A device and method of using the device to detect the presence and composition of clots and other target objects in a circulatory vessel of a living subject is described. In particular, devices and methods of detecting the presence and composition of clots and other target objects in a circulatory vessel of a living subject using in vivo photoacoustic flow cytometry techniques is described.

Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time. The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.

A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.

A cell observation system observes a cell moving in a flow path with a fluid, and includes a first imaging apparatus, a second imaging apparatus, and a control device. The first imaging apparatus includes a first optical system and a first imaging element, and captures an image of the cell at a first position in a moving direction. The second imaging apparatus includes a second optical system, in which a focus is adjusted based on a focus adjustment signal, and a second imaging element, and captures an image of the cell at a second position downstream of the first position. The control device obtains a passing position of the cell in a cross section of the flow path based on the image obtained by the first imaging element, generates the focus adjustment signal, and provides the signal to the second optical system.

A flow nanoparticle measurement device according to an embodiment of the present disclosure includes a flow cell in which a liquid sample flows, a first laser beam being irradiated to the flow cell; a laser generator configured to generate the first laser beam; and a flow controller configured to control a flow of the liquid sample for the flow cell.

The invention is based on a sensor device (10) with at least one sensor unit (12) comprising at least one laser unit (14) for a generation of at least one laser beam (16) and comprising at least one detection unit (18) for a detection of, in particular reflected, laser beams (20), with an evaluation unit (22) which is configured to process detected laser beams (20) into at least one sensor signal (39), and with a control unit (26) which is configured, in a continuous operation state, to actuate the sensor unit (12) and the evaluation unit (22) for an operation of the sensor unit (12) and the evaluation unit (22) in alternating switch-on intervals (31, 43) and switch-off intervals (33, 45). It is proposed that the evaluation unit (22) is configured to generate the at least one sensor signal (39) from at least two different switch-on intervals (31) of the sensor unit (12).

The present disclosure relates to systems and methods for quantifying mechanical properties of a cell containing a nucleus and cytoplasm. The system comprises a microfluidic comprises a varying width configured to deform the cell to multiple deformation levels, an imaging device configured to obtain image data of the cell received by the microfluidic channel and a processor in communication with the imaging device. The processor is configured to receive, from the imaging device, image data of the cell deformed within the microfluidic channel at a first deformation level and a second deformation level different from the first deformation level and to determine, based on the image data, one or more parameters associated with the deformed cell at the first deformation level and the second deformation level.

A flow cell according to an embodiment of the present disclosure includes a main flow portion configured to extend from one end in one direction and lead to other end, a pulsed laser beam being irradiated to the main flow portion, the main flow portion including a flow space in which a liquid sample flows; an inlet guide portion connected to the main flow portion in a different direction from the one direction and configured to guide an introduction of the liquid sample into the main flow portion; and an outlet guide portion connected to the main flow portion in a different direction from the one direction and configured to guide a discharge of the liquid sample from the main flow portion.

A sensing method of bioparticle positioning includes the steps of: providing a carrier divided into multiple detection areas; adding bioparticle sample in the carrier, wherein the bioparticle sample includes first bioparticle with biomarker and interacts with corresponding tag; providing excitation energy that makes the tag on the first bioparticle emit radioactive energy; moving the first sensor to the detection area respectively; after receiving radioactive energy, defining the detection area where the radioactive energy comes from as activity detection area, and sending location information of the activity detection area to processing module; according to location information, moving second sensor to detection area, detecting the accurate location of the first bioparticle in activity detection area, and sending the accurate location to processing module. A bioparticle positioning sensing system is also provided herein. The method and system above detect specific bioparticle quickly and improve the detection efficiency.

A particle inspection method includes irradiating a spatially modulated modulation beam onto a surface of a substrate and detecting an absorption light signal from a reflection beam generated through reflection of the spatially modulated modulation beam by the substrate.