A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

An inline filter housing with a biodegradable, hydrophilic material that operates in conjunction with a field sampling apparatus to both concentrate field sampled environmental DNA particles from water samples and to automatically preserve the captured DNA via desiccation, thus avoiding filter membrane transfer steps, chemicals or cold storage preservation requirements. The hydrophilic filter housing is capable of rapidly preserving the field sampled environmental DNA captured on the filter membrane at ambient field temperatures.

Identification of infectious agents, in samples like blood, urine, mouthwash obtained from patients, is the most important tool for laboratory diagnosis of infectious diseases. Due to their technical nature, diagnostic tests can use only a small part of the sample obtained from the patient. For that reason, it is very important to concentrate infectious agents into a small volume of sample that will be used in diagnostic tests, to increase their sensitivity. Additionally, there may be substances that interfere by working of the diagnostic tests based on nucleic acid amplification like polymerase chain reaction (PCR). It is important to remove these substances so that this kind of diagnostic tests can work properly. This invention is a method of concentrating infectious agents in biological samples by using elastic polymer meshes. When added to liquid biological samples, these meshes remove water and small molecules from their environment, diminish the volume of the sample and thus enable concentrating the microorganisms Concentration of microorganism and removal of substances that inhibit the working of diagnostic methods, increase the sensitivity of these methods.

A platelet filtration membrane and its application for preparing platelets rich plasma and separating platelets from blood samples are disclosed. The platelet filtration membrane comprises a coating layer and a porous substrate. The coating layer composition comprises a first copolymer having a plurality of amide groups and a second copolymer having a plurality of carboxylic acid groups, and the porous substrate comprises PE, PP, PS, PET, PTFE, PVDF, ceramic or rayon. The coating layer is on surfaces of the porous substrate to form the platelet filtration membrane.

A concentrator includes a casing, a separation membrane that sections an inner space of the casing to form a flow path in the casing, a first supplier that supplies a first liquid from a first position of the casing to the flow path such that the first liquid flows along the separation membrane in a first direction, a second supplier that supplies a second liquid from a second position of the casing to the flow path such that the second liquid flows along the separation membrane in a second direction opposite to the first direction, and a third supplier that supplies a third liquid including a target component having a size that does not allow permeation of the target component through the separation membrane from a third position of the casing to the flow path.

A device including an ion-selective membrane arranged within an optical path of the device and coupled to a sample cell to interact with a fluid sample and thereby modify an optical response of the ion-selective membrane according to an ion concentration in the fluid sample, is provided. The device also includes an integrated computational element (ICE) arranged within the optical path, so that the illumination light optically interacts with the ICE and with the ion-selective membrane to provide a modified light that has a property indicative of the ion concentration in the fluid sample. A detector that receives the modified light provides an electrical signal proportional to the property of the modified light. A method and a system for using the above device are also provided.

A biological fluid separation device that is adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

The invention relates to a device (10) for extracting volatile species from a liquid (20) connected to an inlet of an analysis instrument, such as a mass spectrometer (MS). The device has a chamber (4), a membrane (5) forming a barrier for the liquid at zero differential pressure between the inside and the outside of the chamber, and allowing passage of the volatile species at zero differential pressure between the inside and the outside of the chamber. The device has an inlet capillary channel (3) to feed in a carrier gas and prevent back-diffusion from the chamber, and an outlet capillary channel (6) which provides a significant pressure reduction, e.g. from atmospheric pressure in the chamber (4) to near-vacuum suitable for an MS. The invention combines the best of two worlds, i.e. the fast time-response of a DEMS system and the high sensitivity of a MIMS system, since a differential pumping stage is not needed.

This disclosure relates to mesofluidic devices and methods for eluting and concentrating a plurality of nucleic acid molecules. The mesofluidic device includes a device frame having a bottom surface upon which is defined a first reservoir and the second reservoir. The first reservoir includes a first electrode, and the second reservoir includes a second electrode. The first and second electrodes are configured for electrical connection. The mesofluidic device includes an elongated channel extending between the first reservoir and the second reservoir. The mesofluidic device includes a first slot having a first slot width. The first slot is configured to receive an insert. The first slot intersects the elongated channel. The mesofluidic device includes a second slot having a second slot width. The second slot is configured to receive a separation material having a first porosity. The second slot intersects the elongated channel.