To provide a microparticle measurement technology capable of supporting excitation light in a wideband wavelength range.

The present technology provides a microparticle measurement device provided with a plurality of objective lenses for excitation light irradiation used for irradiating microparticles flowing through a flow path with excitation light, in which at least one of the objective lenses for excitation light irradiation is used for detecting scattered light emitted from the microparticles by the excitation light with which the microparticles are irradiated through another one of the objective lenses for excitation light irradiation.

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

In one example in accordance with the present disclosure, a system is described. The system includes a fluidic die to advance across an ejection path relative to a substrate. The fluidic die includes a channel to contain a portion of a sample fluid, a sensor to detect passage of a particle within the sample fluid into the channel, and an ejection device. The ejection device is to eject the particle. The system also includes a controller. The controller identifies discrete locations along the ejection path as waste sites as the fluidic die advances along the ejection path. This is done by 1) classifying the particle as a target particle or a non-target particle, 2) upon identification of a target particle, ejecting the target particle to a target site of the substrate, and 3) upon identification of a non-target particle, ejecting the non-target particle to a waste site.

A disposable injection moulded flow cell or cuvette for use in flow cytometer for in vitro assaying of human or animal whole blood and to an investigation method using the flow cytometer. The present disclosure provides a cuvette for use in an optical flow cytometer, comprising a cuboid sheath preparation area, a curved sample injection area with a rectangular cross section, a pyramidal shaped flow formation area, and a sample injector which is arranged in the transition area from the cuboid sheath preparation area to the curved sample injection area.

The present disclosure provides an integrated collection, filtration, and analysis system which is configured to automatically collect fluid samples from a surrounding open water environment, isolate floating particles of a target size range, and perform analysis on the collected particles in-situ. The particles may also be filtered by one or more parameters other than size, and also provided herein is a two-stage vortex filter specially adapted to isolate floating particles of a desired density range from a fluid.

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

Provided are an improved holographic reconstruction apparatus and method. A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; extracting reference light information from the obtained object hologram; calculating a wavenumber vector constant of the extracted reference light information, and generating digital reference light by calculating a compensation term of the reference light information by using the calculated wavenumber vector constant; extracting curvature aberration information from the object hologram, and then generating digital curvature in which a curvature aberration is compensated for; calculating a compensated object hologram by multiplying the compensation term of the reference light information by the obtained object hologram; extracting phase information of the compensated object hologram; and reconstructing 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object by calculating the quantitative thickness information of the measurement target object by using the extracted phase information of the compensated object hologram.

A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.

A complex particle measurement apparatus comprising a first light source that irradiates a first storage cell; a photodetector that detects intensity of light; a second light source that irradiates a second storage cell; an imaging unit that images a particle group; an image data output unit that outputs image data; a supporter that supports the first storage cell and the second storage cell; and a communication pipe that connects the first storage cell and the second storage cell to pass a sample solution, wherein the first storage cell and the second storage cell have bottom surfaces located at positions different from each other, and the communication pipe is laid such that a channel from the first storage cell to the second storage cell has an incline of not less than 0 or not more than 0.