissociation step of a standard kinetic binding curve or in ligand-target analyte affinity analysis (referred to herein as ligand assisted dissociation (LAD)).

Provided herein are systems for the label-free detection of target molecules in samples. The systems include a sensor probe positioned in a sensing region and configured to bind to receptors for the target molecules. The systems also include electrodes configured to expose the sensor probe to an alternating electric field, and a light source optically coupled to the sensor probe and configured to provide light along a length of the sensor probe. In addition, the systems also include a position sensitive photodetector configured to detect a position of light exiting the sensor probe, and a processor configured to assess, based at least in part on the position of the light exiting the sensor probe, an amplitude of oscillation of the sensor at a frequency of the alternating electric field and a direction of a displacement of the sensor. Additional systems and related methods are also provided.

A method and system for analysis of protein interaction kinetics in microarray or whole-cell based formats includes positioning a sensor chip on a prism. The sensor chip is spotted with a plurality of target molecules. A movable printer head deposits a plurality of analyte droplets on predefined regions of the sensor chip surface. A light source transmits light through the prism to excite surface plasmon resonance on the sensor chip surface, whereby the plurality of target molecules bound to the upper surface are changing the SPR resonance angle and therefore the intensity of the reflected beam. A detector receives reflected light transmitted through the prism from the bottom surface. Signals from the detector are received and processed into kinetic data and microarray labeled data to determine molecular interactions and binding kinetic properties for the plurality of analyte droplets.

Provided herein are systems for the label-free detection of target molecules in samples. The systems include a sensor probe positioned in a sensing region and configured to bind to receptors for the target molecules. The systems also include electrodes configured to expose the sensor probe to an alternating electric field. and a light source optically coupled to the sensor probe and configured to provide light along a length of the sensor probe. In addition, the systems also include a position sensitive photodetector configured to detect a position of light exiting the sensor probe, and a processor configured to assess. based at least in part on the position of the light exiting the sensor probe. an amplitude of oscillation of the sensor at a frequency of the alternating electric field and a direction of a displacement of the sensor. Additional systems and related methods are also provided.

An information processing device including a processor that acquires an operating parameter regarding a reaction device that performs a predetermined chemical reaction and information regarding a substance used in the chemical reaction in the reaction device, the information processing device determines a parameter of a first reaction state predicted as the reaction state of the substance when the chemical reaction is performed with the operating parameter in the reaction device, the information processing device learns a prediction model that outputs a parameter of a second reaction state that is a reaction state of the substance in response to inputting information regarding the substance and the operating parameter by using the acquired information regarding the substance, the acquired operating parameter and the parameter of the first reaction state as learning data, and the information processing device stores the learned prediction model in a storage unit.

Provided herein are methods of performing multiplex detection of ligand binding kinetics. In some embodiments, the methods include contacting ligands with an array of nucleic acid barcoded oscillators disposed on a first surface of a substrate that comprises an electrically conductive coating, applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of ligand binding data. In some embodiments, the methods also include contacting barcode decoding nucleic acids with the array of nucleic acid barcoded oscillators applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of barcode decoding data.

According to one embodiment, an immunoassay system includes a measurement unit, a calculation unit, and a selection unit. The measurement unit measures a measurement target substance contained in a specimen in accordance with a measurement sequence, and acquires a measurement signal reflecting a concentration of the measurement target substance. The calculation unit calculates an index value related to a fluctuation in intensity of the measurement signal during a first period. The selection unit selects a single measurement sequence to be used in processing during or after the first period in accordance with a concentration range corresponding to an index value of the measurement target substance.