Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (M.sub.c), a reporter molecule and two or more lipophilic groups (G.sub.L) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.

Described herein are examples of industrial radiography systems that enable rotation of a part about a custom axis that is offset from an actual rotation axis of a rotatable fixture that retains the part. This may be valuable in situations where it is difficult, impractical, and/or impossible to align the center of the part with the center of the rotatable fixture. In some examples, the custom axis rotation may be implemented on existing radiography machines, without requiring physical alteration of the radiography machines, integration of new components into the radiography machines, and/or risk of instability to the part and/or radiography machines.

A processor is configured to: register the fluoroscopy apparatus of which the processor controls the fluoroscopy and another fluoroscopy apparatus other than the fluoroscopy apparatus in association with each other; notify the other fluoroscopy apparatus of an imaging condition with the pulsed radiation in a case where an instruction to start emitting radiation is given in the fluoroscopy apparatus of which the imaging control device controls the fluoroscopy; and control, in a case where the imaging condition is notified from the other fluoroscopy apparatus in a case where the instruction to start emitting the radiation is not given in the fluoroscopy apparatus of which the imaging control device controls the fluoroscopy, and the instruction to start emitting the radiation is given, the fluoroscopy to emit the pulsed radiation, at timing at which the emission of the pulsed radiation is stopped in the other fluoroscopy apparatus based on the imaging condition.

The presently-disclosed subject matter includes fluorescent compounds of the following formula: ##STR00001##
The compounds can be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

In a fluoroscopic image capturing apparatus, an internal structure of a specimen can be appropriately evaluated. To provide a fluoroscopic image capturing apparatus 1 including: a timing control device 13 configured to output an irradiation timing signal S1 in synchronization with a drive timing signal S4 for driving a specimen 31 or a timing signal S5 as a detection result of an operation of the specimen 31; electromagnetic wave generation units 22 and 23 configured to irradiate, in synchronization with the irradiation timing signal Si, the specimen 31 with a pulsed electromagnetic wave beam B1 having a wavelength with which the electromagnetic wave beam B1 is transmitted through the specimen 31; and an electromagnetic wave detection device 41 configured to receive the electromagnetic wave beam B1 transmitted through the specimen 31.

A system applies Bayesian inference techniques to determine parameters of a camera and an illumination profile for an inhomogeneously-illuminated sample from observation data. The system learns full posterior probability distributions over all parameters involved in the imaging process that works in both high illumination and low illumination regimes.