Provided are a fiber bundle image processing method (200) and an apparatus. The method (200) includes: determining pixel information corresponding to a center position of a fiber in a sample image; correcting the determined pixel information; and reconstructing the sample image based on the corrected pixel information to obtain a reconstructed image. The method (200) and apparatus can not only obtain a more ideal fiber-bundle processed image, but also have a smaller calculation amount, and the entire calculation process takes less time.

An imaging system includes an imaging device configured to image an imaging subject on an imaging optical axis, and a calculation unit configured to acquire data relating to a position and/or a size of the imaging subject based on image information acquired by the imaging device through the imaging. The calculation unit acquires change information relating to condition change in the imaging and/or change in the imaging subject on the image. The change is caused by interposition of a light transmitting member when the light transmitting member is interposed on the imaging optical axis during the imaging, and the calculation unit corrects the data based on the change information.

The image processing method according to the present application includes: acquiring a medical image captured by an imaging apparatus; and determining an intensity of a filter to be applied to the medical image according to a degree of focusing of the medical image.

An automatic focus system for an optical microscope that facilitates faster focusing by using at least two offset focusing cameras. Each offset focusing camera can be positioned on a different side of an image forming conjugate plane so that their sharpness curves intersect at the image forming conjugate plane. Focus of a specimen can be adjusted by using sharpness values determined from images taken by the offset focusing cameras.

Projection of visible, non-treatment light onto an eye to illuminate specific areas of the surgical field is disclosed herein. A surgical system may include a surgical console; a microscope communicatively coupled to the surgical console; a camera communicatively coupled to the surgical console; and a projector operable to project light onto an eye. The projector may be communicatively coupled to the surgical console. A method for light projection may include collecting information from an eye using a camera; determining the light projection based, at least in part, on the collected information; and projecting visible, non-treatment light onto the eye using a projector.

A computer implemented system and method for generating a focus corrected image of a sample disposed on a sample holder of an imaging system is disclosed. The imaging system includes an image sensor and a lens moveable relative to the image sensor between a first position and a second position. A characteristic map of the lens is developed that associates coordinates of each pixel of an image generated by the imaging sensor with one of a first plurality of locations of the lens between the first position and the second position. An image generator develops an output pixel of a focus-corrected image of a sample from a plurality of images of the sample acquired when the lens is positioned at a corresponding one of a second plurality of locations of the lens between the first position and the second position. The image generator selects a second location in accordance with the characteristic map, an image from the plurality of images of the sample associated with the second location, and determines a value of the output pixel in accordance with a value of a pixel of the selected one of the plurality of images that corresponds to the output pixel.

Disclosed is a technology for illuminating a specimen in a desired uniform illumination pattern and capturing an image of a wide field of view in a low background illumination environment. Provided, for example, is a fluorescence microscope apparatus including a first illumination optics, a second illumination optics, and an imaging optics. The first illumination optics includes a first light source for exciting fluorescence in a specimen, a spatial light modulation element, and a first illumination optical member for uniformly illuminating the spatial light modulation element. The second illumination optics includes a second illumination optical member for forming an image of a light beam from the spatial light modulation element on a specimen surface. The imaging optics includes an imaging optical member and an imaging element. The imaging optical member captures an image of the specimen surface.

A stereo microscope includes a stand, two optical image acquisition units configured to connect to the stand to capture a stereoscopic image, which define an imaging plane using two optical axes of the image acquisition units, a pair of video glasses including two optical image reproduction units, each having an optical axis and a display for reproducing an image, which together define an image plane, wherein the optical image reproduction units are arranged to produce a stereoscopic image impression, and two optical axes of the optical image reproduction units define an image reproduction plane, a detection device configured to determine spatial orientation of the video glasses, the image reproduction plane, the image plane and the imaging plane, and a control unit configured to pivot the stand so that the intersection lines of the image plane and the imaging plane on the image reproduction plane are made parallel. Methods are also disclosed.

Subpixel line scanning. A slide scanning device comprises a plurality of line sensors (112a, 112b, 112c), each comprising a plurality of pixel sensors. Each line sensor is offset from an adjacent line sensor by a fraction of a length of each pixel sensor, and generates a line image of the same field of view at its respective offset. For each of a plurality of positions on a sample, a processor combines the line images of the same field of view, generated by the plurality of line sensors at their respective offsets, to produce a plurality of subpixels for each of at least a subset of pixels within the line images of the same field of view, and generates an up-sampled line image of the position comprising the plurality of subpixels. Then, the processor combines the up-sampled line images of each of the plurality of positions on the sample into an image.

A control system for automatedly determining an illumination intensity of at least one light source of a fluorescence microscope is provided. The control system is configured to automatedly determine, after a change in a light path, a control value for the illumination intensity of the at least one light source in order to achieve a desired value of an inspection parameter characterizing sample inspection. The light path comprises at least one of: an illumination path from the at least one light source to the sample and an imaging path from the sample to at least one detector. Determining the control value is based on: (i) a value of the illumination intensity that was set before the change in the light path, (ii) a value of the inspection parameter used before the change in the light path, and (iii) a physical model of the light path.