An image processing method includes: generating two images by performing two image enhancement processes for a microscopic image; and generating a corrected image obtained by compositing the two images, wherein the generating the two images includes generating a high-frequency enhanced image in which high-frequency components of the microscopic image are enhanced relative to low-frequency components of the microscopic image that have a lower frequency than the high-frequency components, and generating a microstructure enhanced image in which a microstructure in an observation sample included in the microscopic image is enhanced.

A method for SPIM microscopy with a microscope winch includes (1) an illumination arrangement for illuminating a sample with a substantially planar light sheet, and (2) a detection arrangement for detecting light emitted by the sample with an objective. The sample is displaced through the light sheet in direction of the objective's optical axis, and the sample is illuminated under a first illumination angle and a second illumination angle. A plurality of sample planes are then detected at each illumination angle and stored as at least a first image stack and a second image stack. The image stacks are aligned relative to one another, and are combined in one image stack. A the three-dimensional image stack is projected into a two-dimensional rendering, sample features are aligned, a coordinate transformation is determined, and the coordinate transformation for alignment is applied to the combined image stack.

A digital microscope (1) includes an optical fiber bundle (17) that supplies bright field light, an optical fiber bundle (18) that supplies dark field light, an optical fiber bundle (19) for causing light from a light source to enter the optical fiber bundle (17) (18) and a mechanism for changing a mixture ratio of the bright field light and the dark field light according to operation in an operating section (26). A light entry end of the optical fiber bundle (17) and a light entry end of the optical fiber bundle (18) are arranged adjacent to each other to face in the same direction. A light exit end of the optical fiber (19) is arranged to be opposed to both of the light entry ends.

The invention relates to an apparatus (10) and a method for optically characterizing or processing an object (60), and to an object transport unit (55). The apparatus (10) comprises an object carrier (50) for receiving an object (60); an optical characterization or processing unit (15), comprising at least one device for producing or for receiving light (140) and an objective (40) for exposing the object (60) using the light (140) or for capturing the light (140) from the object (60), wherein the objective (40) has an end face (46) facing the object carrier (50), wherein the end face (46) has an edge (47), wherein the objective (40) further defines an optical axis (502); at least one membrane (100) introduced between the objective (40) and the object carrier (50), wherein the membrane (100) has a portion (120) configured for penetration by the light (140), wherein at least the portion (120) of the membrane (100) is movable in the axial direction with respect to the optical axis (502), at least one membrane holder (80) for holding the at least one membrane (100), and at least one immersion medium (160) which is at least introduced between the membrane (100) and the object carrier (50),
wherein the membrane (100) and the membrane holder (80) are fastened at a point outside of the objective, and wherein the membrane (100) is arranged at the membrane holder (80) in a manner that first contact points (81) between the membrane (100) and the membrane holder (80) are located on or outside a lateral surface (510) which is formed by a geometric extrusion of the edge (47) of the objective (40) parallel to the optical axis (502).

The apparatus (10), the method and the object transport unit (55) facilitate the optical characterization or processing of an object (60) in a manner that meets the specific needs of high-throughput industrial applications.

An augmented reality microscope (ARM) includes an objective lens, an eyepiece, an N-ocular observation tube, where N is a positive integer greater than 2, an image obtaining assembly physically connected to the N-ocular observation tube by a physical interface on the N-ocular observation tube, and an image projection assembly including, an image projection apparatus, a lens apparatus, and a light splitting apparatus. Light generated by an observed object during observation that enters an optical path through the objective lens and light generated by the image projection apparatus that enters the optical path through the lens apparatus converges at the light splitting apparatus in the image projection assembly, the converged light passes through the N-ocular observation tube.

This disclosure discloses a microscope system, a smart medical device, an automatic focusing method, and a storage medium. The smart medical device includes an objective lens, a beam splitter, an image projector assembly, a camera assembly, and a focusing device. The objective lens includes a first end and a second end, and the first end faces a to-be-observed sample. The beam splitter is disposed on the second end. The image projector assembly is in communication with the beam splitter, the image projector assembly includes a first lens and an image projection device, and light generated by the image projector assembly enters the beam splitter through the first lens. The camera assembly includes a camera. The focusing device is disposed on the camera assembly, and the focusing device is configured to perform focus adjustment on the camera.

An imaging system includes a first finite conjugate objective at a frontal end of the system and a second finite conjugate objective at a distal end of the system. The system also includes a beam splitting or merging element positioned between the first finite conjugate objective and the second finite conjugate objective. The system also includes an excitation unit configured to direct an excitation beam into a sample positioned in front of the first finite conjugate objective. The excitation beam is in the form of an excitation plane. The system also includes an image sensor positioned facing the second finite conjugate objective. The image sensor lies in a conjugate plane of an excitation beam illumination plane at the frontal end of the system.

Implementations discussed and claimed herein provide systems and methods live projection imaging for fluorescence microscopy. In one implementation, a 3D view of a sample, such as cells, is generated for direct viewing. A projection of a volume is generated that is optically sheared into a single camera frame in light-sheet fluorescence microscopy. Optical shearing is synchronized with acquisition of a volume, where volumetric information may be directly viewed in a single acquisition to evaluate cellular 3D morphologies and dynamics.

An apparatus is provided for monitoring a focal state of a microscope having an object plane and a main imaging area. The apparatus has an auxiliary light source providing an auxiliary light beam and coupling the auxiliary light beam into the microscope in such a way that the coupled auxiliary light beam runs within a plane which is spanned outside of the main imaging area by a straight line running in the object plane and a normal to the object plane, and that the coupled auxiliary light beam is inclined at an angle to a normal to the object plane. A part of the coupled auxiliary light beam reflected by a reference boundary surface in the microscope impinges on a registration device in an area of incidence. The registration device registers position changes of the area of incidence on the registration device.

The present invention relates to a surgical microscope with a field of view and comprising an optical imaging system which images an inspection area which is at least partially located in the field of view, and to a method for a gesture control of a surgical microscope having an optical imaging system. The surgical microscope further comprises a gesture detection unit for detection of a movement of a surgical instrument, the gesture detection unit having a detection zone which is located between the inspection area and the optical imaging system and is spaced apart from the inspection area, and the gesture detection unit being configured to output a control signal to the optical imaging system depending on the movement of the surgical instrument in the detection zone, the optical imaging system being configured to alter its state depending on the control signal.