The invention relates to a method for the hyperpolarization of a material sample (4), which hits a number of first spin moments (10) of a first spin moment type, wherein the number of first spin moments (10) is brought into interaction with a second spin moment (16) of a second spin moment type, wherein the first spin moments (10) are nuclear spin moments and the second spin moment (16) is an election spin moment, wherein the first and second spin moments (10, 16) are exposed to a homogeneous magnetic field (B), wherein the second spin moment (16) is polarized along the magnetic field (B), wherein the second spin moment (16) is coherently manipulated by means of a, preferably repeated, sequence (S) having a number of successive high-frequency pulses (P.sub.ki, P.sub.ki) temporally offset to each by durations (T.sub.ki, T.sub.ki, T), in such a way that a polarization transfer from the second spin moment (16) to the first spin moments (10) occurs, and wherein durations (T.sub.ki, T.sub.ki, T) inversely proportional to a Lamor frequency (.sub.Larmor) of the first spin moments (10) in the magnetic field (B) are inserted between high-frequency pulses (P.sub.ki, P.sub.ki).

The invention relates to a method for the hyperpolarization of a material sample (4), which hits a number of first spin moments (10) of a first spin moment type, wherein the number of first spin moments (10) is brought into interaction with a second spin moment (16) of a second spin moment type, wherein the first spin moments (10) are nuclear spin moments and the second spin moment (16) is an election spin moment, wherein the first and second spin moments (10, 16) are exposed to a homogeneous magnetic field (B), wherein the second spin moment (16) is polarized along the magnetic field (B), wherein the second spin moment (16) is coherently manipulated by means of a, preferably repeated, sequence (S) having a number of successive high-frequency pulses (P.sub.ki, P.sub.ki) temporally offset to each by durations (T.sub.ki, T.sub.ki, T), in such a way that a polarization transfer from the second spin moment (16) to the first spin moments (10) occurs, and wherein durations (T.sub.ki, T.sub.ki, T) inversely proportional to a Lamor frequency (.sub.Larmor) of the first spin moments (10) in the magnetic field (B) are inserted between high-frequency pulses (P.sub.ki, P.sub.ki).

A method for separating oil-water two-phase NMR signals by using dynamic nuclear polarization comprising: using a combination of a non-selective free radical and a selective relaxation reagent to selectively enhance an NMR signal of an oil phase or a water phase, the relaxation reagent being capable of selectively suppressing dynamic polarization enhancement of the water phase or oil phase, thus achieving the polarization enhancement of a single fluid phase in the mixed fluid phases and realizing separation of the two-phase signals; or using a selective free radical to selectively enhance the NMR signal of the oil phase or the water phase, thus achieving the polarization enhancement of a single fluid phase in the mixed fluid phases and realizing separation of the oil-water two-phase NMR signals. The method is simple and easy to operate, has a short test time, and can efficiently separate NMR signals of oil and water phases.

An NMR-MAS probehead having an MAS stator (3) receiving an MAS rotor (5) that is surrounded by an RF coil (4) and that has a sample substance, and having a first microwave guide (1) supplying microwave radiation into a sample volume (0) through a coil block (2). The coil block is constructed from dielectric material, is inserted into the wall of the MAS stator so that it surrounds the RF coil and the MAS rotor, and has a first bore (4) that extends coaxially with the longitudinal axis of the elongate MAS rotor, the RF coil being fastened to the inner wall of said first bore, as well as a second bore (8) that extends coaxially with the longitudinal axis of the first microwave guide and has a hollow, elongate second microwave guide (8) supplying microwave radiation from the first microwave guide into the sample volume.

Dynamic nuclear polarization (DNP) agents are provided for DNP nuclear magnetic resonance of analytes. The DNP agents can have the structure A-X-L-R, where A is none or an amphiphilic group; X is a coupling group capable of site-specific binding with the analyte or, when A is an amphiphilic group, capable of site-specific binding with the amphiphilic group; L is a bond or a linker group; and R is a poly-radical group. The poly-radical can be a di-radical, a tri-radical, a tetra-radical, or a combination thereof. Methods of NMR measurement of an analyte comprising an NMR-detectable nucleus are provided. The methods can include the steps of providing a frozen sample containing the analyte and a DNP agent; applying radiation having a frequency that excites electron spin transitions in the DNP agent at an intensity to polarize the NMR-detectable nucleus; and detecting a signal from nuclear spin transitions in the NMR-detectable nucleus.

Dynamic nuclear polarization (DNP) agents are provided for DNP nuclear magnetic resonance of analytes. The DNP agents can have the structure A-X-L-R, where A is none or an amphiphilic group; X is a coupling group capable of site-specific binding with the analyte or, when A is an amphiphilic group, capable of site-specific binding with the amphiphilic group; L is a bond or a linker group; and R is a poly-radical group. The poly-radical can be a di-radical, a tri-radical, a tetra-radical, or a combination thereof. Methods of NMR measurement of an analyte comprising an NMR-detectable nucleus are provided. The methods can include the steps of providing a frozen sample containing the analyte and a DNP agent; applying radiation having a frequency that excites electron spin transitions in the DNP agent at an intensity to polarize the NMR-detectable nucleus; and detecting a signal from nuclear spin transitions in the NMR-detectable nucleus.

Measuring a sample includes providing a magnetic field at the sample using an electromagnetic field resonator. The electromagnetic field resonator includes two or more resonant structures at least partially contained within dielectric material of a substrate, at least a first resonant structure configured to provide the magnetic field at the sample positioned in proximity to the first resonant structure. The sample is characterized by an electron spin resonance frequency. A size of an inner area of the first resonant structure and a number of resonant structures included in the electromagnetic field resonator at least partially determine a range of an operating resonance frequency of the electromagnetic field resonator that includes the electron spin resonance frequency. Measuring the sample also includes receiving an output optical signal from the sample generated based at least in part on a magnetic field generated by the electromagnetic field resonator.

Measuring a sample includes providing a magnetic field at the sample using an electromagnetic field resonator. The electromagnetic field resonator includes two or more resonant structures at least partially contained within dielectric material of a substrate, at least a first resonant structure configured to provide the magnetic field at the sample positioned in proximity to the first resonant structure. The sample is characterized by an electron spin resonance frequency. A size of an inner area of the first resonant structure and a number of resonant structures included in the electromagnetic field resonator at least partially determine a range of an operating resonance frequency of the electromagnetic field resonator that includes the electron spin resonance frequency. Measuring the sample also includes receiving an output optical signal from the sample generated based at least in part on a magnetic field generated by the electromagnetic field resonator.

The present invention relates to compositions and methods using protein reporters as imaging agents in .sup.129Xe NMR and MRI applications. It is described that bla and MBP are genetically-encoded proteins that induce a detectable chemical shift during .sup.129Xe NMR, allowing for use as protein reporters in research and clinical applications.

The present invention relates to compositions and methods using protein reporters as imaging agents in .sup.129Xe NMR and MRI applications. It is described that bla and MBP are genetically-encoded proteins that induce a detectable chemical shift during .sup.129Xe NMR, allowing for use as protein reporters in research and clinical applications.