An apparatus for determining blood clotting capacity comprises an actuator to cyclically move a member within a sample of blood received in a well in a tray and one of a deflection sensor and a position sensor to determine the position of the wetted member upon being acted upon by the actuator. The theoretical position of the wetted member, as determined using a known actuator force and wetted member physical data, is compared to the sensed deflection or position of the wetted member, and the resistance to movement of the wetted member caused by the blood is determined and correlated to a clotting capacity.

A system and method is provided for measuring a mechanical property of a biological tube. The system and method operate to arrange a plurality of piezoelectric elements about the biological tube and apply a predetermined force or transduce an endogeneous or exogeneous force to the biological tube. The system and method also operate to receive a respective signal from each piezoelectric element in the plurality of piezoelectric elements responsive to the application of the predetermined force or a transduced endogenous or exogeneous force and calculate the mechanical property of the biological tube based on the signals received from the plurality of piezoelectric elements.

The present grip design includes a two-piece clamp with an interior space which forms a mold for the sample material. The two-piece clamp further includes undercut apertures which engage complementary tapered portions of upper and lower grips. The sample material can be poured to fill the mold formed within the two-piece clamp. The interior of the upper and lower grips includes a pattern, such as, but not limited to, a threaded pattern, in order to more firmly engage the sample. The samples may include soft materials, liquids, gels, compounds, powdered or similar materials. The grip may be used in connection with bioreactor or materials testing applications.

A system for measuring cellular forces exerted on a surface comprising: a deformable sensor that has a deformable surface adapted to deform on application of a cellular force, and an optical detector for optically detecting deformation of the surface.

A device and method for the non-invasive measurement of state of tension, biomechanical and viscoelastic properties of surfaces of soft biological tissues includes electronic, digital and mechanical elements and sensors, and a testing end. The elements within the housing can subject the testing end to a mechanical impulse force, and can sense the movement of the testing end. The testing end has a contact surface which can be adhered to the surface of the soft biological tissue, and subjected to a mechanical force in a direction parallel to the surface.

An assay for measuring cell-generated forces, is disclosed. The assay includes a first substrate comprising an elastic material having a Young's modulus of between about 0.1 kPa to about 50 kPa and a geometric micropattern disposed onto a surface of the substrate. The micropattern is further comprised of at least one segment consisting of a fluorophore-conjugated material, a material with cell adhesion properties, and has an interior region.

A low-cost, magnetically-driven device that enables visualization and quantification of dynamic changes in cell behavior during mechanical stretch. Using this device, it was observed that nuclei of mouse embryonic skin fibroblasts underwent rapid but divergent responses to strain magnitude, showing nuclear area increase and chromatin decompaction during 5% (low) strain, but nuclear area decrease and chromatin condensation during 20% (high) strain. Only responses to low strain were dependent on calcium, while actin inhibition abrogated any nuclear response and increased stretch-induced DNA damage. Stretch-activation revealed a shift in actin filaments away from (low strain) or towards (high strain) the nuclear periphery. The findings suggest that different pathways control strain level-dependent cell behavior and that mechanical confinement of nuclei through actin may be a protective mechanism during high strain loads.

The present invention provides methods for determining malignancy in a cyst by measuring viscosity of cyst fluid while under stain. The invention further provides devices, and computer program products for determining same.

A system for measuring cellular forces exerted on a surface comprising: a deformable sensor that has a deformable surface adapted to deform on application of a cellular force, and an optical detector for optically detecting deformation of the surface.

Systems and methods for measuring the properties (e.g., mechanical properties) of particles such as biological entities, in a fluidic channel(s) are generally provided. In some embodiments, the systems and methods comprise measuring an acoustic scattering of single particles. For example, a single particle (e.g., a biological entity) may be flowed in a suspended fluidic channel (e.g., a suspended microfluidic channel) and the fluidic channel is oscillated at or near a (mechanical) resonant frequency (e.g., at a second or higher bending mode) of the suspended fluidic channel. In some cases, an acoustic scattering signal (e.g., the change in resonant frequency of the fluidic channel as the particle flows along a longitudinal axis of the channel) may correspond to a property (e.g., a mechanical property, a cross-linking density, a transport rate of small molecules into/out of the particle) of the particle. In certain embodiments, the systems and methods comprise determining a node deviation due to a single particle (or node deviations for a plurality of particles).