A water quality sampler includes a membrane receptacle and at least 7 sample membranes. The membrane receptacle comprises at least 7 membrane cavities disposed on a top surface of the membrane receptacle. Each membrane cavity is configured to hold a sample membrane and the sample membranes are each removably positioned within the membrane cavities and extend at least 1 cm out from the top surface of the membrane receptacle. A bottom of the membrane receptacle comprises a protruding lip that traces a bottom edge of the membrane receptacle in a downward direction, where the protruding lip encompasses a stacking chamber capable of receiving a top end of a second water quality sampler to allow stacking of multiple water quality samplers.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

Disclosed herein are systems, methods, and kits for collecting and storing a sample from a subject. A system can comprise a cartridge assembly for separation of the blood. The cartridge assembly can comprise a cartridge port configured to couple to a sample acquisition device that is usable to collect the blood from the subject. The cartridge assembly can comprise at least one blood separation membrane that is configured to separate plasma or serum from the blood. In some cases, the cartridge port can comprise a pathway that is configured to direct the blood to flow from the sample acquisition device, through the pathway, and towards the cartridge assembly. In some cases, a direction of flow of the blood through the at least one blood separation membrane can be different from a direction of flow of the blood through the pathway and towards the inner portion of the cartridge assembly.

The present disclosure provides, inter alia, systems and methods for the capture and retrieval of pathogens and/or other analytes of interest (e.g., aerosolized pathogens or other analytes).

Asymmetric depth filtration for isolation of EVs or other desired nanoparticles from a biological or other fluid with high yield and purity in a simple, cost-effective manner. Such a method includes passing the biological fluid through the asymmetric depth filter (e.g., in a single pass) where the fluid is introduced into the filter at an entry portion, where components of the biological fluid pass through the wider entry portion of the pores before advancing towards the narrower exit portion of the pores, wherein EVs in the fluid become reversibly entrapped within the wider portion of the pores, while similarly sized soft, low-density lipids and/or proteins are pushed more deeply into the filter, so that the reversibly entrapped EVs can be released by simply reversing the flow, while the similarly sized soft low-density lipids and/or proteins remain permanently entrapped within the pores of the filter.

A sampling device including an intake pump coupled with a volume controller to provide a known volume of samples such as liquid collected through a specialized hollow membrane filter. Once the known volume of sample is filtered the intake pump is turned off, an air pump turns on and removes residual or excess sample. The membrane filter is then preserved for extraction of DNA/RNA.

A system for separating and concentrating ionic compounds in a sample is provided herein. In some embodiments, a system includes at least one sample separation device, a flexible holder having a plurality of slots, wherein each slot is configured to have a sample separation device inserted therein, wherein the at least one sample separation device is inserted into a slot of the flexible holder; and an electrode for applying a voltage to the device, wherein the at least one sample separation device comprises a separation portion, a first storage portion, and a second storage portion, wherein the first and second storage portions are positioned on opposing sides of the separation portion, wherein the separation portion is folded at a predetermined interval to form two or more discrete base units.

A paper-based micro-concentrator includes a bearing substrate, a fluid reservoir unit, a filter paper, an external electric field, an ion exchange membrane and a magnet. The fluid reservoir unit includes a first buffer solution tank and a second buffer solution tank, which are interval disposed on the bearing substrate. The filter paper is disposed on the bearing substrate, and two ends of the filter paper are respectively placed in the first buffer solution tank and the second buffer solution tank. The external electric field includes a cathode and an anode, which are respectively placed in the first buffer solution tank and the second buffer solution tank. The ion exchange membrane is disposed on the filter paper and close to the first buffer solution tank. The magnet is movably disposed under the bearing substrate.

A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

A preprocessing apparatus uses preprocessing kits prepared for samples, respectively, sets the preprocessing kits in plural processing ports provided so as to correspond to preprocessing items, and executes a predetermined preprocessing item. A controller which controls operations of a carrying mechanism holding and carrying the preprocessing kits, and processing parts executing the preprocessing items in the respective processing ports, includes a processing-state control part, a random access part, and a preprocessing part. The random access part is configured to check availability of a preprocessing port corresponding to a preprocessing item to be executed on a sample contained in a preprocessing kit, and set the preprocessing kit in a processing port if the processing port is available as a processing port corresponding to the preprocessing item. The preprocessing part executes a corresponding preprocessing item on a sample in a preprocessing kit when the preprocessing kit is set in the processing port.