An optical fiber splitter device comprising at least two optical fibers of different lengths is disclosed for partial or complete compensation of the optical path difference between waves interfering to generate a hologram or an interferogram. Various implementations of this fiber splitter device are described in apparatuses for holographic and interferometric imaging of microscopic and larger samples.

An optical apparatus includes a plurality of lenses including a first lens affixed to an optics mount holder which is adjustably affixed to an alignment channel part. The optical axis of the first lens passes through a hollow area of the alignment channel part. The lens is mounted on the optics mount holder which is adjustably affixed to one end of the alignment channel part with a plurality of adjustable fasteners. A method is provided for aligning the optical apparatus.

The invention concerns a device for the holographic and hyperspectral measurement and analysis (2) of a sample (3), comprising; —an acquisition means (2) for acquiring a diffracted image (11) of an image of the sample (3); and interference patterns (12) of a reference light signal (R) and the light signal (O) having passed through the sample (3) to be measured and analysed; and—a means for illuminating the sample (3) focused on the sample (3); and—a means for reconstructing and analysing (1) the hyperspectral holographic image comprising a deep convolutional neural network generating an image for analysis and detection of particularities in the sample.

A method comprising determining a quantitative dispersion image of an object based on a set of quantitative phase images, each quantitative phase image of the set of quantitative phase images having been obtained with a respective different illumination light wavelength.

In various embodiments a module for generating an interference pattern for producing a digital holographic image is provided. The module comprises an adaptive lens arrangement configured to receive, from a microscope, an object wave of an intermediate image of a sample to be examined, and to generate an adapted object wave of the intermediate image of the sample by reducing a curvature of the object wave of the intermediate image; a reference input interface configured to receive an optical fiber delivering a reference wave from the coherent light source to the module and an interference arrangement configured to generate an interference pattern to be received by an imaging sensor arrangement, wherein the interference pattern is based on the adapted object wave and the reference wave from a coherent light source; wherein a position of the reference input interface of the module is configured to be adjustable with respect to at least two directions (x-y), wherein at least one of the adjustable directions is in parallel to a propagation direction of the reference wave leaving the optical fiber.

The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

A device for detecting at least one object present in a sample, the device including a light source to emit at least one incident wave at a wavelength λ, a detection volume intended to receive the object, and to receive at least one incident wave, an image sensor positioned to receive at least one scattered light wave obtained by diffraction of the incident wave on the object and a reference wave from the source and not diffracted on the object and to generate a holographic image, and a computer data processing device to digitally reconstruct the object based at least on the holographic image and the wavelength λ. The device also comprises a support comprising patterns organized to form at least one diffraction grating, the grating being periodic and having a pitch P, such that λ/2≤P≤2λ.

A machine learning-based digital holography device and a method for operating same are disclosed. The digital holography method according to one embodiment comprises the steps of: obtaining an optical image including two-dimensional information about a sample; and on the basis of the optical image, generating a holographic image including three-dimensional information about the sample.

Provided are an improved holographic reconstruction apparatus and method. A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; extracting reference light information from the obtained object hologram; calculating a wavenumber vector constant of the extracted reference light information, and generating digital reference light by calculating a compensation term of the reference light information by using the calculated wavenumber vector constant; extracting curvature aberration information from the object hologram, and then generating digital curvature in which a curvature aberration is compensated for; calculating a compensated object hologram by multiplying the compensation term of the reference light information by the obtained object hologram; extracting phase information of the compensated object hologram; and reconstructing 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object by calculating the quantitative thickness information of the measurement target object by using the extracted phase information of the compensated object hologram.

A low-cost digital holography microscope (DHM) that is capable of performing inspection at high speed while accurately inspecting an inspection object at high resolution, an inspection method using the DHM, and a method of manufacturing a semiconductor device by using the DHM are provided. The DHM includes: a light source configured to generate and output light; a beam splitter configured to cause the light to be incident on an inspection object and output reflected light from the inspection object; and a detector configured to detect the reflected light, wherein, when the reflected light includes interference light, the detector generates a hologram of the interference light, and wherein no lens is present in a path from the light source to the detector.