A device and method for sorting objects immersed in a flowing medium are disclosed. An example device comprises a holographic imaging unit comprising one or more holographic imaging elements, a fluid handling unit comprising one or more microfluidic channels configured to conduct flowing medium along a corresponding holographic imaging element and at least one microfluidic switch arranged downstream of an imaging region in the microfluidic channel configured to direct objects in the flowing medium into a one of a plurality of outlets. The example device also comprises a processor configured to determine real-time characterizations of holographic diffraction images obtained for the moving objects. The processing unit is further configured to control the at least one microfluidic switch in response to the real-time characterizations.

The invention concerns a device for the holographic and hyperspectral measurement and analysis (2) of a sample (3), comprising; an acquisition means (2) for acquiring a diffracted image (11) of an image of the sample (3); and interference patterns (12) of a reference light signal (R) and the light signal (O) having passed through the sample (3) to be measured and analysed; and a means for illuminating the sample (3) focused on the sample (3); and a means for reconstructing and analysing (1) the hyperspectral holographic image comprising a deep convolutional neural network generating an image for analysis and detection of particularities in the sample.

Example apparatuses and methods relating to imaging systems are provided. An example imaging system may include an optical source configured to generate an optical beam, a beam splitter configured to split the optical beam into a reference beam and an object beam, and a beam combiner configured to route a combined beam with reference beam and object beam components along a common path into a target medium. In this regard, the target medium may act upon the combined beam to form a common path interference beam. The example imaging system may further include an imaging sensor configured to receive the common path interference beam and generate common path interference beam data associated with the common path interference beam, and an image data processor configured to analyze the common path interference beam data to generate image data describing the target medium.

A neural imaging system may include an imaging array, an image data processor operably coupled to the imaging array to process image data received from the imaging array, and a beam angle separator disposed between the imaging array and an object being imaged. The beam angle separator may be configured to separate an object beam reflected from the object being imaged into a plurality of reference beams each having different angular separation with respect to the object beam. The image data processor may be configured to generate image data of the object for each one of the reference beams to correspond to a respective different depth within the object.

A device and method for sorting objects immersed in a flowing medium are disclosed. An example device comprises a holographic imaging unit comprising one or more holographic imaging elements, a fluid handling unit comprising one or more microfluidic channels configured to conduct flowing medium along a corresponding holographic imaging element and at least one microfluidic switch arranged downstream of an imaging region in the microfluidic channel configured to direct objects in the flowing medium into a one of a plurality of outlets. The example device also comprises a processor configured to determine real-time characterizations of holographic diffraction images obtained for the moving objects. The processing unit is further configured to control the at least one microfluidic switch in response to the real-time characterizations.

An apparatus and method are introduced to produce a hologram of an object from electromagnetic radiation, such as incoherent light, received from the object. The electromagnetic radiation is received by a receiving assembly and transformed into a plurality of co-linear co-propagating beams with different focal distances. The interference of the plurality of beams is enabled by projecting components of each beam along a common polarization direction. The interference patterns thus formed are recorded and then processed to form the hologram of the object.

A method for light detection and ranging that includes forming a first light pattern within a region of a scene by holographic projection. The first light pattern includes n light spots arranged in a regular array. A light return signal is received from each light detection element of an array of light detection elements directed at the region of the scene. The intensity of the light return signals is assessed. If the light return signals do not meet at least one signal validation criterion, a second light pattern is formed within the region of the scene by holographic projection. The second light pattern includes m light spots arranged in a regular array, wherein mn. A time-of-flight in association with each light spot of the second light pattern is then determined.

An apparatus and method are introduced to produce a hologram of an object from electromagnetic radiation, such as incoherent light, received from the object. The electromagnetic radiation is received by a receiving assembly and transformed into a plurality of co-linear co-propagating beams with different focal distances. The interference of the plurality of beams is enabled by projecting components of each beam along a common polarization direction. The interference patterns thus formed are recorded and then processed to form the hologram of the object.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.