The invention relates to a microscope for microscopic examination of a sample comprising a microscope housing enclosing an illumination optics, a microscope stage and an imaging optics, an integrated sample chamber located within the microscope housing and formed by a separated housing section within said microscope housing, the housing section comprising a lid providing direct access to the microscope stage for placing the sample in the sample chamber, wherein said housing section comprises an interface for connection of an external incubation environment conditioning unit to the sample chamber, the interface being configured to provide a connection between said external incubation environment conditioning unit and the sample chamber, such that the environmental conditions in the sample chamber can be controlled when the external incubation environment conditioning unit is connected to the interface.

The invention relates to a heating device for a rack of a diagnose robot comprising: -a polygonal, oval or cylindrical ring (221) defining a polygonal, oval or cylindrical closed wall, having a bottom opening (222) and a top opening (223) -wherein the bottom opening matches the size and form of a filter membrane and/or supporting body (2, 3) of a carrier (1) of a filtration assembly; and -one or more heating elements (224) selected from -a heating element which is integrated into the ring, -a heating element which is arranged on the outer and/or inner surface of the wall defining the ring, and -a heating element arranged in or on a lid, wherein the lid matches the size and form of the opening of the ring.

The invention is directed to a heated stage assembly that can achieve high temperatures (i.e., 40 degrees Celsius and higher and more specifically, 55 degrees Celsius and higher) for use in microscopy including fluorescence microscopy. The microscope stage heater assembly includes a mounting adapter element, a sample holder element mounted within the mounting adapter element, one or more heating elements attached to the sample holder element, and an optical window holder element disposed on a surface of the sample holder element that encloses an optical window element. The optical window element may be in contact with a microscope sample such that the optical window element is heated by the sample holder element to uniformly distribute heat to the specimen.

The invention is directed to a heated stage assembly that can achieve high temperatures (i.e., 40 degrees Celsius and higher and more specifically, 55 degrees Celsius and higher) for use in microscopy including fluorescence microscopy. The microscope stage heater assembly includes a mounting adapter element, a sample holder element mounted within the mounting adapter element, one or more heating elements attached to the sample holder element, and an optical window holder element disposed on a surface of the sample holder element that encloses an optical window element. The optical window element may be in contact with a microscope sample such that the optical window element is heated by the sample holder element to uniformly distribute heat to the specimen.

The invention relates to a method for operating a particle beam apparatus and/or a light microscope, to a computer program product and to a particle beam apparatus and a light microscope, by means of which this method is able to be carried out. The method includes a change in a first temperature of an object, wherein the object is arranged on an object receiving device rendered movable by a motor operated by a supply current. Changing the first temperature of the object alters a second temperature of the object receiving device from a first temperature value to a second temperature value. Further, the method includes changing the supply current of the motor from a first current value to a second current value, wherein the supply current is designed to hold the object receiving device in position, and changing a temperature of the object receiving device from the second temperature value to a third temperature value on account of heat generated by the motor, which is obtained by the second current value of the supply current and fed to the object receiving device. TOT1−15° C.≤TOT3≤TOT1+15° C. applies, where TOT1 is the first temperature value of the object receiving device and where TOT3 is the third temperature value of the object receiving device.

The invention relates to a method for operating a particle beam apparatus and/or a light microscope, to a computer program product and to a particle beam apparatus and a light microscope, by means of which this method is able to be carried out. The method includes a change in a first temperature of an object, wherein the object is arranged on an object receiving device rendered movable by a motor operated by a supply current. Changing the first temperature of the object alters a second temperature of the object receiving device from a first temperature value to a second temperature value. Further, the method includes changing the supply current of the motor from a first current value to a second current value, wherein the supply current is designed to hold the object receiving device in position, and changing a temperature of the object receiving device from the second temperature value to a third temperature value on account of heat generated by the motor, which is obtained by the second current value of the supply current and fed to the object receiving device. TOT1−15° C.≤TOT3≤TOT1+15° C. applies, where TOT1 is the first temperature value of the object receiving device and where TOT3 is the third temperature value of the object receiving device.

The present invention relates to an apparatus for simultaneous imaging and execution of contact-free directed hydrodynamic flow in a specimen with at least one light source, in particular a laser, adapted to dynamically heat the interior and/or a surface of the specimen, a microscope with an objective adapted to image at least a part of the specimen and to guide, in particular focus, a light beam of the light source, in particular a laser beam, into and/or onto the specimen to heat at least one specified location of the specimen, means for manipulating the specified location, and a sample chamber for the specimen that is accessible for imaging radiation and the light beam to allow simultaneous imaging and manipulation of the sample via the objective. Furthermore the present invention is directed to a method for simultaneous imaging and executing contact-free directed hydrodynamic flow in a specimen wherein, at least one light source, in particular a laser, dynamically heats the interior and/or a surface of the specimen via a light beam, in particular via a laser beam, the beam of the at least one light source is directed to the specimen through an objective of a microscope, the light beam is variably guided, in particular focused, to specified locations of the specimen inducing a hydrodynamic flow in the specimen, and imaging the specimen via the same objective as used for introduction of the light beam.

The present invention relates to an apparatus for simultaneous imaging and execution of contact-free directed hydrodynamic flow in a specimen with at least one light source, in particular a laser, adapted to dynamically heat the interior and/or a surface of the specimen, a microscope with an objective adapted to image at least a part of the specimen and to guide, in particular focus, a light beam of the light source, in particular a laser beam, into and/or onto the specimen to heat at least one specified location of the specimen, means for manipulating the specified location, and a sample chamber for the specimen that is accessible for imaging radiation and the light beam to allow simultaneous imaging and manipulation of the sample via the objective. Furthermore the present invention is directed to a method for simultaneous imaging and executing contact-free directed hydrodynamic flow in a specimen wherein, at least one light source, in particular a laser, dynamically heats the interior and/or a surface of the specimen via a light beam, in particular via a laser beam, the beam of the at least one light source is directed to the specimen through an objective of a microscope, the light beam is variably guided, in particular focused, to specified locations of the specimen inducing a hydrodynamic flow in the specimen, and imaging the specimen via the same objective as used for introduction of the light beam.

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.