An endoscopic imaging system for use in a light deficient environment includes an imaging device having a tube, one or more image sensors, and a lens assembly including at least one optical elements that corresponds to the one or more image sensors. The endoscopic system includes a display for a user to visualize a scene and an image signal processing controller. The endoscopic system includes a light engine having an illumination source generating one or more pulses of electromagnetic radiation and a lumen transmitting one or more pulses of electromagnetic radiation to a distal tip of an endoscope.

A method for detecting and identifying a remote gas, the method comprising the steps of: receiving a light wave associated with the remote gas; coupling the light wave into a single mode fibre; transmitting the light wave via the single mode fibre into a filter comprising a fibre-based tunable cavity; modulating the cavity length of the filter transmission window to cause a detected modulated signal that is proportional to the spectral feature; and processing the signal using a lock-in amplifier capable of low-pass filtering and out-of-frequency noise rejection.

A method for detecting and identifying a remote gas, the method comprising the steps of: receiving a light wave associated with the remote gas; coupling the light wave into a single mode fibre; transmitting the light wave via the single mode fibre into a filter comprising a fibre-based tunable cavity; modulating the cavity length of the filter transmission window to cause a detected modulated signal that is proportional to the spectral feature; and processing the signal using a lock-in amplifier capable of low-pass filtering and out-of-frequency noise rejection.

Systems and methods for hyperspectral imaging are described. In one implementation, a hyperspectral imaging system includes a sample holder configured to hold a sample, an illumination system, and a detection system. The illumination system includes a light source configured to emit excitation light having one or more wavelengths, and a first set of optical elements that include a first spatial light modulator (SLM), at least one lens, and at least one dispersive element. The illumination system is configured to structure the excitation light into a predetermined two-dimensional pattern at a conjugate plane of a focal plane in the sample, spectrally disperse the structured excitation light in a first lateral direction, and illuminate the sample in an excitation pattern with the one or more wavelengths dispersed in the first lateral direction.

Systems and methods for confocal imaging are described. In one implementation, a confocal imaging system may include a light source configured to emit excitation light having one or more wavelengths, a sample holder configured to hold a sample, a two-dimensional (2-D) imaging device, a first set of optical elements, and a second set of optical elements. The first set of optical elements may include a first spatial light modulator (SLM) and at least one lens. The first set of optical elements may together be configured to collimate the excitation light, apply a predetermined phase modulation pattern to the collimated excitation light, and illuminate the sample in an excitation pattern.

An embodiment of a system for measuring trace gas concentration is described that comprises a laser absorption spectrometer configured to detect an absorbance measure from a trace gas, as well as a temperature value and a pressure value that correspond to an environment in a gas cell; and a computer having executable code stored thereon configured to perform a method comprising: receiving the absorbance value, the temperature value, and the pressure value; defining a fitting range associated with the trace gas; applying a curve fitting model in the fitting range to the absorbance value using the temperature value and the pressure value as model parameters; and producing a concentration measure of the trace gas.

A light radiating portion radiates light with wavelength λ1 having predetermined absorptivity for an object and light with wavelength λ2 having smaller absorptivity for the object than the wavelength λ1, to a target, so as to scan in 2-dimensional directions. A light receiving portion receives scattered lights reflected by the target based on light with wavelength λ1 and light with wavelength λ2. A measuring portion generates information used for detection of the object at the target, based on difference between the two scattered lights with wavelength λ1 and wavelength λ2 received by the light receiving portion. An output portion outputs whether or not the object is present at the target, by 2-dimensional area information, based on scanning by the light radiating portion and information generated by the measuring portion.

A light radiating portion radiates light with wavelength λ1 having predetermined absorptivity for an object and light with wavelength λ2 having smaller absorptivity for the object than the wavelength λ1, to a target, so as to scan in 2-dimensional directions. A light receiving portion receives scattered lights reflected by the target based on light with wavelength λ1 and light with wavelength λ2. A measuring portion generates information used for detection of the object at the target, based on difference between the two scattered lights with wavelength λ1 and wavelength λ2 received by the light receiving portion. An output portion outputs whether or not the object is present at the target, by 2-dimensional area information, based on scanning by the light radiating portion and information generated by the measuring portion.

A spectrometer includes an emitter that is configured to emit electromagnetic radiation, a sample area that is arranged at an outer face of the spectrometer, a modulation unit including an electrochromic material, an optical filter, an optical detector, an integrated circuit that has a main plane of extension, and an optical path for electromagnetic radiation emitted by the emitter towards the optical detector via the sample area, the modulation unit and the optical filter, wherein the electrochromic material is electrically connected with the integrated circuit, and the modulation unit is configured to modulate electromagnetic radiation temporally. Furthermore, a method for detecting electromagnetic radiation is provided.

Methods and apparatus for obtaining a vibrational circular dichroism (VCD) image using a discrete frequency infrared (DFIR) microscope are disclosed. The method includes generating a pulsed laser beam comprising a spectral frequency, which may be tunable; modulating the laser beam to generate circularly polarized light; illuminating a sample and collecting, and detecting an optical signal transmitted or transflected from the location of the sample. The detected signal is demodulated at, for example, both the pulse frequency and the sum or difference of the pulse frequency and the modulating frequency to obtain an intensity value that correspond to the absorbance, and a polarization-dependent value that corresponds to the VCD. Other configurations of the apparatus may be employed to measure VCB and VLD.