This disclosure is directed to solutions and methods of using the solutions. A first solution includes a polymer, a polyol, and a buffer. A second solution includes a polymer, a polyol, and an alcohol.

The present invention relates to a hybrid nanodot made by a process comprising a step of reacting a mixture of an amino acid and a polymer selected from polycationic polymers or any copolymers or derivatives of these polymers under hydrothermal reaction conditions. The present invention also relates to a process for synthesizing said hybrid nanodots.

A smear preparing apparatus of an embodiment includes: a transportation part that holds and transports a microscope slide; a housing defining a room therein, the housing including: an insertion opening through which the microscope slide transported by the transportation part is to be inserted, and a feed opening through which air is to be fed; and a blower configured to feed the air through the feed opening to the microscope slide housed in the housing. The housing further includes a discharge opening through which the air fed from the blower through the feed opening is discharged.

A computer-implemented method (530), system (100, 201) and computer readable medium for generating a slide-free histological image in the form of a reconstructed ultraviolet-based autofluorescence microscopy (UV-AutoM) image from a plurality of speckle-illuminated low-resolution images. A computer implemented method (1150), system (100, 201) and computer readable medium for generating a slide-free histological image in the form of a pseudo-hematoxylin and cosin (H&E) stained image (1120) from a grayscale input image such as a UV-AutoM image or a ultraviolet-based photoacoustic microscopy (UV-PAM) image (1110).

A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

The disclosure provides antibodies that specifically bind human IDO and methods of use thereof. In some aspects, the disclosure is directed to methods of detecting IDO in a biological sample comprising contacting the biological sample with an antibody described herein.

Milling with ultraviolet excitation (MUVE) realizes high-throughput multiplex imaging of large three-dimensional samples. The instrumentation may comprise a UV-source attachment, precision stage attachment, and/or a blade assembly, and the instrumentation may overcome several constraints inherent to current state-of-the-art three-dimensional microscopy. MUVE offers throughput that is orders of magnitude faster than other technology by collecting a two-dimensional array of pixels simultaneously. The proposed instrumentation also utilizes serial ablation and provides the opportunity for true whole-organ imaging at microscopic resolution.

The present disclosure relates to a diagnostic method and a device performing the same. According to an aspect of the present disclosure, a diagnostic device is a diagnostic device that uses a test kit including a specimen plate having a specimen region in which a specimen is smeared and a patch plate configured to store a contact-type patch, which comes into contact with the specimen to stain the specimen, and the diagnostic device includes a body having a loading region in which the test kit is placed, a moving unit configured to move the patch plate and the specimen plate of the test kit relative to each other so that the specimen placed in the test kit is smeared in the specimen region, and a contact unit configured to move a structure of the test kit such that the contact-type patch comes into contact with the smeared specimen so that the smeared specimen is stained.

The invention relates to a method for rapidly determining the susceptibility of a microorganism to an antimicrobial agent comprising the steps: a) contacting a first sample containing the microorganism with a first growth medium so as to form a first mixture, wherein the first growth medium is selected to enable the microorganism to proliferate and/or encourage the microorganism cell cycle to commence proliferation; b) contacting a second sample containing the microorganism with a second growth medium so as to form a second mixture, wherein the second growth medium is substantially the same as the first growth medium but further comprises a first antimicrobial agent which may inhibit or slow the proliferation of the microorganism; c) incubating the first and second mixtures, for 30 minutes or less, under conditions suitable to enable or encourage proliferation of the microorganism; d) passing the first and second mixture, or portion thereof, through a flow cytometer in order to assess one or more biochemical and/or biophysical parameters of the microorganisms in both mixtures; and e) comparing the parameters of the microorganisms in the first mixture with that of the second mixture, after incubation, in order to detect whether the first antimicrobial agent inhibits or slows the proliferation of the microorganism so as to determine the susceptibility of a microorganism to said agent. The method is particularly suited for identifying the which antimicrobial agents would be suitable for the treatment of microbial infections, such as Urinary Tract Infections (UTIs)

This disclosure provides a method for analyzing peripheral blood leukocytes. In some embodiments, method may comprise: (a) labeling peripheral blood leukocytes isolated from a patient that has or is suspected of having inflammatory bowel disease (IBD) with a panel of distinguishably-labeled antibodies and (b) analyzing binding of the antibodies to the peripheral blood leukocytes. Kits for performing the method are also provided. Results from the analysis can be used to make a diagnosis of Crohn's disease or ulcerative colitis.