A direct structured illumination microscopy (dSIM) reconstruction method is provided. First, a time domain modulation signal is extracted through a wavelet. Then, an incoherent signal is converted into a coherent signal. Next, an accumulation amount at each pixel is calculated. Finally, a super-resolution image is generated by using a correlation between signals at different spatial positions. An autocorrelation algorithm of dSIM is insensitive to an error of a reconstruction parameter. dSIM bypasses a complex frequency domain operation in structured illumination microscopy (SIM) image reconstruction, and prevents an artifact caused by the parameter error in the frequency domain operation. The dSIM algorithm has high adaptability and can be used in laboratory SIM, nonlinear SIM imaging systems, or commercial systems.

Methods for evaluating micrometastases in a tissue region of a subject are described. The methods include administering to the subject a detectably effective amount of a tumor-targeted photoactivatable immunoconjugate; allowing a sufficient amount of time for the tumor-targeted photoactivatable immunoconjugate to enter micrometastases in the tissue region; illuminating the tumor-targeted photoactivatable immunoconjugate; obtaining an image of the tissue region of the subject using a fluorescent imaging device, and evaluating the micrometastases in the tissue region by conducting algorithmic analysis of the image. Methods of treating micrometastases in a tissue region of a subject are also described.

A method and a device are useful for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid (DNA) using Crithidia luciliae cells by fluorescence microscopy and by digital image processing.

Offset illumination using multiple emitters in a fluorescence imaging system is described. A system includes an emitter for emitting pulses of electromagnetic radiation and an image sensor comprising a pixel array for sensing reflected electromagnetic radiation. The emitter comprises a first emitter and a second emitter for emitting different wavelengths of electromagnetic radiation. The system is such that at least a portion of the pulses of electromagnetic radiation emitted by the emitter comprises electromagnetic radiation having a wavelength from about 770 nm to about 790 nm.

A system for displaying medical imaging data comprising one or more data inputs, one or more processors, and one or more displays, wherein the one or more data inputs are configured for receiving first image data generated by a first medical imaging device, wherein the first image data comprises a field of view (FOV) portion and a non-FOV portion, and the one or more processors are configured for identifying the non-FOV portion of the first image data and generating cropped first image data by removing at least a portion of the non-FOV portion of the first image data, and transmitting the cropped first image data for display in a first portion of the display and additional information for display in a second portion of the display.

An inspection apparatus is an inspection apparatus includes an excitation light source that generates excitation light to irradiate the object, a dichroic mirror that separates fluorescence from the sample by transmitting or reflecting the fluorescence according to a wavelength, a camera that images fluorescence reflected by the dichroic mirror, a camera that images fluorescence transmitted through the dichroic mirror, and a control apparatus that derives color irregularity information of a light-emitting element based on a first fluorescence image acquired by the camera and a second fluorescence image acquired by the camera, and an edge shift width corresponding to a width of a wavelength band in which transmittance and reflectance change according to a change in wavelength in the dichroic mirror is wider than a full width at half maximum of a normal fluorescence spectrum of the light-emitting element.

In one embodiment, a method for generating a three-dimensional (3D) anatomical map, including applying a trained artificial neural network to (a) a set of two-dimensional (2D) fluoroscopic images of a body part of a living subject, and (b) respective first 3D coordinates of the set of 2D fluoroscopic images, yielding second 3D coordinates of the 3D anatomical map, and rendering to a display the 3D anatomical map responsively to the second 3D coordinates.

A surgical guidance system has two cameras to provide stereo image stream of a surgical field; and a stereo viewer. The system has a 3D surface extraction module that generates a first 3D model of the surgical field from the stereo image streams; a registration module for co-registering annotating data with the first 3D model; and a stereo image enhancer for graphically overlaying at least part of the annotating data onto the stereo image stream to form an enhanced stereo image stream for display, where the enhanced stereo stream enhances a surgeon's perception of the surgical field. The registration module has an alignment refiner to adjust registration of the annotating data with the 3D model based upon matching of features within the 3D model and features within the annotating data; and in an embodiment, a deformation modeler to deform the annotating data based upon a determined tissue deformation.

There is provided a computer implemented method for generating a three dimensional (3D) image based of fluorescent illumination, comprising: receiving in parallel by each of at least three imaging sensors positioned at a respective parallax towards an object having a plurality of regions with fluorescent illumination therein, a respective sequence of a plurality of images including fluorescent illumination of the plurality of regions, each of the plurality of images separated by an interval of time; analyzing the respective sequences, to create a volume-dataset indicative of the depth of each respective region of the plurality of regions; and generating a 3D image according to the volume-dataset.

Methods and systems for digitally reconstructing a patient tissue sample are described herein. In one embodiment, the method may include projecting a first structured light pattern onto the patient tissue sample, receiving a first reflection of the first structured light pattern from the patient tissue sample, and reconstructing the patient tissue sample based on the first reflection and the projected first structured light pattern. In another embodiment, the system may include a projector adapted or configured to project the first structured light onto the patient tissue sample, a charge-coupled device (CCD) adapted or configured to receive the first reflection from the patient tissue sample, and a reconstruction device adapted or configured to reconstruct the patient tissue sample based on the first reflection and the projected first structured light pattern.