The present invention relates to a method for analyzing a biological sample on a single cell level by compartmentalizing said sample using a grid and performing an optimized combinatorial indexing protocol within cells of the compartmentalized tissue while deducing cell-specific information regarding the cell identity and activity in the spatial context within the sample.

A method of preparing a sample may include depositing an aqueous solution comprising copies of a primer into a layer of hydrophobic liquid on a substrate with a thermal inkjet device. A sample may include: a substrate; a layer of hydrophobic liquid on the substrate, the layer of hydrophobic liquid comprising a plurality of droplets of aqueous solution distributed in the layer, wherein the plurality of droplets contain: primers; a polymerase enzyme; deoxynucleotide triphosphates (dNTPs); and a target sequence for replication; and a cover, the cover contacting and covering the layer of hydrophobic liquid.

A method includes applying a liquid cover to a microscopic sample to obtain a processed microscopic sample, generating at least one marker for a part of the processed microscopic sample using an imaging system, removing at least a part of the liquid cover from the processed microscopic sample to obtain an uncovered microscopic sample, and extracting a part of the uncovered microscopic sample, based on the at least one marker, to obtain an extracted part.

The present disclosure provides methods for automated slide assessments made in conjunction with digital image-based microscopy. Automated methods of acquiring patient information and specimen information from prepared slides, and digitally linking such information into patient-tagged specimen data, are provided. Also provided are methods that include automatically identifying an optimal area for morphological assessment of a blood smear on a hematological slide, including methods for triggering the analysis of such an area, e.g., using an automated digital image-based hematology system. The present disclosure also provides devices, systems and computer readable media for use in performing processes of the herein described methods.

Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.

The present invention relates to the methods, devices, and systems that make bio/chemical sensing (including, not limited to, immunoassay, nucleic assay, electrolyte analysis, etc.) faster, more sensitive, less steps, easy to perform, smaller amount of samples required, less or reduced (or no) needs for professional assistance, and/or lower cost, than many current sensing methods and devices. The present invention also allows a test performed by a smartphone.

A method is disclosed for demonstrating the cleansing efficacy of a personal care product or a component thereof, the method comprising: (i) selecting a first portion of a porous article capable of allowing a gas to pass through its pores, wherein the porous article is connected to a source of said gas and immersed in a liquid while the source releases said gas which flows out of said pores to generate gas bubbles; (ii) treating the first portion of the porous article with contaminants; (iii) treating the first portion of the porous article with the personal care product or the component thereof, wherein a second portion of the porous article is selected in step (i); the second portion is also treated with contaminants in step (ii); and the second portion is treated with a comparative or placebo product in step (iii); and wherein following step (iii) the method comprises a step (iv) of assessing a change of the treated first portion relative to untreated article and/or relative to the treated second portion, the change is the amount of gas bubbles released from the porous article.

The present application relates generally to image tiling, including but not limited to systems and methods of fast whole slide tissue tiling. A computing system may identify a first image of a first dimension from which to select one or more tiles. The computing system may perform a reduction operation on the first image to generate a second image of a second dimension. The computing system may apply a thresholding operation on the second image to identify a first set of pixels corresponding to the presence of the feature and a second set of pixels corresponding to the absence of the feature based on an intensity of each pixel in the second image. The computing system may select, from a plurality of tiles corresponding to the first image, a subset of tiles corresponding to the first set of pixels identified from the second image.

The present invention is related to correct the errors in instruments, operation, and others using intelligent monitoring structures and machine learning, and others.

The present invention relates to the methods, devices, and systems that make bio/chemical sensing (including, not limited to, immunoassay, nucleic assay, electrolyte analysis, etc.) faster, more sensitive, less steps, easy to perform, smaller amount of samples required, less or reduced (or no) needs for professional assistance, and/or lower cost, than many current sensing methods and devices. The present invention also allows a test performed by a smartphone.