A external accessory can allow a mobile device to perform microscopy imaging with Type-C ultraviolet (UVC) light excitation. The external accessory includes a compound lens placed in front of a camera lens of the mobile device. A light transparent optical window configured to be placed in front of the compound lens and positioned such that a front surface of the optical window overlaps the front focal plane of the compound lens. One or more light emitting diode (LED) that emits UVC light positioned at one or more side-edges of the optical window. The one or more LED emits UVC light through the optical window so that the UVC light undergoes total internal reflection. An externally triggered LED driver configured to power and control the LED(s).

Described herein are methods for imaging a fluorescent bioassay (including a substrate, such as dispersed cellular sample, exposed to one or more exogenous fluorophore and/or fluorescent probe that accumulate in a structure of interest). The bioassay can be excited with Type-C ultraviolet (UVC) light produced by one or more light emitting diode (LED). The UVC can have a center wavelength that causes emission by the fluorescent bioassay. A digital optical device can collect a signal emitted from the fluorescent bioassay in response to the excitation. The methods relate in particular to Microscopy with Ultraviolet Surface Excitation (MUSE) imaging.

An automated rapid on-site evaluation (ROSE) system for smearing, staining and capturing a digital image of a biological sample such as a fine needle aspiration (FNA) sample is presented. The system comprises a housing with a user interface monitor or screen, a smearing system, a staining system, and an image capture system. The housing can further include a receiver for a biological sample that can direct the sample to a sample slide. The smearing system is configured to smear the sample on the sample slide. The staining system is configured to stain the sample. The image capture system is configured to capture a magnified image of the sample and store the digital image in the housing or remotely. The digital images may be available immediately for local or off-site review. The wait time between staining the sample and image capture can be less than 60 seconds.

A magnified observation apparatus includes an FOV changer, an in-focus degree evaluator, and a focus sequence executor. The in-focus degree evaluator calculates in-focus degree feature quantities, which represent in-focus degrees of image data corresponding to images to be displayed by a display controller on a display. The focus sequence executor executes an FOV-moving focus sequence in which a focusing device adjusts a relative distance between a focus position of the objective lens and an observation object in accordance with in-focus degree feature quantities of image data that are successively calculated by the in-focus degree evaluator corresponding to images of the observation object that are captured during movement of an observation FOV by the FOV changer so that a live image of the observation object is displayed on the display based on image data that is obtained during the movement of an observation FOV by the display controller.

A confocal microscope is provided that includes one or more lasers focused by an optical system into a line on the surface of a sample mounted to a stage. The microscope further includes, at least one linear array detector that is optically conjugated to the focused line. The stage permits movement of the sample with respect to all other components of the microscope, which remain stationary.

The present invention relates to a surgical microscope with a field of view and comprising an optical imaging system which images an inspection area which is at least partially located in the field of view, and to a method for a gesture control of a surgical microscope having an optical imaging system. The surgical microscope further comprises a gesture detection unit for detection of a movement of a surgical instrument, the gesture detection unit having a detection zone which is located between the inspection area and the optical imaging system and is spaced apart from the inspection area, and the gesture detection unit being configured to output a control signal to the optical imaging system depending on the movement of the surgical instrument in the detection zone, the optical imaging system being configured to alter its state depending on the control signal.

An optical imaging system for imaging a target during a medical procedure, the imaging system involving an optical assembly having moveable zoom optics and moveable focus optics, a zoom actuator and a focus actuator for positioning the zoom and focus optics, respectively, a controller for controlling the zoom and focus actuators independently in response to received control input, a camera for capturing an image of the target from the optical assembly, the system capable of performing autofocus during a medical procedure.

A microscope apparatus includes: a sample setting unit in which a sample is set; an imaging unit configured to image the sample set in the sample setting unit; a housing unit on which the sample setting unit is arranged, and which is configured to internally accommodate the imaging unit; a first light source configured to irradiate light for fluorescence excitation on the sample in the sample setting unit; a first cover configured to be movable to a first position that covers the sample setting unit and a second position that opens the sample setting unit; and a second cover configured to be movable within the first cover; and a second light source arranged in a space covered with the second cover and configured to irradiate light on the sample in the sample setting unit.

A method for approximating image data is disclosed. The method includes obtaining initial image data formed by imaging at least part of a sample and analysing the initial image data to form one or more portions of image data. The method also includes accessing a set of predefined stereotype elements , determining one or more of the stereotype elements as a visual approximation for the at least one object within the sample, selecting one or more of the determined stereotype elements to be associated with at least one object within the sample, and forming instruction data based on and/or including the one or more selected stereotype elements, the instruction data including instructions on how to arrange the one or more selected stereotype elements, to a processing system for approximating image data and to a system comprising such processing system.

A three-dimensional viewing includes a pair of digital image sensors in communication with a pair of digital displays and controlled by a pair of processors to render three-dimensional images of samples on the displays.