Soil penetrometers capable of measuring soil reflectance along the direction of insertion of the penetrometer are provided. The penetrometer can house an array of sensors, such as, for example, a Vis-NIR reflectance sensor, a load cell, a displacement sensor, and a moisture sensor. The reflectance data collected using the penetrometer can allow the interpretation and quantification of soil constituents and contaminants at high vertical resolution, such as 3 cm or more.

A brightness colorimeter having a measurement error caused by linearly polarized light, which is corrected, includes: a lens module to which light irradiated from one side is input; a polarization conversion module configured to penetrate the light input through the lens module to convert polarization characteristics; a spectral module provided in one unit block to reflect and penetrate the light input through the polarization conversion module so as to branch the light in different three directions; filter modules arranged on progress paths of the light branched in different three direction through the spectral module to penetrate monochromatic light beams having specific spectra among the light branched in the three directions; and measurement modules arranged to correspond to exit angles of the monochromatic light beams penetrated through the filter modules, to measure at least one of a brightness, a colorimeter and a defect obtained by the monochromatic light beams.

A microplate reader simultaneously obtains Raman measurements from samples contained in non-adjacent wells. At least two Raman probes are positioned perpendicularly above or below the microplate to simultaneously acquire Raman spectra data of the non-adjacent liquid samples. Each probe is coupled to a laser and a spectrometer and includes a lens focusing laser light within the sample and collecting light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor (sCMOS or CCD) to image light from multiple probes simultaneously, spaced from one another to reduce crosstalk. A positioner moves the microplate plate or probes to acquire data from a different subset of non-adjacent samples, and may also vary laser focus within wells during data acquisition. Multiple fluorescence probes may simultaneously acquire fluorescence data from the same samples, or non-adjacent samples. Probes may be fiber-coupled and positioned within a reaction chamber of a liquid handling system.

Disclosed herein a spectral device with enhanced stability of optical sensor and an operating method of the device. According to an embodiment of the present disclosure, there is provided a spectral device including: a light splitter configured to split an incident light into a reference light and a signal light; at least one beam shutter configured to perform control for selectively outputting at least one of the reference light and the signal light and for blocking the two signals together; and a controller configured to provide an absorption property of a bio-material by comparatively quantizing an intensity of the reference light and an intensity of the signal light, which are received into a sensor through the beam shutter.

An imaging assembly of a spectral imaging ellipsometer includes an analyzer configured to polarize reflected light reflected from a sample surface, an imaging mirror optical system disposed on an optical path of the reflected light passing through the analyzer and including a first mirror having a concave surface and a second mirror having a convex surface, and a light detector configured to receive light passing through the imaging mirror optical system to collect spectral data. The reflected light is firstly reflected by the first mirror, the firstly reflected light is secondarily reflected by the second mirror and travels toward the first mirror again, and then thirdly reflected by the first mirror to be imaged on a light receiving surface of the light detector.

Disclosed are hyperspectral/multiple spectral imaging methods and devices. A method obtains first and second spectral image datasets of a region of interest (ROI). The first spectral image dataset is characterized by a first spectral range and the second spectral image dataset is characterized by a second spectral range. The method then performs a first spectral analysis on the first spectral image dataset and a second spectral analysis on the second spectral image dataset. Afterwards, the method determines one or more spectral signature(s) at a deeper layer of the ROI.

Provided is a spectral imaging apparatus. The spectral imaging apparatus includes: an optical filter including a plurality of band filter units having different center wavelengths; a sensing device configured to receive light passing through the optical filter; an imaging lens array including a plurality of lens units which respectively correspond to the plurality of band filter units and each implement imaging on the sensing device; and a transparent substrate which is apart from the sensing device. At least one of the optical filter and the imaging lens array is provided on the transparent substrate.

Aspects of the disclosure relate to an integrated spectral unit including a micro-electro-mechanical systems (MEMS) interferometer fabricated within a first substrate and a light redirecting structure integrated on a second substrate, where the second substrate is coupled to the first substrate. The light redirecting structure includes at least one mirror for receiving an input light beam propagating in an out-of-plane direction with respect to the first substrate and redirecting the input light beam to an in-plane direction with respect to the first substrate towards the MEMS interferometer.

An imaging system uses a dynamically varying coded mask, such as a spatial light modulator (SLM), to time-encode multiple degrees of freedom of a light field in parallel and a detector and processor to decode the encoded information. The encoded information may be decoded at the pixel level (e.g., with independently modulated counters in each pixel), on a read-out integrated circuit coupled to the detector, or on a circuit external to the detector. For example, the SLM, detector, and processor may create modulation sequences representing a system of linear equations where the variables represent a degree of freedom of the light field that is being sensed. If the number of equations and variables form a fully determined or overdetermined system of linear equations, the system of linear equations' solution can be determined through a matrix inverse. Otherwise, a solution can be determined with compressed sensing reconstruction techniques with the constraint that the signal is sparse in the frequency domain.

The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly includes a light source to emit light signals; a light detector to detect light signals; a coupler to optically couple the light source and the light detector to a waveguide tip; a monolithic substrate having a coupling side and a sensing side; and a lens between the waveguide tip and the monolithic substrate. The lens relays optical signals between the waveguide tip and the monolithic substrate.