A microfluidic system includes a hydrophobic fluidic platform and a heater. The platform includes a plurality of electrode cells operably connected to a voltage source and a controller. The heater is configured to fuse first and second vesicles. The first and second vesicles encapsulate first and second DNA precursors, respectively. The fusing combines the first and second DNA precursors. In another embodiment, a microfluidic system includes a fluidic platform including a plurality of electrode cells, a vesicle mover, and a reaction facilitator. The vesicle mover is configured to move first and second vesicles to a selected cell of the plurality of electrode cells. The reaction facilitator is operably connected to the selected cell. A method includes providing a fluidic platform comprising a plurality of cells; moving first and second vesicles encapsulating first and second reagents, respectively, to a first cell; and fusing the first and second vesicles.

Synthetic molecular tags are placed on an item at various points in a supply chain to create a molecular record of movement through the supply chain. Associations between each unique synthetic molecular tag and individual locations in the supply chain are stored in an electronic record which may be maintained in the cloud. The synthetic molecular tags are collected from the item and sequenced to determine movement of the item through the supply chain by reference to the electronic record. The synthetic molecular tags can be used for identifying recalled items based on locations in the supply chain associated with a recall. The synthetic molecular tags may be polynucleotides such as deoxyribose nucleic acid (DNA). The item may be any type of item including food.

Synthetic molecular tags are placed on an item at various points in a supply chain to create a molecular record of movement through the supply chain. Associations between each unique synthetic molecular tag and individual locations in the supply chain are stored in an electronic record which may be maintained in the cloud. The synthetic molecular tags are collected from the item and sequenced to determine movement of the item through the supply chain by reference to the electronic record. The synthetic molecular tags can be used for identifying recalled items based on locations in the supply chain associated with a recall. The synthetic molecular tags may be polynucleotides such as deoxyribose nucleic acid (DNA). The item may be any type of item including food.

There may be provided method for estimating an information unit represented by a cluster of traces that are noisy copies of a synthesized strand, the method may include estimating the information unit by applying processing operations on r-tuples related to the traces, wherein r is smaller than a number (t) of traces of the cluster; wherein processing operations applied on at least some of the r-tuples comprise calculating a length of a shortest common supersequences (SCS) of the r-tuples.

A first string, having a first string value, that is associated with a sample set of material is received, wherein a second string, having a complementary value relative to the first string value, is also associated with the sample set of material. A determinative hash is generated using the first string value and a symmetric generator polynomial. A second hash, corresponding to the second string, is generated directly from the determinative hash. A canonized hash is generated using the determinative hash and the second hash. It is determined whether at least one of the first string or the second string is stored in string storage that is configured to store a plurality of strings, including by searching a hash table for the canonized hash; in the event it is determined that at least one of the first string or the second string is not stored in the string storage, at least one of the first string or the second string is stored in the string storage.

A first string, having a first string value, that is associated with a sample set of material is received, wherein a second string, having a complementary value relative to the first string value, is also associated with the sample set of material. A determinative hash is generated using the first string value and a symmetric generator polynomial. A second hash, corresponding to the second string, is generated directly from the determinative hash. A canonized hash is generated using the determinative hash and the second hash. It is determined whether at least one of the first string or the second string is stored in string storage that is configured to store a plurality of strings, including by searching a hash table for the canonized hash; in the event it is determined that at least one of the first string or the second string is not stored in the string storage, at least one of the first string or the second string is stored in the string storage.

Methods and systems for sequence-controlled polymer encoding, decoding, and storage are provided. In various embodiments, input data is encoded into one or more sequence controlled polymer, wherein encoding the input data comprises applying an error-correction code. The one or more sequence-controlled polymer are synthesized. The synthesized one or more sequence-controlled polymer are encapsulated in a plurality of particles. The plurality of particles are embedded into a feedstock.

RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.

A method for asymmetric encryption based on a gene chip includes the steps of (a) obtaining original information in text or image or other form and converting the same into a binary code, and (b) preprocessing the binary code to obtain a binary matrix. In (c), an encryption key is obtained, the encryption key comprising a gene expression solution. In (d), the gene expression solution is placed on a gene chip according to an arrangement and correspondence of the binary matrix.

A method and system learn functions to be associated with data fields of forms to be incorporated into an electronic document preparation system. The functions are essentially sets of operations required to calculate the data field. The method and system receive form data related to a data field that expects data values resulting from performing specific operations. The method and system utilize machine learning and training set data to generate, test, and evaluate candidate functions to determine acceptable functions.