A microscope system includes a microscope that acquires a microscopic image and circuitry. The circuitry causes the microscope to perform a series of image-shooting operations for acquiring a plurality of microscopic images and outputs an access code generated in response to the start of the series of image-shooting operations, the access code being used to access image-shooting information pertaining to the series of image-shooting operations.

A three-dimensional displacement compensation method is provided. The method includes an obtaining step, a transforming step, a first determining step, a first calculating step and a compensating step. The obtaining step includes obtaining a current image of a measured element captured by a microscopic thermoreflectance thermography device. The transforming step includes two sub-steps. One sub-step uses Fourier transform to calculate a reference image to obtain a first result, and the other sub-step uses Fourier transform to calculate the current image to obtain a second result. The first determining step includes determining a peak point coordinate and a fitting diameter of a point spread function of an optical system of the device. The first calculating step includes calculating a three-dimensional displacement of the position to be compensated relative to the reference position. The compensating step compensates the position to be compensated.

The patent discloses a differential phase contrast (DPC) quantitative phase microscopy method based on the optimal illumination pattern design. Firstly, the optimal illumination pattern corresponding to the isotropic phase transfer function of DPC quantitative phase imaging is derived, which is determined as a semi-annular illumination pattern with the illumination numerical aperture NA.sub.ill equal to the numerical aperture NA.sub.obj of the objective lens. The illumination intensity distribution varies with the cosine of the illumination angle, and it can be expressed as S(θ)=cos(θ). This patent effectively compensates for the frequency loss of phase transfer, not only the high-frequency responses of PTF are enhanced, but also the transfer responses of low-frequency phase information is significantly improved. As a result, the optimal illumination scheme ensures the correctness and achieves high resolution phase reconstruction, while the number of illuminations is reduced to a minimum of two, which greatly increases the imaging speed, allowing for real-time dynamic, high-correctness, high-resolution phase imaging results.

A computer-implemented method of reviewing digital pathology data may include receiving a digital pathology image into a digital storage device, the digital pathology image being associated with a patient, providing for display the digital pathology image on a display, pairing the digital pathology image with a physical token of the digital pathology image in an interactive system, receiving one or more commands from the interactive system, determining one or more manipulations or modifications to the displayed digital pathology image based on the one or more commands, and providing for display a modified digital pathology image on the display according to the determined one or more manipulations or modifications.

The invention relates to a ptychographic imaging system, comprising a plurality of light sources adapted to emit light onto a sample location, wherein said light sources are arranged in a predefined pattern; and a controller adapted to control operation of said plurality of light sources; wherein at least one of a) said predefined pattern of the light sources and b) said control operation of the plurality of light sources is adapted to compensate for geometric effects due to an arrangement of the light sources relative to the sample location.

An optical microscope device includes: a first illumination optical system including a light source that emits illumination light for illuminating a specimen an LCOS spatial light modulation element that controls a polarization state of the illumination light, a first illumination optical member that uniformly illuminates the LCOS spatial light modulation element, and a polarization optical element that controls a transmission state of the illumination light directed to the specimen from the LCOS spatial light modulation element in response to the polarization state of the illumination light; a second illumination optical system including a second illumination optical member that images a light flux from the first illumination optical system on a specimen surface; and an imaging optical system for imaging the specimen surface.

Systems and methods are provided for multi-spectral or hyper-spectral fluorescence imaging. In one example, a spectral encoding device may be positioned in a detection light path between a detection objective and an imaging sensor of a microscope. In one example, the spectral encoding device includes a first dichroic mirror having a sine transmittance profile and a second dichroic mirror having a cosine transmittance profile. In addition to collecting transmitted light, reflected light from each dichroic mirror is collected and used for total intensity normalization and image analysis.

An information processing apparatus include a processor configured to: control a display to display at least a partial image of a captured image generated by capturing an image of an observation target; generate field of view information by associating: display position information indicating a position of a display area corresponding to the displayed partial image; magnification information indicating a display magnification of the partial image; and time information indicating a display time of the partial image; record the field of view information in a memory; and extract a piece of the field of view information including the magnification information indicating at least a single kind of specific display magnification input to an input device from among pieces of the field of view information recorded in the memory; generate a field of view map image corresponding to the extracted piece of field of view information; and control the display to display the field of view map image.

A control device for controlling a microscope includes: a computation module. The control device: adjusts a sample volume velocity of a sample volume of the microscope relative to the microscope; and adjusts a light sheet velocity of the light sheet of the microscope relative to the microscope based on the sample volume velocity.

A microscope system includes an objective lens, configured to gather a first light of a target sample to enter a first optical path, wherein the first light converges, at a beamsplitter, with a second light generated by an image projection module after entering the first optical path through a lens assembly; a beamsplitter assembly, configured to respectively separate and cast light in different optical paths; a camera assembly, configured to photograph the target sample in a microscope field of view, to photograph a clearly focused image through a first optical path by using the camera assembly; an auxiliary focusing device, configured to determine a focal length matching the camera assembly; and a focusing device, configured to adjust a focal length of image light entering the camera assembly according to a defocus amount of a target sample image determined by the auxiliary focusing device.