Provided are an improved holographic reconstruction apparatus and method. A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; extracting reference light information from the obtained object hologram; calculating a wavenumber vector constant of the extracted reference light information, and generating digital reference light by calculating a compensation term of the reference light information by using the calculated wavenumber vector constant; extracting curvature aberration information from the object hologram, and then generating digital curvature in which a curvature aberration is compensated for; calculating a compensated object hologram by multiplying the compensation term of the reference light information by the obtained object hologram; extracting phase information of the compensated object hologram; and reconstructing 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object by calculating the quantitative thickness information of the measurement target object by using the extracted phase information of the compensated object hologram.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

A lensless holographic imaging system having a holographic optical element includes: a coherent light source for outputting a first light beam and a second light beam, wherein the first light beam irradiates a first inspection plane to form first object-diffracted light; a light modulator for modulating the second light beam into reading light having a specific wavefront; a multiplexed holographic optical element, wherein the first object-diffracted light passes through the multiplexed holographic optical element, and the reading light is input into the multiplexed holographic optical element to generate a diffracted light beam as system reference light; and an image capture device for reading at least one interference signal generated by interference between the first object-diffracted light and the system reference light. The lensless holographic imaging system has a relatively small volume and relatively high diffraction efficiency.

An optical filter to increase contrast of an image generated with a spatial light modulator includes a lens for spatially Fourier transforming modulated light from the spatial light modulator, and an optical filter mask positioned at a Fourier plane of the lens to filter the modulated light. The modulated light has a plurality of diffraction orders, and the optical filter mask transmits at least one of the diffraction orders of the modulated light and block a remaining portion of the modulated light. A method that improves contrast of an image generated with a spatial light modulator includes spatially Fourier transforming modulated light from the spatial light modulator onto a Fourier plane, and filtering the modulated light by transmitting at least one diffraction order of the modulated light at the Fourier plane and blocking a remaining portion of the modulated light at the Fourier plane.

Embodiments of the present disclosure relate to a method for analyzing an imaging quality of an imaging system. The imaging system comprises a spatial light modulator. The spatial light modulator comprises pixel units arranged in an array. The method comprises obtaining a transmittance distribution function of the spatial light modulator based on a structural parameter of the pixel unit, wherein the structural parameter is an aperture ratio. The imaging quality analysis parameter of the imaging system is obtained based on the transmittance distribution function of the spatial light modulator. Then, the imaging quality of the imaging system is analyzed based on the imaging quality analysis parameter.

A method for producing a hologram (1), (1) for security elements (1a) and/or security documents (1b). One or more virtual hologram planes (10) are arranged in front of and/or behind one or more virtual models (20) and/or one or more virtual hologram planes (10) are arranged such that they intersect one or more virtual models (20). One or more virtual light sources (30) are arranged on one or more partial regions of the surface (21) of one or more of the virtual models (20). One or more virtual electromagnetic fields (40) are calculated starting from at least one of the virtual light sources (30) in one or more zones (11) of the one or more virtual hologram planes (10). In the one or more zones (11), in each case, a virtual total electromagnetic field (41) is calculated on the basis of the sum of two or more, of the virtual electromagnetic fields (40) in the respective zone (11). One or more phase images (50) are calculated from the virtual total electromagnetic fields (41) in the one or more zones (11). A height profile (60) of the hologram (1) is calculated from the one or more phase images (50) and the height profile (60) of the hologram (1) is incorporated into a substrate (2) to provide the hologram (1).

Provided are an improved holographic reconstruction apparatus and method.

A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; extracting reference light information from the obtained object hologram; calculating a wavenumber vector constant of the extracted reference light information, and generating digital reference light by calculating a compensation term of the reference light information by using the calculated wavenumber vector constant; extracting curvature aberration information from the object hologram, and then generating digital curvature in which a curvature aberration is compensated for; calculating a compensated object hologram by multiplying the compensation term of the reference light information by the obtained object hologram; extracting phase information of the compensated object hologram; and reconstructing 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object by calculating the quantitative thickness information of the measurement target object by using the extracted phase information of the compensated object hologram.