The invention relates to a method for determining the presence of an analyte by means of a distribution of small magnetic particles. According to said method, the magnetizations of the small particles are oriented in relation to each other by means of an outer magnetic focusing field; once the focussing field has been terminated, the magnetizations of the small particles are rotated asynchronously to the magnetic field by means of an outer magnetic field of suitable field intensity and rotational frequency, which rotates about a longitudinal axis (z); the temporal course of the superpositioned transverse magnetization of the set of particles is detected; and the presence of the analyte is deduced from the detected temporal course. The invention also relates to a corresponding device (1).

A device for magnetically detecting microscopic biological objects includes a microfluidic channel having a fluid inlet and a fluid outlet; a plurality of magnetic sensors arranged against an inner wall of the microfluidic channel; and a permanent magnet arranged against an outer wall of the microfluidic channel, in such a way that the plurality of magnetic sensors is immersed in a magnetic field created by the permanent magnet; the plurality of magnetic sensors including a first magnetic sensor; a second magnetic sensor opposite the first magnetic sensor; a third magnetic sensor downstream of the first and second magnetic sensors.

A system for detecting analytes in a test sample, and a method for processing the same, is provided. The system includes a cartridge reader unit that has a control unit and a pneumatic system, and a cartridge assembly that prepares the samples with mixing material(s) through communication channels. The assembly has a memory chip with parameters for preparing the sample and at least one sensor. The assembly, pneumatic system, and control unit operate together to prepare the sample and provide the prepared sample to the sensor for detecting analytes, and also process measurements from the sensor to generate test results.

The system disclosed comprises an apparatus and a method for the detection and quantification of targeted molecules in vivo. The apparatus comprises an implantable biosensor and an AC magnetic detection device. The implantable biosensor includes functionalized nanoparticles functionalized with one or more moieties that bind to a molecular target of interest. The nanoparticles are retained in a biocompatible container which allows the molecular target of interest to enter the biosensor, for example through a semipermeable port. The biosensor can be implanted minimally-invasively into humans or animals. Upon exposure of the nanoparticles to the molecular target, a change in Neel relaxation time can be externally detected and correlated to the target analytes concentration. The change in relaxation time is detected through magnetic AC spectrometric measurements at a frequency specifically tuned to the nanoparticle type of interest Additionally, a method is provided for quantifying one or several biosensors within one specimen.

A method and test system for detecting the presence of an analyte in a sample comprising a binding region and a detecting region, where the binding region contains a plurality of magnetic beads attached to a plurality of first capture molecules that bind to an analyte of interest in the sample, and a plurality of second capture molecules having a detectable label attached thereto, where the second capture molecules bind to the analyte of interest to form a complex, where the complexes are moved through at least one liquefied aliphatic partition via a magnetic field into the detecting region that having a detection composition allows for detection and, optionally, for signal quantification.

Described is an apparatus comprising magnetisable particles adapted for binding to an analyte, the apparatus comprising a sensing zone comprising least an array of magnetic field sensors, a sample introduction device configured to introduce the sample to the sensing zone, optionally a field generator (optimised for magnetic and/or electric field generation) if the magnetisable particles do not have an aligned dipole moment, a controller connected to receive signals from the array of magnetic and/or electric field, the controller configured to determine an amount of analyte in the sample based on the signals received from the array of magnetic and/or electric field sensors, and an additional feature selected from one or more of a set and reset module or capability for performing a set/reset of the magnetic sensors, a data transmission layer, that is configured to shield the signals being transmitted from the one or more magnetic sensors, a plurality of magnetic field transmission zones corresponding to an area below each magnetic sensor, and a printed circuit board comprising one or more vias connecting to the magnetic field sensors.

Disclosed herein are detection methods that use magnetic nanoparticles (MNPs) to allow molecules to be identified. Embodiments of this disclosure include methods of using magnetic sensors (e.g., magnetoresistive sensors) to detect temperature-dependent magnetic fields (or changes in magnetic fields) emitted by MNPs, and, specifically to distinguish between the presence and absence of magnetic fields emitted, or not emitted, by MNPs at different temperatures selected to take advantage of knowledge of how the MNPs' magnetic properties change with temperature. Embodiments disclosed herein may be used for nucleic acid sequencing, such as deoxyribonucleic acid (DNA) sequencing.

A system and method for locating magnetic material. In one embodiment the system includes a magnetic probe; a power module in electrical communication with the magnetic probe to supply current to the magnetic probe; a sense module in electrical communication with the magnetic probe to receive signals from the magnetic probe; and a computer in electrical communication with the power module and the sense module. The computer generates a waveform that controls the supply of current from the power module and receives a signal from the sense module that indicates the presence of magnetic material. The magnetic probe is constructed from a material having a coefficient of thermal expansion of substantially 10.sup.6/ C. or less and a Young's modulus of substantially 50 GPa or greater. In one embodiment magnetic nanoparticles are injected into a breast and the lymph nodes collecting the particles are detected with the probe and deemed sentinel nodes.

In one aspect, a magnetic-field biosensor includes an insulator and a plurality of magnetic-field sensing elements that includes a first and a second magnetic-field sensing elements. The insulator has a first and a second plurality of portions, and the second plurality of portions is thicker than the first plurality of portions. The magnetic-field biosensor further includes a first receptor configured to attach to biological material and being on a first portion of the first plurality of portions and directly above the first magnetic-field sensing element; and a second receptor configured to attach to the biological material and being on a first portion of the second plurality of portions and directly above the second magnetic-field sensing element. Outputs of the first and the second magnetic-field sensing elements are used to sense a magnetic field from a first magnetic nanoparticle by reducing an effect of an applied magnetic field.

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.