Methods for characterizing spillover spreading originating from a first fluorochrome in fluorescent flow cytometer data collected for a second fluorochrome are provided. In some embodiments, methods include partitioning the fluorescent flow cytometer data according to the intensity of the data relative to the first fluorochrome. In embodiments, methods also include estimating with a first linear regression a zero-adjusted standard deviation for the intensity of light collected from the second fluorochrome for each of the partitioned quantiles based on the assumption that the intensity of light collected from the first fluorochrome is zero, and obtaining with a second linear regression a spillover spreading coefficient from the zero-adjusted standard deviations. Systems and computer-readable media for characterizing spillover spreading originating from a first fluorochrome in fluorescent flow cytometer data collected for a second fluorochrome are also provided.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

A method of representing a biological process via non-speech audio and system for carrying out same are provided. The method is effected by extracting a sequence of time-related biological events from the biological process and transforming the sequence of time-related biological events into rhythm and/or melody representative of the sequence of time-related biological events thereby representing the biological process via non-speech audio.

The present application discloses a nanoparticle recognition device and method based on detection of scattered light with electric dipole rotation. According to the scattering model of nanoparticles, the in situ detection of particle morphology in an optical trap is realized by the methods of particle suspension control and scattered light detection and separation. Specifically, two linearly polarized laser beams are used, wherein the first laser beam suspends nanoparticles and rotates nanoparticles by adjusting the polarization direction; the polarization direction of the second linearly polarized light is unchanged, and scattered light in a specific dipole direction is excited; the change of the polarizability of the nanoparticles is deduced by monitoring the change of the light intensity of the scattered light excited by the second laser beam at the fixed position, so that particle morphology recognition is realized.

An example apparatus comprises a first microfluidic channel fluidically coupled to a first reservoir containing a carrier fluid, the first microfluidic channel including a reaction region, a fluid droplet generator, and a fluid ejector fluidically coupled to the first microfluidic channel and disposed downstream from the reaction region of the first microfluidic channel. The fluid droplet generator includes a portion of the first microfluidic channel and a second microfluidic channel that intersects the first microfluidic channel and is fluidically coupled to a second reservoir containing a reaction fluid, where the reaction fluid including a plurality of cells and fluorescently-labeled capture reagents to form reaction products with a target molecule secreted by the plurality of cells.

A reagent selection support apparatus for supporting selection of a reagent used for cell measurement is provided. The apparatus includes a processing unit configured to acquire order information including a first measurement item and a second measurement item different from the first measurement item, and determine a combination of a first fluorescence reagent used to measure a first target molecule corresponding to the first measurement item and a second fluorescence reagent used to measure a second target molecule corresponding to the second measurement item, based on information on a property of the first target molecule and a property of a first fluorescent stain contained in the first fluorescent reagent, and information on a property the second target molecule and a property of a second fluorescent stain contained in the second fluorescent reagent; and an output unit configured to output the determined combination of the first fluorescence reagent and the second fluorescence reagent.

A MEMS-based particle manipulation system which uses a particle manipulation stage and a plurality of laser interrogation regions. The laser interrogation regions may be used to assess the effectiveness or accuracy of the particle manipulation stage. In one exemplary embodiment, the particle manipulation stage is a microfabricated, flap-type fluid valve, which sorts a target particle from non-target particles in a fluid stream. The laser interrogation stages are disposed in the microfabricated fluid channels at the input and output of the flap-type sorting valve. The laser interrogation regions may be used to assess the effectiveness or accuracy of the sorting, and to control or adjust sort parameters during the sorting process. One or more feedback loops may be used to improve the particle manipulation process, based on data acquired during the first interrogation and/or during a downstream confirmation. Artificial intelligence techniques may be used to good effect. A variable gain detector may improve the speed and sensitivity of the system.

A method comprises receiving images representing manipulating cellular bodies that includes exerting force pulses to the bodies on a wall surface; analyzing the images to determine the size of the bodies and tracking locations during and after each of force pulses, the tracking locations defining first trajectories of the bodies moving away from the wall surface and trajectories of the bodies moving towards the wall surface; determining densities of the bodies using the second trajectories and a sedimentation model of the bodies moving towards the wall surface and determining body velocities based on the first trajectories and a velocity model of the bodies moving away from the wall surface; and, determining a contrast factor for each body based on the sizes and the densities, the force applied to the bodies and the body velocities and determining a compressibility for each of the bodies based on the determined contrast factors.

Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles can have a refractive index substantially different from that of the channel and the medium, such that the particles interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be used for identifying, tracking, and sorting the particles.

Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.