Aspects of the present disclosure include methods for determining a photodetector gain correction factor for application to flow cytometer data. Methods according to certain embodiments include detecting light with a light detection system across a horizontal axis of a flow stream, generating data signals in a photodetector channel (e.g., an imaging photodetector channel) of the light detection system at a plurality of positions across the flow stream and calculating a detector gain correction factor for each position across the flow stream in response to the generated data signals. Methods also include applying a detector gain correction factor to data signals from a photodetector channel (e.g., non-imaging photodetector channels) to generate adjusted signal intensities. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector (e.g., an imaging photodetector) for practicing the subject methods are also described. Non-transitory computer readable storage medium and integrated circuits (e.g., FPGAs) are also provided.

A fine particle measuring device performing fine particle measurement includes a particle probe, a pipe connected to the particle probe, and a particle counter connected to the pipe. A cylindrical pipe is disposed on an outer periphery of the pipe and an air flow path is provided between the pipe and the cylindrical pipe.

A system comprises a particle sensor unit in communication with a processor. The sensor unit comprises a source that transmits light into an interrogation region; receive optics that collect scattered light from particles in the interrogation region; and an optical detector that receives the collected light from the receive optics. The detector comprises a sample area including one or more sampling pixels, and an edge region including one or more edge pixels. The processor analyzes intensity data from the detector by a method comprising: combining all intensity data from the sampling pixels; adding the combined intensity data to a data set; determining whether to accept overlap intensity data that corresponds to an overlap between the sampling pixels and the edge pixels; adding the overlap intensity data to the data set if accepted; discarding the overlap intensity data if not accepted; and discarding all non-overlapping intensity data from the edge pixels.

The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.

Condensation associated with the collection and identification of airborne particles is detected. Upon the detection, one or more condensation countermeasures are triggered to address the condensation.

Provided is a high efficiency and high sensitivity particle capture type terahertz sensing system. The particle capture type terahertz sensing system includes a sensing substrate to capture particles, and a terahertz sensor to emit terahertz electromagnetic waves to the sensing substrate to sense the particles, wherein the sensing substrate includes a base substrate and a particle capture structure layer formed on the base substrate, the particle capture structure layer includes a plurality of slits for focusing the terahertz electromagnetic waves, the particle capture structure layer captures the particles in the plurality of slits using dielectrophoresis, and an area in which the terahertz electromagnetic waves converge to the plurality of slits matches an area in which the particles are captured in the plurality of slits through the dielectrophoresis.

A particle sizing method which allows for counting and sizing of particles within a colloidal suspension flowing through a single-particle optical sizing sensor SPOS apparatus using pulse height detection and utilizing non-parallel and non-uniform illumination within the sensing region of the flow cell. The method involves utilizing a deconvolution process which requires the SPOS apparatus to be characterized during a calibration phase. Once the SPOS apparatus has been characterized, the process of deconvolution after a data collection run, recursively eliminates the expected statistical contribution to the pulse height distribution PHD histogram in all the lower channels from the highest channel height detected, and repeating this for all remaining channels in the PHD, removing the contributions from largest to smallest sizes.

The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.