A signal acquisition device includes: a light source that oscillates pulsed laser light at a specific repetition period; an optical system that focuses the laser light onto a sample, and that collects generated fluorescence; a photodetector that detects the fluorescence collected by the optical system; an A/D converter that samples an intensity signal of the detected fluorescence, in synchronization with the repetition period of the light source unit, at a period that is an integer multiple of the repetition period, and that generates a digital intensity signal; and one or more processors comprising hardware, the one or more processors being configured to: obtain a fluorescence lifetime waveform on a basis of the generated digital intensity signal; and calculate a fluorescence lifetime coefficient from a waveform obtained by removing a region not corresponding to an exponential function from the obtained fluorescence lifetime waveform.

A method of reading a coupon channel that displays a test section pattern after being exposed to a target substance, the method uses a device having a computer readable memory, digital camera, logic assembly and user interface; providing a pixel target intensity profile; placing the coupon in the device and exposing the coupon channel to a test fluid mixture; automatically using the digital camera to take a digital image of the coupon channel test section after the exposure. The improvement in the method includes finding the contiguous set of pixels from the test section of the coupon channel that best matches the intensity profile of the target pattern representation and determining if this best match set of pixels exceeds a similarity threshold and in response to a best match set of pixels passing the similarity threshold, automatically providing a human perceptible indication that the target substance has been detected.

The prism-coupling systems and methods include using a prism-coupling system to collect a 2D digital mode spectrum of an IOX article. The mode line and critical angle positions and orientations are found by performing a weighted fit to mode line and critical angle images and are used to define a compensated mode spectrum. If mode line tilt is found, it is removed from the 2D digital mode spectrum to define the compensated mode spectrum. The compensated mode spectrum is then processed using techniques known in the art to provide a more accurate estimate of stress-related characteristics of the IOX sample versus using the uncompensated mode spectrum. Derivative-based methods of accurately establishing positions of intensity transitions in a mode spectrum of an IOX sample using a derivative spectrum and curve fitting are also disclosed.

Aspects of the present disclosure include reconfigurable integrated circuits for characterizing particles of a sample in a flow stream. Reconfigurable integrated circuits according to certain embodiments are programmed to calculate parameters of a particle in a flow stream from detected light; compare the calculated parameters of the particle with parameters of one or more particle classifications; classify the particle based on the comparison between the parameters of the particle classifications and the calculated parameters of the particle; and adjust one or more parameters of the particle classifications based on the calculated parameters of the particle. Methods for characterizing particles in a flow stream with the subject integrated circuits are also described. Systems and integrated circuit devices programmed for practicing the subject methods, such as on a flow cytometer, are also provided.

An embodiment provides a method for measuring sulfite in a solution, including: preparing a hemicyanine indicator; introducing the hemicyanine indicator to a solution containing an amount of sulfite, wherein the hemicyanine indicator reacts with the sulfite and causes a change in fluorescence of the solution; and measuring the amount of sulfite in the solution by measuring an intensity of the fluorescence. Other aspects are described and claimed.

An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

A universal rapid diagnostics test reader is disclosed and described herein that includes a set of control electronics, a digital camera component, an illumination component, a housing component, and a rapid diagnostics test tray, wherein the tray can hold at least one rapid diagnostics test having a shape and a size in a fixed position relative to the digital camera component and the illumination component, and wherein the reader can accommodate more than one different rapid diagnostics test. Methods are also disclosed that include: providing at least one first rapid diagnostics test having a first physical size, first feature and first format; providing at least one second rapid diagnostics test having a second physical size, second feature and second format; inserting the first rapid diagnostics test in a universal rapid diagnostics test reader; analyzing the first rapid diagnostics test using the universal rapid diagnostics test reader; removing the first rapid diagnostics test from the reader; inserting the second rapid diagnostics test in a universal rapid diagnostics test reader without any mechanical adjustments of the reader or without the use of any additional parts or additional inserts; and analyzing the second rapid diagnostics test using the universal rapid diagnostics test reader.

A molten metal inclusion test apparatus includes a spectroscopic appliance for gathering data indicative of the contents of a quantity of molten metal. Laser induced emissions provide spectral data based on the elements present in the melt. Analysis of a series of samplings, or shots of laser induced emissions indicates a presence of elements above a background or expected level. These elements appear as spikes in a graphical rendering of the spectral data, defined by a wavelength of the detected element. Correlation of the elements detected in the same shot indicates a composition of the inclusion, typically a particle of an extraneous compound in the melt. Such spectral analysis provides immediate feedback about melt quality, allowing corrective measures to be taken prior to casting.

An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.