A hybrid gas sampling device can combine the functionality of both whole air and sorbent based samplers. The sampling device can be used for collecting light to very heavy organic compounds, for subsequent thermal desorption into a GC or GCMS for quantitative measurement. The sampling device isolates collected samples of gas phase matrices in a sample vessel, provided with sorbent elements from a removable sample extraction device. The sampling device is operated by drawing a vacuum on the chamber through the sample extraction device after sampling, and then completing the extraction of the heavier organic compounds using a static, diffusive extraction under vacuum to allow optimal deposition of the heavier compounds on the sorbent. The vacuum container is cooled to draw any excess water back into the container, thereby dehydrating attached sorbent element(s) in preparation for thermal desorption into a GC or GCMS, eliminating interferences in the MS analyzer.

A biological sample purification apparatus is described for purifying a protein from a cell, as well as methods of use of the purification apparatus, and systems comprising the same. The described apparatus comprises a housing comprising a top opening, a bottom opening, and a membrane positioned between said top opening and said bottom opening; and a purification media comprising diatomaceous earth and a resin, wherein the purification media is positioned between the membrane and the top opening; and wherein the purification media is optionally mixed and is substantially dry.

The present invention provides a lectin-magnetic carrier coupling complex for separating glycosylated exosomes from a clinical sample. The lectin-magnetic carrier coupling complex comprises a magnetic carrier and lectins coupled to the outer side of the magnetic carrier. The lectin-magnetic carrier coupling complex provided by the present invention may rapidly, accurately, and automatically separate glycosylated exosomes from a clinical sample with a high separation efficiency; and the separated exosomes are intact in morphology without rupturing or cracking, may be directly used for liquid detection of glycosylated exosomes, or directly used for immunology-related detection, or directly used for nucleotide sequence detection and analysis after extracting nucleic acids from the exosomes.

Methods of determining the presence or absence of fluorocarbon(s) on a substrate using X-ray Photoelectron Spectroscopy (XPS). A method may be used to determine the presence or absence of per- or polyfluoroalkyl substances PFASs. A method may use a porous polymer substrate. A method may use solid-phase extraction (SPE). A method may be used to determine the presence or absence of fluorocarbons in an aqueous sample. An aqueous sample may be a groundwater sample, wastewater sample, potable water sample, drinking water sample, or surface water sample. The limit of detection of fluorine in a method may be 0.05% F or less (for XPS analysis) and/or 20 ng or less on a substrate.

A surface-enhanced Raman scattering (SERS) detection method is provided for detecting a target analyte in a sample. The SERS detection method generally includes the steps of: (a). preparing an extract of the sample; (b). introducing the sample extract onto a SERS substrate, causing the target analyte to be absorbed in the SERS substrate; (c). introducing a volatile organic solvent onto the SERS substrate to have the target analyte of the sample extract dissolved and comes out of the SERS substrate; (d). irradiating the SERS substrate with light to evaporate the volatile organic solvent, leaving a more condensed target analyte on the SERS substrate; (e). irradiating the condensed target analyte with laser light to have the target analyte penetrate deeply into the SERS substrate; and (f). performing Raman measurement with a laser beam focusing on the SERS substrate to analyze the target analyte.

Reagents and methods for obtaining a metabolite solution comprising a mass spectrometry compatible volatile salt or volatile compound.

The subject invention provides materials, devices and methods for detecting, determining, monitoring and/or extracting trace metals such as cadmium, lead, copper, chromium, cobalt, nickel, zinc, manganese, mercury, and vanadium in the environmental, biological, pharmaceutical, and potable water samples. The subject invention also provides formulations and method for synthesizing the trace metal-extracting materials.

The invention relates to a system and micro monitor apparatus, a space-, time-, and cost-efficient device to concentrate, identify, and quantify organic compounds in gas environments. The invention further relates to a method centered on gas chromatography for identifying and quantifying organic compounds in gas environments, using air as the carrier gas, without the need for a compressed pre-bottled purified carrier gas.

The present disclosure provides a facile method for selective isolation of hyaluronan (HA) from fluids and tissues by adsorption to a solid matrix. The method involves the use of specific solvent conditions and solid phase media favoring binding and subsequent elution of HA ranging in size from about 10 kDa to several MDa, while allowing separation of HA from other biological polyanions such as nucleic acids, sulfated glycosaminoglycans, chondroitin, and heparosan.

A sample probe for sorptive sampling comprises an elongate body having a longitudinal axis defined along its length and a radial axis extending transverse to the longitudinal axis. A sorbent element formed of a sorbent material is secured to the body. The elongate body has an outer surface and a recess located along the length of the body that extends radially into the outer surface. The sorbent element is at least partially received within the recess of the body which mechanically locks the sorbent element to the body to prevent relative longitudinal movement of the sorbent element relative to the body.