A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

The present invention discloses a method for detecting a microstructure of a functionally graded material based on digital acousto-optic holography, including the following steps: excite a sample with an ultrasonic wave; record a light wave; form a single tomographic acousto-optic hologram; perform numerical reconstruction of phase information, and perform global detection. The present invention uses an acoustic-optic modulation device to modulate a laser light source of a laser of a laser device to form two light waves of different frequencies. The two light waves each constitute a Mach-Zehnder interference system to record reflection wave information and transmission wave information of an ultrasound, and are finally combined and recorded in the same hologram to form the single tomographic acousto-optic hologram.

A system for analyzing a transparent particle including: an analysis pathway, including a first light source emitting an analysis light beam, and a first optical system focusing the analysis light beam in a focusing plane; and a position control pathway including a second light source, an image sensor, and a second optical system at least partially merged with the first optical system. The image sensor is offset relative to the image of the focusing plane by the second optical system. The system makes it possible to control correct positioning of the particle, even though it is transparent, and without disturbing the analysis pathway.

The current invention concerns a sample vial for receiving a liquid cell sample, to be used in conjunction with a digital holographic microscope (DHM), said sample vial comprises at least two compartments in fluid connection with one another, said compartments comprising at least one pair of screening surfaces, said screening surfaces are essentially flat; and characterized in that the distance between the pair of screening surfaces of the second compartment is smaller than the distance between the pair of screening surfaces of the first compartment. In a second and third aspect, the current invention pertains to a method and system for analyzing a liquid cell sample by DHM, employing the sample vial of the current invention.

An optical observation system includes a spatial light modulator displaying a Fresnel type kinoform on a phase modulation plane, and modulating light L1 in phase to irradiate an observation object with modulated light L2, an imaging optical system imaging observation target light L3 from the observation object, an optical system moving mechanism moving the imaging optical system in an optical axis direction of the observation target light L3, and a control section controlling the optical system moving mechanism such that the focal position of the imaging optical system changes in response to a change in the light condensing position of the modulated light L2 by the Fresnel type kinoform.

An ultra-high-speed 3D refractive index tomography and structured illumination microscopy system using a wavefront shaper and a method using the same are provided. A method of using an ultra-high-speed 3D refractive index tomography and structured illumination microscopy system that utilizes a wavefront shaper includes adjusting an irradiation angle of a plane wave incident on a sample by using the wavefront shaper, measuring a 2D optical field, which passes through the sample, based on the irradiation angle of the plane wave, and obtaining a 3D refractive index image from information of the measured 2D optical field by using an optical diffraction tomography or a filtered back projection algorithm.

Provided are a morphological cell parameter-based erythrocyte test method and digital holographic microscope used therein, and the morphological cell parameter-based erythrocyte test method includes performing modeling to create a 3D image of an erythrocyte to be tested and measuring morphological parameters of the erythrocyte based on the 3D image.

The morphological cell parameter-based erythrocyte test method performs modeling of a 3D image for an erythrocyte to be tested and measures morphological parameters of the erythrocyte based on the 3D image. Therefore, time and effort consumed in measurement may be reduced, and accuracy of the measurement is excellent.

A generation method of a digital hologram includes steps of emitting coherent light from a coherent light source, imaging a hologram that is an interference pattern of an object beam and a reference beam due to the emission light from the light source, and setting a plurality of wavelengths of the illumination light that generates the hologram detected by the detector, and wherein the plurality of wavelength are specified by the wavelength setting step based on a magnification percentage X of a conjugate image set up by a user not to disturb visibility of an image when a real image and the conjugate image reconstructed by a predetermined calculation means relative to structures of observation targets are superimposed to a corresponding real image so that a shortest wavelength λ.sub.min and a longest wavelength λ.sub.max satisfy the expression λ.sub.max/λ.sub.min≧(1/X+1).

Systems and methods for uniquely identifying fluid-phase products by endowing them with fingerprints composed of dispersed colloidal particles, and by reading out those fingerprints on demand using Total Holographic Characterization. A library of chemically inert colloidal particles is developed that can be dispersed into soft materials, the stoichiometry of the mixture encoding user-specified information, including information about the host material. Encoded information then can be recovered by high-speed analysis of holographic microscopy images of the dispersed particles. Specifically, holograms of individual colloidal spheres are analyzed with predictions of the theory of light scattering to measure each sphere's radius and refractive index, thereby building up the distribution of particle properties one particle at a time. A complete analysis of a colloidal fingerprint requires several thousand single-particle holograms and can be completed in ten minutes.

Microscope (2) comprising a coherent light source (4) producing a coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14) configured to split the coherent light beam (7) into a reference beam (7a) and a sample illumination beam (7b), a sample holder (18) configured to hold a sample (1) to be observed, a sample illumination device (28) configured to direct the sample illumination beam (7b) through the sample and into a microscope objective (37), a beam reuniter (16) configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system (8) configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.