A microscopic optical imaging system for a living cell, relating to the technical field of living cell culture, observation and detection equipment. The microscopic optical imaging system for a living cell includes a sample stage device, a microscopic optical imaging device, a first linear motion device, a second linear motion device, a third linear motion device, and a worktable device. The microscopic optical imaging device is driven by the first linear motion device to move, the sample stage device is driven by the third linear motion device to move, and the microscopic optical imaging device is driven by the second linear motion device to adjust the resolution for imaging, so that the imaging of living cell samples in regions is realized in a non-contact manner and the resolution for imaging is adjusted; meanwhile, the volume of the microscopic optical imaging system for a living cell is reduced.

An optical imaging system for imaging a target during a medical procedure, the imaging system involving an optical assembly having moveable zoom optics and moveable focus optics, a zoom actuator and a focus actuator for positioning the zoom and focus optics, respectively, a controller for controlling the zoom and focus actuators independently in response to received control input, a camera for capturing an image of the target from the optical assembly, the system capable of performing autofocus during a medical procedure.

An optical measurement unit for a scanning device, a scanning device, and a method for operating a scanning device, for high throughput sample analysis of biological samples are disclosed. An illumination system is used to emit light of at least two different illumination wavelength ranges, and an imaging system is used to detect light of at least two different detection wavelength ranges, in order to detect electromagnetic radiation within a field of view for determining the positioning of a sample within the field of view.

An apparatus for performing photochemical measurements on a liquid or liquid-containing sample includes a light generating system, an optical system configured for directing light from the light generating system towards the sample and a sensing system for measuring at least one optical property of the sample, wherein the light generating system is configured for generating light with intensity levels adjustable within a range, spanning at least three and preferably at least five orders of magnitude; and in that the optical system is configured for directing light from the light generating system towards the sample so as to generate a substantially uniform light intensity level, within a cylindrical region of the sample having a height larger than or equal to five time its radius.

An apparatus for performing photochemical measurements on a liquid or liquid-containing sample includes a light generating system, an optical system configured for directing light from the light generating system towards the sample and a sensing system for measuring at least one optical property of the sample, wherein the light generating system is configured for generating light with intensity levels adjustable within a range, spanning at least three and preferably at least five orders of magnitude; and in that the optical system is configured for directing light from the light generating system towards the sample so as to generate a substantially uniform light intensity level, within a cylindrical region of the sample having a height larger than or equal to five time its radius.

Speckle-modulated line illumination devices and associated methods are described that enable acquisition of images with high resolution and high speed simultaneously. One example speckle-modulated line illumination device includes a spatially coherent light source having a speckled output, a collimation lens positioned to receive the output of the spatially coherent light source, a cylindrical lens positioned to receive a collimated light produced by the collimation lens, and a diffuser positioned to receive a focused line illumination from the cylindrical lens, and to impart random phase variations in light that is output therefrom. The diffuser is coupled to a movement stage to impart rotational or translational movements to the diffusor as a function of time. Implementations of the disclosed technology can be used to develop reflectance confocal microscopy devices and scattering-based light sheet microscopy devices.

Speckle-modulated line illumination devices and associated methods are described that enable acquisition of images with high resolution and high speed simultaneously. One example speckle-modulated line illumination device includes a spatially coherent light source having a speckled output, a collimation lens positioned to receive the output of the spatially coherent light source, a cylindrical lens positioned to receive a collimated light produced by the collimation lens, and a diffuser positioned to receive a focused line illumination from the cylindrical lens, and to impart random phase variations in light that is output therefrom. The diffuser is coupled to a movement stage to impart rotational or translational movements to the diffusor as a function of time. Implementations of the disclosed technology can be used to develop reflectance confocal microscopy devices and scattering-based light sheet microscopy devices.

A 3D optical microscope device of a small form factor optical system is disclosed. A transmission optical system device comprises a first lens having a left side disposed in contact with an input plane, and a second lens having a right side disposed in contact with a rear focal plane and disposed at a position spaced apart by a focal length of the first lens. The first lens and the second lens Fourier-transform a light signal incident on the input plane and output the transformed signal to the rear focal plane.

A laser beam delivery apparatus for a microscope comprises first and second optical diffusers that are configured to move in a periodic manner with a respective different frequency. Each optical diffuser may comprise a spinning disk. The laser light is spatially randomized by the first spinning diffuser and its spatial pattern is further randomized by the second diffuser. The second diffuser prevents any spatial pattern from repeating after one revolution of the first diffuser, which prevents beating patterns from forming when the light is imaged through a spinning confocal disk and increases the uniformity in other cases.

A laser beam delivery apparatus for a microscope comprises first and second optical diffusers that are configured to move in a periodic manner with a respective different frequency. Each optical diffuser may comprise a spinning disk. The laser light is spatially randomized by the first spinning diffuser and its spatial pattern is further randomized by the second diffuser. The second diffuser prevents any spatial pattern from repeating after one revolution of the first diffuser, which prevents beating patterns from forming when the light is imaged through a spinning confocal disk and increases the uniformity in other cases.