Disclosed are an improved tissue processor and a method of tissue processing performed by the tissue processor. The improved method for tissue processing or the tissue processor is capable of supporting the xylene-free treatment and effectively removing the substitute of xylene (such as isopropanol, IPA), without addition of further protective chemical reagent to remove the isopropanol, thus simplifying the tissue processing method and saving cost significantly.

Disclosed herein are potency assays for a gene therapy treatment for sickle cell disease. Also disclosed herein are methods for measuring relative potency of a drug product used for the treatment of sickle cell disease.

Devices and systems are provided herein relating to a novel and rapid assay for tissue staining. Methods for using the devices and systems for analyzing tissue samples are also disclosed.

The present invention relates to a composition for clearing a biotissue and a method for clearing a biotissue using thereof.

A method of detecting the presence of a prostate cancer in a human subject comprising the steps of (a) obtaining a histologically normal prostate tissue sample from the patient and (b) quantifying the epithelial thickness or gland lumen roundness of the tissue, wherein an increase in epithelial thickness or a decrease in gland lumen roundness indicates the presence of prostate cancer or a prostate cancer field defect.

Disclosed herein are compositions for fixing tissue for cytologic, histomorphologic, and/or molecular analysis (e.g., DNA, RNA, and/or protein analysis). In some embodiments, the fixatives are aldehyde-free fixatives, for example, formaldehyde- or formalin-free fixatives. Particular disclosed compositions include buffered ethanol. The buffer is a phosphate buffer or phosphate buffered saline (PBS) in some examples. In further embodiments, the fixative includes additional components, such as glycerol and/or acetic acid.

A TEM electromechanical in-situ testing method of one-dimensional materials is provided. A multi-function sample stage which can compress, buckle and bend samples is designed and manufactured. A carbon film on a TEM grid of Cu is eliminated, and the TEM grid of Cu is cut in half through the center of the circle. The samples are dispersed ultrasonically in alcohol and dropped on the edge of the semicircular grid of Cu with a pipette. A single sample is fixed on the edge of a substrate of the sample stage with conductive silver epoxy by using a micromechanical device under an optical microscope, and conductive silver paint is applied to the surface of the substrate of the sample stage; and an electromechanical in-situ testing is conducted in a TEM. This provides a simple and efficient sample preparation and testing method for a TEM electromechanical in-situ observing experiment.

An apparatus and method for producing a coated analytic substrate using a compact and portable automated instrument located in the laboratory setting at the point of use which can consistently produce one or a plurality of coated analytic substrates “on demand” for using the analytic substrate immediately after coating, preferably without a step of rinsing the coated analytic substrate before use. The apparatus preferably uses applicator cartridges having a reservoir containing the coating compositions used to form the coatings. Preferably the cartridges are removable and interchangeable to facilitate the production of individual analytic substrates having different coatings or different coating patterns. These coated analytic substrates have superior specimen adhesion characteristics due to the improved quality of the coatings applied by the coating apparatus and due to the quickness with which the coated analytic substrates can be used in the lab after production.

Methods of imaging biomolecules from a tissue sample are described, including methods for preparing a tissue sample for imaging using expansion microscopy, while achieving ultrahigh effective imaging resolution.

The invention provides methods for preparing lymphocyte samples for flow cytometric analysis. The method includes: centrifuging anticoagulant blood samples to obtain blood cell precipitate, diluting the blood cell precipitate with PBS solution, then adding into a centrifuge tube containing lymphocyte separation solution, centrifuging, discarding the plasma to obtain buffy coat cells; then adding the buffy coat cells into a centrifuge tube, adding PBS solution, centrifuging, discarding the supernatant, adding PBS solution to adjust the concentration of cells, adding the cell solution into a flow tube; then adding antibody, mixing, incubating without light and centrifuging, discarding the supernatant and resuspending the cells in pre-cooled PBS-EDTA solution. The samples obtained enriches and purifies lymphocytes, fully preserves the activity of lymphocytes, removes granulocytes, red blood cells, platelets or other blood components, and the subpopulations to be detected are purified, the analysis and detection are more accurate, and costs for analyzing antibodies and reagents are saved.