A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

A SPIM-microscope (Selective Plane Imaging Microscope) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. The SPIM-microscope has an illumination optics having a zoom optics provided in a beam path of the illumination light beam, the zoom optics being adapted to change the focal length of the illumination light beam and adapted to detect a larger area of the object by sequentially detecting sequences of images along the y-direction that have an increased resolution along the z-direction. An image processing unit combines these sequences of images by image stitching into one large overall image.

Disclosed is an optical micro-spectrometry system including an optical microscope, a spectrometry system and an optical system adapted to direct an excitation light beam on the sample through the at least one microscope objective and to collect a Raman or PL light beam from a sample. The optical micro-spectrometry system includes an imaging system configured for acquiring a first image and a second image of the sample, by reflection or transmission of an illumination beam from a sample surface, the first image having a large field of view and the second image having a small field of view, a processing system configured for determining an area in the first image corresponding to the second image, a display system configured for displaying the first image, the second image, and a third image representing the area in overlay on the first image.

A scanner arrangement, suitable for use in laser scanning microscopes, for scanning a scanning field (42) by means of optical radiation (5). It is equipped with at least one scanner (1), which comprises a scanning mirror (2) that is tiltable about one or two scanning axes (3, 4) and means for rotating the scanning field, for scanning a size-variable scanning field and for centring the scanning field centre (43) when panning. The arrangement includes a mechanical pivot device (7) for rotating the scanning field (42). The pivot device is arranged to pivot the scanner (1) about a pivot axis (8). Pivoting is implemented through angular dimensions that correspond to an intended rotation of the scanning field.

The present invention relates to a method for viewing a specimen using a microscope which comprises an objective lens and an image sensor for converting an image formed on the image sensor by the objective lens. A field of view of the microscope can be varied by selecting a section of the image sensor. In one step of the method, an initial image of at least a partial section of the specimen is captured with the microscope, for which a first field of view is selected on the microscope. The initial image is analyzed to determine at least two differing fields of view forming a partial area image, wherein a partial area of the initial image is formed by each of the fields of view forming a partial area image. Images of the partial areas of the specimen are captured for each of the determined fields of view forming a partial area image. The invention further relates to a microscope for viewing a specimen using a microscope.

The present disclosure provides methods for automated slide assessments made in conjunction with digital image-based microscopy. Automated methods of acquiring patient information and specimen information from prepared slides, and digitally linking such information into patient-tagged specimen data, are provided. Also provided are methods that include automatically identifying an optimal area for morphological assessment of a blood smear on a hematological slide, including methods for triggering the analysis of such an area, e.g., using an automated digital image-based hematology system. The present disclosure also provides devices, systems and computer readable media for use in performing processes of the herein described methods.

An optical measurement device is provided. The device includes first and second optical fibers; first and second reaction vessels, and a light guide stage coupled to the first and second optical fibers. The light guide stage is driven to simultaneously optically connect the first and second optical fibers with the first and second reaction vessels. The device includes a measurement device for receiving emissions from the first and second reaction vessels, and a connecting end arranging body that supports the first and second optical fibers along a path. The arranging body is driven along the path between a first position, in which the first optical fiber is optically connected with the measurement device so that light is transmittable from the first reaction vessel, and a second position, in which the second optical fiber is optically connected with the measurement device so that light is transmittable from the second reaction vessel.

A microscope for imaging a sample includes an illumination unit for emitting illumination light to the sample; a detector for capturing a detection light originating from the sample; an optical system for focusing the illumination light onto the sample and focusing the detection light onto the detector; and a scanning unit for scanning the sample using the illumination light. The illumination unit emits the illumination light as separate illumination light beams which can be focused on spatially mutually separated, strip-like sample regions simultaneously. The detector captures the detection light in the form of separate detection light beams originating from the sample regions simultaneously and in a spatially mutually separated manner. The sample regions are in sample planes, and the detector having sub-detectors, which are each assigned to a sample plane and capture a detection light beam that originates from a respective sample plane.

A multi-photon optical probe includes a probe housing, a lateral scanning stage coupled to a lateral mirror assembly and a remote axial scanning stage coupled to an axial mirror assembly. The lateral scanning stage is adapted to scan output laser energy over a planar scan area of the sample by moving the lateral mirror assembly. The axial scanning stage is adapted to scan the output laser energy over a vertical depth range of the sample, which, combined with the planar scan area, forms a 3-dimensional volume. A controller operates in conjunction with a number of remote actuating legs coupled to the axial mirror assembly in order to provide level imaging plane which in turn provides for a clear scanned image.