A method is presented for obtaining an optically-sectioned image of a sample. The method comprises: providing an illumination beam through an imaging lens such that the illumination beam is focused at a focal plane of the imaging lens; obtaining a plurality of images of the sample. Obtaining comprises providing the illumination beam at a plurality of lateral positions on the focal plane and obtaining each image at each lateral position of the illumination beam, such that an intensity of the illumination beam on a portion of the sample at the focal plane varies for each of the plurality of lateral positions. The method further comprises detecting, using a detector, signals collected via the imaging lens; and constructing the optically-sectioned image based on the plurality of images. The constructing comprises: obtaining a plurality of signal values from the portion of the sample from the plurality of images; evaluating a threshold for the portion; and evaluating a pixel value by integrating a fraction of the plurality of signal values based on the threshold.

A system for quantitative differential phase contrast microscopy with isotropic transfer function utilizes a modulation mechanism to create a detection light field having a radial or other axial orientation of optical intensity gradient or other distribution. A condenser generates an off-axis light field to project onto an object under examination, thereby generating an object light field, which is then guided to an image capturing device through an objective lens for capturing images. A differential phase contrast algorithm is applied to the images for obtaining a phase, thereby a depth information corresponding to the phase can be obtained to reconstruct the surface profile of the object.

The present subject matter described an optical microscopy device (2) for a portable imaging system, such as a smartphone. The optical microscopy device (2) comprises an optical lens assembly with ten to sixteen lens elements. The optical lens assembly has an optical magnification in a range of about 1X to about 3X, an airy radius in a range of about 3.2 micron to about 15 micron, a depth of field in a range of about 28 micron to about 133 micron, a numerical aperture in a range of about 0.025 to about 0.176, a half field of view in a range of about 10 degrees to about 39 degrees, and a length in a range of about 6.8 millimeter (mm) to about 18 mm.

Disclosed are a method of acquiring an image using a light-emitting element array and an apparatus therefor. The method of acquiring an image using a light-emitting element array includes reconstructing a first image from some images among source images, detecting a partial region containing a detection target object from the first image, acquiring partial-region images corresponding to the partial region from each of the source images, and reconstructing a second image from the partial-region images using the FPMP.

An optical microscope apparatus includes: a sample interrogation system configured to probe a sample location; and a light collection system configured to collect light output from a sample due to being probed by the sample interrogation system. The light collection system includes: a mirror positioned along an imaging axis that passes through the sample location; and an optical lens system including a plurality of optical lenses arranged along the imaging axis, at least one of the lenses being a multiplet optical lens.

A SPIM-microscope (Selective Plane Imaging Microscopy) and a method of operating the same having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. An electronic zoom is provided that is adapted to change the scanning length in the x-direction independently of a focal length of the illumination light beam and a size of the light sheet in the y-direction and in the z-direction, wherein the number of image pixels in x-direction is maintained unchanged by the electronic zoom independently of the scanning length in x-direction that has been selected.

A system for acquiring microscopic plenoptic images with attenuation of turbulence by a microscope includes, in combination: a sample, the image of which should be obtained, which is able to be treated as a source of chaotic light, whose emission has an intensity profile F(ρs), with ρs planar coordinate on the sample plane; a beam separator; two sensors or detectors, configured to perform the spatial/directional and directional/spatial detection, respectively, in which the planar coordinate on the detector planes is respectively indicated with ρa and ρb; an objective lens, having focal length fO and pupil function PO(ρO), with ρO planar coordinate on the plane of the lens; a second lens, having focal length fT and pupil function PT(ρT), with ρT planar coordinate on the plane of the lens; wherein the second lens is arranged in the optical path (a/b) of the beam transmitted/reflected by the beam separator.

Systems and methods of imaging are described. An imaging system comprises a light source configured to projecting a beam of light; a first diffraction grating configured to separating wavelengths of the projected beam of light; a first lens configured to focusing the wavelengths of the projected beam of light projecting from separated by the first diffraction grating; a reticle configured to altering each wavelength of light focused by the first lens; a second lens configured to collimating the wavelengths of light projecting from altered by the reticle; a second diffraction grating configured to multiplexing the collimated light projecting from collimated by the lens; and a third lens configured to projecting the multiplexed light onto an object plane, wherein the multiplexed light is used to generate an image of an object in the object plane.

The present subject matter described an optical microscopy device (3) for a portable imaging system, such as a smartphone. The optical microscopy device (3) comprises an optical lens assembly with eight to fifteen lens elements. The optical lens assembly has an optical magnification in a range of about 1× to about 7.8×, an airy radius in a range of about 3 micron to about 23.25 micron, a depth of field in a range of about 20 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.115, a half field of view in a range of about 12 degrees to about 30 degrees, and a length in a range of about 6.5 millimeter (mm) to about 57 mm.

A process is provided for imaging a surface of a specimen with an imaging system that employs a BD objective having a darkfield channel and a bright field channel, the BD objective having a circumference. The specimen is obliquely illuminated through the darkfield channel with a first arced illuminating light that obliquely illuminates the specimen through a first arc of the circumference. The first arced illuminating light reflecting off of the surface of the specimen is recorded as a first image of the specimen from the first arced illuminating light reflecting off the surface of the specimen, and a processor generates a 3D topography of the specimen by processing the first image through a topographical imaging technique. Imaging apparatus is also provided as are further process steps for other embodiments.