Aspects of the present disclosure include circuits, systems and methods for baseline signal restoration over differential outputs. Circuits according to certain embodiments include an input module for receiving a signal from a sensor, an amplifier module, operably connected to the input module, for modifying the input signal, a baseline restoration module, operably connected to the amplifier module, for extracting a direct current component of the input signal, and an output module, operably connected to the amplifier module, for transmitting a baseline restored signal, wherein the output module comprises differential outputs. Baseline restoration systems according to certain embodiments include a baseline restoration circuit for generating a baseline restored signal on differential outputs, a downstream receiver circuit for receiving a baseline restored signal on differential inputs transmitted by the baseline restoration circuit, and cable core wires configured to connect the differential outputs of the baseline restoration circuit with the differential inputs of the downstream receiver circuit. Flow cytometry systems with baseline restoration using the subject circuits are described. Methods for baseline restoration are also provided.

Fluid management systems for flow type particle analyzers are provided. Aspects of the fluid management systems include a flow cell comprising an input and output; a cuvette comprising an input coupled to the output of the flow cell and further comprising an output; a sample input line for fluidically coupling a sample fluid source to the input of the flow cell; and a fluid supply subsystem configured to alternatively fluidically couple: (a) a primary fluid source; or (b) one or more secondary fluid sources, to the input of the flow cell. Also provided are methods of using flow type particle analyzers having the subject fluid management systems.

A system for orienting particles in a microfluidic system includes one or more radiation pressure sources arranged to expose particles to radiation pressure to cause the particles to adopt a particular orientation in the fluid. A system for sorting particles in a microfluidic system includes a detection stage arranged to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and one or more radiation pressure sources past which the particles move sequentially and a controller arranged to switch radiation energy to cause a change in direction of movement of selected particles in the fluid flow to sort the particles. The particles may be biological particles such as spermatozoa. The radiation pressure may be optical pressure and may be from one or more waveguides which may extend across a channel of the microfluidic system.

A sensor network, which includes a sensor controller serially coupled to a plurality of sensor modules, is configured to program the sensor modules so as to transfer measurement data to the sensor controller and to synchronize the sensor modules to picosecond accuracy via on-chip or on-module custom circuits and a physical layer protocol. The sensor network has applications for use in PET, LiDAR, FLIM and flow cytometry applications. Synchronization, within picosecond accuracy, is achieved through use of a picosecond time digitization circuit. The picosecond time digitization circuit is used to measure on-chip delays with high accuracy and precision. The delay measurements are directly comparable between separate chips even with voltage and temperature variations between chips.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

An object detection system compensates for variations in transmission characteristics within an object passageway caused by, for example, dust or dirt. An object detection device includes a plurality of electromagnetic radiation emitters and detectors arranged in rows on opposite sides of a passageway. First lenses focus radiation from the emitters into a semi-columnated beams of radiation that together create a plane of semi-columnated electromagnetic radiation that spans substantially across a width of the passageway. Second lenses focus the received electromagnetic radiation onto corresponding ones of the plurality of radiation detectors. A controller receives a radiation intensity signal from the detectors, determines that its value is outside of a predetermined range, and adjusts an amount of electrical power supplied to the plurality of radiation emitters so that the value of the radiation intensity signal changes to become within the predetermined range.

A device for analyzing cell morphology and a method for identifying cells are provided. A digital camera photographs a cell image of a blood sample under a low-magnification objective lens. A processor identifies and positions suspected cells of preset type in the cell image to obtain an identification result. Based on the identification result and a target number, the processor determines a number of suspected cells of preset type to be identified and positioned under the low-magnification objective lens. The digital camera further photographs, under a high-magnification objective lens, the suspected cells of preset type identified and positioned, and then the processor identifies whether the suspected cells of preset type photographed are cells of preset type, to count the number of cells of preset type photographed under the high-magnification objective lens and obtain a statistical value. If the statistical value≥the target number, photographing is stopped.

Provided are cell image analysis devices and sample analysis methods. A sample smear of a test sample is imaged by an imaging device in an assigned analysis mode to obtain first cell images of the test sample, which are identified and analyzed by a control device. If it is identified that there is preset abnormality in the sample smear, an analysis mode different from the assigned analysis mode and corresponding to the present abnormality is determined as an additional analysis mode, and the imaging device is controlled to image the sample smear in the additional analysis mode. The additional analysis mode matches with the preset abnormality, so that the imaging device is allowed to obtain cell images in the additional analysis mode, to identify and analyze the cell images matching the preset abnormality, thereby increasing processing efficiency and accuracy of processing result.

A high-throughput optofluidic device for detecting fluorescent droplets is disclosed. The device uses time-domain encoded optofluidics to detect a high rate of droplets passing through parallel microfluidic channels. A light source modulated with a minimally correlating maximum length sequences is used to illuminate the droplets as they pass through the microfluidic device. By correlating the resulting signal with the expected pattern, each pattern formed by passing droplets can be resolved to identify individual droplets.

Methods and systems for determining a drop delay of a flow stream in a flow cytometer are provided. Aspects of the methods according to certain embodiments include capturing an image of the flow stream to obtain an imaged flow stream, identifying a disturbance at a break off point in the imaged flow stream and calculating the drop delay of the flow stream based on the identified disturbance. Systems for practicing the subject methods having an imaging sensor for capturing one or more images of the flow stream and a processor configured to identify a disturbance at a break off in the imaged flow stream and calculating the drop delay using the imaged flow stream are also provided. Non-transitory computer readable storage mediums are also described.