An imaging system for imaging biological samples includes at least one main channel including at least one imaging space, the at least one main channel configured to transport the samples in a fluid, at least one reorientation unit configured to manipulate an orientation of the samples in the fluid, and at least one imaging unit configured to receive detection light emitted by the samples in the at least one imaging space.

The systems and methods provided herein relate generally to the improvement of data quality in optical liquid particle counters and control of optical particle counters to achieve longer expected lifetime, for example by avoiding damage caused by electromagnetic radiation and heat. The systems and methods incorporate sensors which characterize the fluid flowing through the flow cell, thereby enhancing accuracy and reducing the number of false positives.

Various embodiments provide a particle sensor including: a first carrier, the first carrier including at least one heating structure and a light detecting structure, at least one spacer structure disposed over the first carrier, a second carrier disposed over the at least one spacer structure, the second carrier including a light emitting structure, wherein the first carrier, the second carrier and the at least one spacer structure are arranged to provide a channel for a fluid flow, wherein the light emitting structure is configured to emit light into the channel and wherein the light detecting structure is configured to detect light from the channel.

A method of imaging a sample by interferometric scattering microscopy, the method comprising illuminating a sample with at least one light source, the sample being held at a sample location comprising a reflective surface, such that a reflected signal is formed; the reflected signal comprising light from the light source and light scattered by the sample; detecting the output light over a first time window for a first frame N.sub.1; detecting the output light over a second time window for a second frame N.sub.2; calculating a ratiometric signal R which is the ratio of N.sub.1 and N.sub.2 minus 1; estimating the ratiometric motion signature S=(S.sub.x, S.sub.y) from frames N.sub.1 and N.sub.2 with S defined as the ratiometric image which would be measured from an invariant sample moving along x and y for a given motion vector m=(m.sub.x, m.sub.y); estimating m as the most consistent vector such that R is approximated using S and m; calculating the corrected ratiometric contrast image R* from R, S and m.

Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

Method of and apparatus for performing cyclic flow cytometry analysis on a sample population of cellular entities including: causing each cellular entity to be labeled with an optical identifier; for each cellular entity, performing a first pass of flow cytometry measurement over a flow channel with respect to a first set of parameters, under conditions of determining an identification for the cellular entity for which values of the first set of parameters are being obtained, and storing the values of the first set in association with the identification; and performing a second pass of flow cytometry measurement over the flow channel with respect to a second set of parameters, under conditions of separately determining an identification for the cellular entity for which values of the second set of parameters are being obtained, and storing the values of the second set in association with the identification.

Methods for characterizing spillover spreading originating from a first fluorochrome in fluorescent flow cytometer data collected for a second fluorochrome are provided. In some embodiments, methods include partitioning the fluorescent flow cytometer data according to the intensity of the data relative to the first fluorochrome. In embodiments, methods also include estimating with a first linear regression a zero-adjusted standard deviation for the intensity of light collected from the second fluorochrome for each of the partitioned quantiles based on the assumption that the intensity of light collected from the first fluorochrome is zero, and obtaining with a second linear regression a spillover spreading coefficient from the zero-adjusted standard deviations. Systems and computer-readable media for characterizing spillover spreading originating from a first fluorochrome in fluorescent flow cytometer data collected for a second fluorochrome are also provided.

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

Systems having an unfiltered light scatter detector configured to detect scattered light from a sample in a flow stream are provided. Systems according to certain embodiments include a light source having two or more lasers, a light detection system having an unfiltered light scatter detector and a processor having memory operably coupled to the processor where the memory includes instructions which when executed by the processor, cause the processor to generate one or more data signals in response to scattered light from each of the two or more lasers detected by the unfiltered light scatter detector; and determine one or more parameters of data acquisition based on the generated data signals from the unfiltered light scatter detector. Methods for determining one or more parameters for data acquisition with the subject systems are also described.