A sensitive method for quantitating curcuminoids in biological and other samples is described wherein curcuminoids in the sample are derivatized to boron difluoride curcuminoid complexes then analyzed by liquid chromatography-mass spectroscopy.

A tissue grinding system includes a hollow collection tube defining an internal cavity including an opening and a lid corresponding to the opening, a pestle including a rod and a head extending from an end of the rod having a shape corresponding to the shape of the internal cavity, and at least one groove extending about an outer surface of the head of the pestle at an angle. The at least one groove extends a depth into the head of the pestle. The head of the pestle is configured to grind a substance within the internal cavity.

Method for quantifying the micronutrient profile of Moringa oleifera tree leaves (MOLs) using calibration free laser induced breakdown spectroscopy (CF-LIBS).

Disclosed herein are a sonic homogenizing module (100, 200, 300) and a biological sample preparation system (500) containing the same. The sonic homogenizing module (100, 200, 300) comprises a rod made of a magnetic material (120, 220, 320); a piezoelectric conductor (130, 230,330); a driver (140, 340); and a sleeve-coupling member (110, 210, 310) having a first portion defining a space (112, 212, 312) for coupling with a gripper module of a biological sample preparation system, and for accommodating the piezoelectric conductor and the driver therein; and a second portion having a conduit (114, 214, 314) for receiving the rod therethrough; wherein the driver (140, 340) is electrically coupled with the piezoelectric conductor (130, 230,330) and is configured to drive the piezoelectric conductor (130, 230, 330) to generate a sonic vibration at a frequency of 100 KHz-1 MHz.

A bio-sample homogenizer with a direct drive motor generating reciprocal motion for sample disruption, following continuous rotation for centrifuge tissue sample dissociation for debris separation. The homogenizer device comprises: a motor, a plurality of sample containers, a frame work to mount the sample containers. The direct drive motor generates constant acceleration/deceleration to drive the platform for producing a horizon reciprocal motion. A plurality of holding slots mounted on the framework for holding the plurality of sample containers containing with beads and tissue sample. The beads in the sample containers cause a blending action on the tissue sample for fully dissociation of the sample. Following continuous rotation generating centrifuge force, the finely dissociated solution is separated from the debris.

A method for determining the content of menthol in a traditional Chinese medicine composition. The traditional Chinese medicine composition consists of the following medicinal materials: Fructus Forsythia, Flos Lonicerae, Radix Isatidis, Semen Armeniacae Amarum, menthol, Herba Houttuyniae, rheum, Herba Pogostemonis, Rhizoma Dryopteris Crassirhizomae, Rhodiola rosea L., Herba Ephedrae, Radix Glycyrrhizae and gypsum. In the method for determining the content, the content of the menthol in the composition is determined by gas chromatography to effectively control the content of menthol in the composition, and the method can save energy and reduce the costs for analysis.

This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.

A method for measuring a resistivity of a silicon single crystal by a four-point probe method including: a first grinding step of grinding at a surface of the silicon single crystal on which the resistivity is measured; a cleaning step of cleaning the silicon single crystal subjected to the first grinding step; a donor-annihilation heat treatment step of heat-treating the silicon single crystal subjected to the cleaning step; and a second grinding step of grinding at least the surface of the silicon single crystal subjected to the donor-annihilation heat treatment step on which the resistivity is to be measured, where the resistivity of the silicon single crystal is measured by the four-point probe method after performing the second grinding step. This provides a method for measuring a resistivity of a silicon single crystal by which stable measurement is possible over a long period of time after a donor-annihilation heat treatment.

The invention provides an intelligent quantitative microscopic identification system for whole rock polished sections, which can greatly improve the collection efficiency of whole rock polished sections by adopting a microscopic collecting apparatus. In a preferable technical solution, the microscopic collecting apparatus is combined with the production line for the automatic preparation of whole rock polished sections to form an integrated system, which realizes an automatic solution from preparation to collection, therefore further improves the production and collection efficiency of whole rock polished sections, and which can cope with production, image collection and automatic scanning and splicing of mass whole rock polished sections, so it greatly improves the collection efficiency of the microscopic images of organic components for whole rock polished sections. People can collect 500 to 1000 sample pieces of whole rock polished sections in 12 hours by adopting the solution of the invention.

Disclosed is a characterization method of closed pores and connectivity of coal measure composite reservoirs, including collecting samples of coal seams and shales reservoirs, carrying out low-field NMR experiments and NMR freeze-thaw experiments on plunger samples and crushed samples with different particle sizes to obtain cumulative pore volume distribution and differential pore size distribution of the crushed samples, comparing crushed samples with plunger samples for optimal crushed particle sizes, and preliminarily determining a distribution range of closed pores; carrying out SAXS experiments on crushed samples to obtain size distribution and volume of total pores of 1-100 nanometers; calculating pore volume of total pores and closed pore volume in composite reservoirs by low-field NMR experiments results; carrying out non-steady overburden permeability experiments and variable factors on plunger samples of coal seams, shales and tight sandstone to characterize the connectivity under influence of pores development and lithologic combinations.