A method, a device, and a computer program product captures microscopy objects in image data that includes first images recorded with a first contrast and second images recorded with a second contrast, wherein in each case, one of the first and one of the second images can be correspondingly assigned to each other. The method includes capturing information indicating microscopy objects in at least one of the second images, transferring the captured information to those of the first images which correspond to the at least one of the second images, and capturing information indicating microscopy objects in the first images, to which the captured information of the second images was transferred by using the transferred information.

A microscope unit comprises: a main lens barrel of an imaging optical system; and an illumination lens barrel of an illumination optical system connected to the main lens barrel, the illumination optical system having: a collector lens that collects light that has been irradiated from a light source; a fly-eye lens allowing to be transmitted therethrough light from the collector lens; a first relay lens having lenses that relay light from the fly-eye lens; a field stop that stops down a range of light from the first relay lens; a second relay lens that relays to a beam splitter light from the first relay lens; and the beam splitter guiding at least a part of light incident thereon to the objective lens and allowing to be transmitted therethrough to a side of an imaging sensor at least a part of light incident thereon from the objective lens.

A microscope unit comprises: a main lens barrel of an imaging optical system, the main lens barrel being configured capable of being fitted with an imaging sensor and an objective lens; and an illumination lens barrel of an illumination optical system, the illumination lens barrel being connected to the main lens barrel and configured capable of being fitted with a light source, the illumination lens barrel having: a first lens barrel configured capable of being fitted with the light source; and an intermediate lens barrel connecting the main lens barrel and the first lens barrel, and a field stop of light irradiated from the light source being disposed more inwardly than an outer peripheral surface of the intermediate lens barrel is.

A method and microscopy system are useful for recording an image of a sample region. A laser beam is directed onto the sample region with interface(s). An objective lens facilitates images the laser beam on a focusing point which lies on the optical axis of the objective lens or an axis parallel thereto, and which lies in a focusing plane. The objective lens and the sample region are displaced with respect to one another in relative fashion along the optical axis of the objective lens to different relative displacement positions. Intensity values of the laser beam are captured for a respective relative displacement position. A respective highest intensity value for a respective displacement position, a curve of the highest intensity values, and a reference relative displacement position from at least one maximum of the curve, are determined. Image(s) of the sample region is/are captured at the reference relative displacement position.

A device for outcoupling a portion of the radiation of an observation beam path of a binocular eyepiece for documentation or co-observation that is freely selectable at any time. For the outcoupling, a rotatable supporting unit, the axis of rotation of which is parallel to the axes of the observation beam paths, is arranged in the housing having the binocular eyepiece. Three optical elements are arranged on this supporting unit such that an outer and the middle optical element and, after rotation of the supporting unit, the middle and the other outer optical element are each located in one of the observation beam paths. Here, the two outer optical elements have a beam-splitting effect and outcouple a portion of the observation radiation into a common documentation beam path.

Disclosed is a technology for illuminating a specimen in a desired uniform illumination pattern and capturing an image of a wide field of view in a low background illumination environment. Provided, for example, is a fluorescence microscope apparatus including a first illumination optics, a second illumination optics, and an imaging optics. The first illumination optics includes a first light source for exciting fluorescence in a specimen, a spatial light modulation element, and a first illumination optical member for uniformly illuminating the spatial light modulation element. The second illumination optics includes a second illumination optical member for forming an image of a light beam from the spatial light modulation element on a specimen surface. The imaging optics includes an imaging optical member and an imaging element. The imaging optical member captures an image of the specimen surface.

A microscope of the type used by a pathologist to view slides containing biological samples such as tissue or blood is provided with the projection of enhancements to the field of view, such as a heatmap, border, or annotations, substantially in real time as the slide is moved to new locations or changes in magnification or focus occur. The enhancements assist the pathologist in characterizing or classifying the sample, such as being positive for the presence of cancer cells or pathogens.

A surgical microscope for generating an image of an object region includes an eyepiece and an objective conjointly defining a viewing beam path, an image capturing device and a beam path switching device for out-coupling image information. The switching device is switchable between a first switching state wherein light in the viewing beam path is split into a first component along a first beam path to the eyepiece at an intensity IT1 and a second component along a second beam path to the image capturing device at an intensity IT2 and a second switching state wherein the light in the viewing beam path is deflected into the second beam path to the image capturing device at an intensity IU. The switching device includes a beam splitter movable in and out of the viewing beam path and a deflecting element movable into and out of the viewing beam path.

A medical/surgical microscope with two cameras configured to capture two dimensional images of specimens being observed. The medical/surgical microscope is secured to a control apparatus configured to adjust toe-in of the two cameras to insure the convergence of the images. The medical/surgical microscope includes a computer system with a non-transitory memory apparatus for storing computer program code configured for digitally rendering real-world medical/surgical images. The medical/surgical microscope has an illumination system with controls for focusing and regulating the lighting of a specimen. The medical/surgical microscope is configured for real-time video display with the function of recording and broadcasting simultaneously during surgery.

An image acquisition system includes: a first narrowband light source that emits first narrowband light for exciting a luminescent agent that exists in an observation target and emits light having a wavelength belonging to a visible light wavelength band; a second narrowband light source that emits second narrowband light in a wavelength band of ±30 nm of a peak light emission wavelength of the luminescent agent; a broadband light source that emits broadband light for illuminating the observation target; a first image sensor on which an image of light in a light emission wavelength band including a wavelength corresponding to light emitted from the luminescent agent is formed; and a second image sensor including one or more image sensors on which an image of light in a wavelength band other than the light emission wavelength band is formed.