A method for configuring a sequence controller of an automated microscope includes, in a learning operating mode, performing training settings in succession. Each training setting corresponds to a respective setting data set of microscope components. A user brings at least a part of the microscope components into positions, and assigns each respective position to one or more examination steps to be performed by the microscope components. The method further includes assessing the training settings after the training settings are performed, and based on the assessment, storing the setting data sets associated with the training settings for subsequent use in the sequence controller, and/or discarding the setting data sets, and/or modifying the setting data sets. The sequence controller specifies a use of the stored setting data sets in the form of a setting of the microscope components in an examination operating mode following the learning operating mode.

A method (400) and a system (200) for generating a chromatically modified image of one or more components on a microscopic slide (303) is disclosed. In one aspect of the invention, the method includes obtaining the image of the one or more components on the microscopic slide (303). Additionally, the method (400) includes processing the image to identify the one or more components. The method (400) further includes segmenting at least one part of the one or more components identified from the image. Furthermore, the method (400) includes chromatically modifying the at least one part of the one or more components and generating a chromatically modified image of the one or more components.

The invention relates to a method in the preparation of samples for microscopic examination onto which a coverslip is applied. The method is notable for the fact that the coverslipping quality is checked automatically and at least partly optically. The invention further relates to an apparatus for carrying out the method, and to an apparatus for checking the coverslipping quality of samples onto which a coverslip is applied.

A system and method for characterization and/or calibration of performance of a multispectral imaging (MSI) system equipping the MSI system for use with a multitude of different fluorescent specimens while being independent on optical characteristics of a specified specimen and providing an integrated system level test for the MSI system. A system and method are adapted to additionally evaluate and express operational parameters performance of the MSI system in terms of standardized units and/or to determine the acceptable detection range of the MSI system.

The invention relates to a method for adjusting and/or calibrating a medical microscope, the following being implemented for at least one observer beam path of the medical microscope: capturing respective image representations of an object at different magnification levels of a zoom optical unit, and determining a zoom center using the captured image representations as a starting point, and i) capturing respective further image representations at different axis positions of at least one linear or rotational movement axis of the medical microscope, a rotation of the capture device relative to the at least one linear or rotational movement axis being determined using the captured further image representations as a starting point, and/or ii) capturing respective further image representations of the object in different focal planes and/or at different working distances in the case of an off-centered imaging optical unit, a rotation of the capture device being determined using the captured further image representations as a starting point, and a reference marking being determined using the determined zoom center and the determined rotation as a starting point and being provided for adjustment and/or calibration purposes. Further, the invention relates to a medical microscope.

A super resolution technique, intended mainly for fluorescence microscopy, acquires the three-dimensional position of an emitter, through a hybrid method, including a number of steps. In a first step the two-dimensional position of an emitter is acquired, using a technique, named in this application as an Abbe's loophole technique. In this technique a doughnut, or a combination of distributions, having a zero intensity at the combined center of the distributions, is projected onto the sample containing the emitter, under conditions wherein the doughnut null is moved towards the emitter to reach a position in which the emitter does not emit light. In a second step, an axial measurement is obtained using a 3D shaping method, characterized by the fact that the emitted light is shaped by an additional optical module creating a shape of the light emitted by the emitter, this shape being dependent of the axial position and means to retrieve the axial position from the shape.

A control apparatus includes: an acquisition unit configured to acquire an operation instruction made by a voice input to an imaging device including: an optical system including a focus lens; and an image sensor; and a controller configured to control the focus lens moving at a first velocity to stop movement when the operation instruction is an instruction to stop an operation of the focus lens, and control the focus lens to move at a second velocity lower than the first velocity.

Apparatus and methods are described for use with a blood sample. Using a microscope (24), three images of a microscopic imaging field of the blood sample are acquired, each of the images being acquired using respective, different imaging conditions, and the first one of the three images being acquired under violet-light brightfield imaging. Using at least one computer processor (28), an artificial color microscopic image of the microscopic imaging field is generated, by mapping the first one of the three images to a red channel of the artificial color microscopic image, mapping a second one of the three images to a second color channel of the artificial color microscopic image, and mapping a third one of the three images to a third color channel of the artificial color microscopic image. Other applications are also described.

The present invention relates to a method of reading out information from a data carrier and to a data carrier utilizing the concept of structured-illumination microscopy or saturated structured-illumination microscopy.

A microscopy imaging system comprises a fluorescence lifetime imaging microscopy (FLIM) system comprising a pulsed light source configured to direct a plurality of excitation light pulses onto a sample, a photo detector configured to detect emitted fluorescent photons created by the plurality of excitation pulses interacting with the sample, and a FLIM data acquisition system configured to measure the time interval between the excitation light pulses and the detected emitted fluorescent photons, a scanning light microscopy (SLM) system comprising a SLM data acquisition system, a fast scanning mirror and a slow scanning mirror, wherein the mirrors are configured to scan the light pulses across the sample; and a data processing system communicatively connected to the FLIM and SLM systems. Microscopy imaging methods are also disclosed.