Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-home pathogens are disclosed.

An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C. hyointestinalis and polynucleotides encoding it, and novel methods for detection of C. hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni, C. coli, and C. fetus, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified as C. hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

The present invention provides methods for diagnosing and treating Helicobacter pylori infection by using a monoclonal antibody to detect Helicobacter pylori neutrophil-activating protein (HP-NAP) and inhibit the activity thereof. The monoclonal antibody is an anti-FLAG antibody that binds to a specific epitope on HP-NAP for the detection of HP-NAP in its native form or denatured form. Furthermore, the present invention uses the anti-FLAG antibody to block HP-NAP-induced production of reactive oxygen species by human neutrophils. Thus, the present invention can be applied in clinical diagnosis of Helicobacter pylori infection as well as treatment of the infection via inhibiting Helicobacter pylori-induced inflammation.

Provided are a method and a reagent for measuring an antibody against Helicobacter suis using an outer membrane fraction of H. suis. A reagent for detecting an antibody that binds to a H. suis outer membrane fraction, or a protein contained in the outer membrane fraction, of a specimen derived from a subject, the reagent comprising at least the H. suis outer membrane fraction, or the protein contained in the outer membrane fraction, and an anti-Ig antibody.

Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

The present invention relates to a method of detecting a microorganism having hippuricase activity comprising contacting a sample to be tested with p-hydroxyhippuric acid or a salt thereof, a detection reagent and an oxidizing reagent. The present invention further relates to a kit comprising p-hydroxyhippuric acid, a detection reagent and an oxidizing reagent, and further relates to the use of the kit for detecting a microorganism having hippuricase activity or for discriminating a microorganism having hippuricase activity from a microorganism without hippuricase activity or to the use of the kit in a method of detecting a microorganism having hippuricase activity.

The present invention is directed to isolated bacteriophages having specificity and lytic activity against strains of Campylobacter species, methods of using the bacteriophages, progeny and derivatives derived therefrom, to control the growth of Campylobacter species in various settings (e.g., food safety, sanitation, modulating microbiome, prebiotics, probiotics).

There is provided novel peptides for use in diagnosis of CagA+ H. pylori infection or the prediction of risk for gastric cancer. The peptides bind antibodies from CagA+ H pylori patients with high specificity and sensitivity, and can be used for example in a diagnostic kit.

Present invention relates to the in vitro use of the level or concentration in a salivary or breath sample of bacteria belonging to the Alloprevotella, Prevotella, Campylobacter, Rothia, Catonella, Porphyromona, Fretibacterium genus, or any combination thereof, for the diagnosis of carcinomas or epidermoid cancers, especially epidermoid cancer of the head and neck, in a patient, or to obtain useful data that allow such a diagnosis.