A cavity-enhanced absorption spectroscopy instrument has an optical cavity with two or more cavity mirrors, one mirror of which having a hole or other aperture for injecting a light beam, and the same or another mirror of which being partially transmissive to allow exit of light to a detector. A spherical-spherical configuration with at least one astigmatic mirror or a spherical-cylindrical configuration where the spherical mirror could also be astigmatic prevents a reentrant condition wherein the injected beam would prematurely exit the cavity through the aperture. This combination substantially increases the number of passes of the injected beam through a sample volume for sensitive detection of chemical species even in less than ideal conditions including low power laser or LED sources, poor mirror reflectivity or detector noise at the wavelengths of interest, or cavity alignment issues such as vibration or temperature and pressure changes.

Disclosed are a fluorescence microscope light source apparatus and a fluorescence microscope capable of obtaining high-luminance light in a wavelength of 500 to 550 nm and having reduced background noise when a sample is observed. The fluorescence microscope light source apparatus to be installed in a fluorescence microscope including an illumination light bandpass filter includes: a laser diode that emits blue light as excitation light; a phosphor that converts the excitation light from the laser diode into illumination fluorescence with a wavelength region of 500 to 550 nm; an optical system that extracts the illumination fluorescence from the phosphor; a first condenser lens that condenses the excitation light onto the phosphor; a light guide body having one end face on which the illumination fluorescence is incident and the other end face from which the illumination fluorescence exits; and a second condenser lens that condenses the illumination fluorescence onto the one end face of the light guide body. A band-elimination filter that blocks or attenuates light, out of the illumination fluorescence, in a wavelength region including a transmission maximum wavelength and including no transmission minimum wavelength in the illumination light bandpass filter is provided on a light path of the illumination fluorescence.

A cavity-enhanced absorption spectroscopy instrument has an optical cavity with two or more cavity mirrors, one mirror of which having a hole or other aperture for injecting a light beam, and the same or another mirror of which being partially transmissive to allow exit of light to a detector. A spherical-spherical configuration with at least one astigmatic mirror or a spherical-cylindrical configuration where the spherical mirror could also be astigmatic prevents a reentrant condition wherein the injected beam would prematurely exit the cavity through the aperture. This combination substantially increases the number of passes of the injected beam through a sample volume for sensitive detection of chemical species even in less than ideal conditions including low power laser or LED sources, poor mirror reflectivity or detector noise at the wavelengths of interest, or cavity alignment issues such as vibration or temperature and pressure changes.

A biometric sensor that measures biometric information and a biometric analysis system including the biometric sensor are provided. The biometric sensor may include: a light source configured to emit light toward a region of interest of an object under examination, the light being diffused at the region of interest; a collimator that includes a though-hole and is configured to collimate the diffused light received from the region of interest; and a spectrometer configure to analyze the diffused light transmitted by the collimator.

A scanning microscope includes a scanning unit that causes irradiation light emitted by a light source to scan a sample, an optical system that guides the emitted light that has passed through the scanning unit to the sample, an isolation unit that includes a transmissive portion that enables the irradiation light to pass the transmissive portion and a reflective portion that reflects at least some of light that is included in emitted light generated from the sample as a result of the irradiation light being radiated to the sample and that has passed through the optical system and the scanning unit, and a detection unit that detects the emitted light that has passed through the isolation unit. The isolation unit is disposed in an optical path of the irradiation light between the light source and the scanning unit.

The disclosure relates to a lens assembly (1) for collimating incoming radiation (11,12) from an object (0) arranged along an optical axis, comprising: a first lens arranged (2) on the optical axis (13) of the lens assembly (1); a second lens (3) arranged on the optical axis of the lens assembly (1); a light filter (4) comprising a non-transparent sheet body with at least one hole (5); wherein the light filter (4) is arranged: between the first lens (2) and the second lens (3); in the focal point of the first lens (2) and the second lens (3); and so that the at least one hole (5) is close to or at the optical axis (13) of the lens assembly (1). The disclosure further relates to an optical spectrometer (7) for spectrally resolved two-dimensional imaging of an object (0) and a method for spectrally resolved two-dimensional imaging of an object (0).

A fiber optic probe assembly is provided. The probe comprises a first optical system and a second optical system, a delivery light guide comprising one or more than one delivery optical fiber for transmitting excitation radiation from a radiation source disposed at a proximal end of the light guide to the first optical system. The first optical system comprising one or more than one first optical element for forming a substantially collimated illumination beam from the excitation radiation. An optically opaque tubular sleeve is fitted over the first optical system to optically isolate the first optical system and the delivery light guide from the second optical system. The second optical system comprising one or more than one second optical element for gathering optical radiation scattered from a sample and forming the optical radiation into a collection beam. A collection light guide comprising one or more than one collection optical fiber receives the collection beam and transmits the collection beam to an analyzer. The first and second optical systems are disposed within a housing so that an emission cone of the first optical system and an acceptance cone of the second optical system substantially overlap. A spectroscopic measurement system comprising the optic fiber probe is also provided.

Optical devices that combine imaging with spectral detection usually require bulky or expensive optics or are limited in the spectral measurements that can be made. The disclosed device uses a lens and mirror to provide two image planes: one for a pixelated detector and the other for an optical fiber assembly. The optical fiber assembly may be scanned over most or all of the field of view, or this may be achieved with a fixed fiber assembly and translatable mirror. Multiple fibers may be included in the assembly and multiple measurement or other devices may be connected to the remote ends of the optical fibers.

A method of manufacturing a Fabry-Perot interference filter includes a forming step of forming a first thinned region, a first mirror layer, a sacrificial layer, and a second mirror layer are formed on a first main surface of a wafer, and the first thinned region in which at least one of the first mirror layer, the sacrificial layer, and the second mirror layer is partially thinned along each of a plurality of lines is formed; a cutting step of cutting the wafer into a plurality of substrates along each of the plurality of lines by forming a modified region within the wafer along each of the plurality of lines through irradiation of a laser light, after the forming step; and a removing step of removing a portion from the sacrificial layer through etching, between the forming step and the cutting step or after the cutting step.

A system, robot and method to measure the color of an area of a sample. The system includes a light source to emit spatially coherent light that includes a broad spectrum of wavelengths; an optical arrangement to scan an area of the sample, part-by-part, with a collimated beam of said light; an optical spectrometer to receive scattered light and measure an optical spectrum for each part; and a computing device. The optical arrangement includes a collimator and/or is configured to preserve collimated said spatially coherent light. The system is configured for synchronizing the scanning of the area with the recording of the optical spectra for the area's parts, the recording of the optical spectrum of each part lasting an optical spectrum integration time equal to the duration of the scan of said part. The computing device determines color coordinates, computes and analyzes an overall optical spectrum, calculates XYZ Tristimulus values.