Methods for determining a value of an intrinsic geometrical parameter of a geometrical feature characterizing a physical object, and for classifying a scene into at least one geometrical shape, each geometrical shape modeling a luminous object. A singular light distribution characterized by a first wavelength and a position of singularity is projected onto the physical object. Light excited by the singular light distribution that has interacted with the geometrical feature and that impinges upon a detector is detected and a return energy distribution is identified and quantified at one or more positions. A deep learning or neural network layer may be employed, using the detected light as direct input of the neural network layer, adapted to classify the scene, as a plurality of shapes, static or dynamic, the shapes being part of a set of shapes predetermined or acquired by learning.

A system for manufacturing defect classification is presented. The system includes a first neural network receiving a first data as input and generating a first output, a second neural network receiving a second data as input and generating a second output, wherein first neural network and the second neural network are trained independently from each other, and a fusion neural network receiving the first output and the second output and generating a classification. The first data and the second data do not have to be aligned. Hence, the system and method of this disclosure allows various type of data that are collected during manufacturing to be used in defect classification.

A system for manufacturing defect classification is presented. The system includes a first neural network receiving a first data as input and generating a first output, a second neural network receiving a second data as input and generating a second output, wherein first neural network and the second neural network are trained independently from each other, and a fusion neural network receiving the first output and the second output and generating a classification. The first data and the second data do not have to be aligned. Hence, the system and method of this disclosure allows various type of data that are collected during manufacturing to be used in defect classification.

The technology disclosed relates to equalizer-based intensity correction for base calling. In particular, the technology disclosed relates to accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters, selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio, applying the pixel coefficients to intensity values of the pixels in the image to produce an output, and base calling the target cluster based on the output.

A classification training method for training classifiers adapted to identify specific mutations associated with different cancer including identifying driver mutations. First cells from mutation cell lines derived from conditions having the number of driver mutations are acquired and 3D image feature data from the number of first cells is identified. 3D cell imaging data from the number of first cells and from other malignant cells is generated, where cell imaging data includes a number of first individual cell images. A second set of 3D cell imaging data is generated from a set of normal cells where the number of driver mutations are expected to occur, where the second set of cell imaging data includes second individual cell images. Supervised learning is conducted based on cell line status as ground truth to generate a classifier.

A microscope system for scanning a bone marrow aspirate (BMA) sample may include a scanning apparatus for scanning the BMA sample and a processor coupled to the scanning apparatus and a memory. The microscope system may obtain, using the scanning apparatus, scan data of the BMA sample and detect cells in the BMA sample from the scan data. The microscope system may also classify the detected cells into a plurality of cell types, and store, in the memory, cell data of the classified cells. The microscope system may include a display for presenting the cell data. Various other systems and methods are provided.

A microscope system for scanning a bone marrow aspirate (BMA) sample may include a scanning apparatus for scanning the BMA sample and a processor coupled to the scanning apparatus and a memory. The microscope system may obtain, using the scanning apparatus, scan data of the BMA sample and detect cells in the BMA sample from the scan data. The microscope system may also classify the detected cells into a plurality of cell types, and store, in the memory, cell data of the classified cells. The microscope system may include a display for presenting the cell data. Various other systems and methods are provided.

Examples of the disclosure describe systems and methods for identifying, quantifying, and/or characterizing plant stomata. In an example method, a first set of two or more images of a plant leaf representing two or more focal distances is captured via an optical sensor. A reference focal distance is determined based on the first set of images. A second set of two or more images of the plant leaf is captured via the optical sensor, including at least one image captured at a focal distance less than the reference focal distance, and at least one image captured at a focal distance greater than the reference focal distance. A composite image is generated based on the second set of images. The composite image is provided to a trainable feature detector in order to determine a number, density, and/or distribution of stomata in the composite image.

In example implementations, an apparatus is provided. The apparatus includes a channel, a camera, and a processor. The channel contains a fluid and an object. The fluid is to move the object through the channel. The camera system is to capture video images of the object in the channel. The processor is to track movement of the object in the channel via the video images based on known flow dynamics of the channel.

Various techniques are provided for performing automated full-cell segmentation and labeling in immunofluorescent microscopy. These techniques perform membrane segmentation and nuclear seed detection separate and independently from each other, then combine their results to identify cell boundaries. Some embodiments use texture- and kernel-based image processing to perform the method. In some embodiments, the method for obtaining membrane features disclosed herein can be used in conjunction with or separate from the nuclear features. The results can be used for a variety of purposes, including whole-area cell segmentation in fluorescence-based tissue imaging.