Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.

A compensation bead comprising an amine functionalized bead modified covalently or non-covalently with a carboxyaryl porphyrin, a method for making the amine functionalized bead with the carboxyaryl porphyrin and a method of using the amine functionalized bead with the carboxyaryl porphyrin as a marker for the carboxyaryl porphyrin labeled cells and particles.

Methods, devices, apparatus, and systems are provided for image analysis. Methods of image analysis may include observation, measurement, and analysis of images of biological and other samples; devices, apparatus, and systems provided herein are useful for observation, measurement, and analysis of images of such samples. The methods, devices, apparatus, and systems disclosed herein provide advantages over other methods, devices, apparatus, and systems.

Techniques for identifying types of cells present in a biological sample using flow cytometry performed using a panel of markers and multiple machine learning models. The techniques include: obtaining flow cytometry data for the biological sample, the biological sample previously-obtained from a subject and comprising a plurality of cells, the flow cytometry data including flow cytometry measurements obtained during respective flow cytometry events, the flow cytometry events including a subset of events corresponding to cells in the biological sample being measured by the flow cytometry platform; and identifying types of cells of the plurality of cells using the multiple machine learning models to obtain a respective plurality of cell types.

The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.

A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. Systems and methods for determining data processing settings require an accurate measurement of peak times among various channels and being able to adjust time delay settings wherein peak time is the measurement of time elapsed from the beginning of the data collection time window to the highest peak in the window.

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.