To provide an adjusting method of a positional relationship between a flow path position and a light irradiation position.

The present technology provides a position adjusting method provided with an imaging step of imaging, while moving a flow path through which a microparticle is able to flow in an optical axis direction, the flow path in a plurality of positions in the optical axis direction, a movement step of moving the flow path in the optical axis direction on the basis of a focus index for each of a plurality of images acquired at the imaging step, and an adjustment step of specifying a feature position of the flow path from an image of the flow path in a position after movement at the movement step, and adjusting a positional relationship between the feature position and a reference position in a direction perpendicular to the optical axis direction.

This flow cytometer includes a flow path through which an observation object flows with a fluid; an optical illumination system including a spatial optical modulation device, and a first optical element; and an optical detection system including a first light detector, wherein the optical illumination system further includes a first spatial filter disposed in a first optical path between a light source and an image position of light imaged in the flow path by the first optical element and having a first region which hinders traveling of light emitted from the light source towards the observation object, the optical detection system further includes a second light detector disposed in a second optical path between the first light detector and the image position and having a second region which directs the light modulated by the observation object towards the first light detector, and the position of the first region and the position of the second region are in a substantially optically conjugate relationship.

A powder flow monitoring system may include a computing device configured to receive image data representing illuminated powder of a powder stream between a powder delivery device and a build surface of a component, generate a representation of the powder stream based on the image data, and output the representation of the powder stream for display at a display device.

The invention relates to a method for producing at least one pattern (2) on a carrier surface (7) of a carrier (3), wherein the method comprises the following steps: a. adding a first fluid (4) to the carrier surface (7) and b. adding a second fluid (5), wherein the second fluid (5) is immiscible with the first fluid (4) and at least partially covers the first fluid (4) and c. adding at least one object (6) above the carrier surface (7), d. generating the pattern (2) by a relative movement between the object (6) and the carrier (3) in which a force is exerted on the object (6) from a force generating means (28), wherein the force transmission from the force generating means (28) on the object (6) is contactless and the pattern (2) is generated by a portion of the second fluid (15) wetting the carrier surface (7).

The method can include: acquiring an image of an arrangement having at least one cell covering at least one adhesive surface area, the at least one adhesive surface area being predictively deformable upon exerting cellular forces stemming from said at least one cell; measuring, from the image, a surface area of the at least one cell; comparing the measured surface area to a reference surface area value; and generating a signal indicative of the cellular forces based on said comparison.

An information processing apparatus that executes processing of obtaining, from an interference fringe image that is a two-dimensional distribution of intensity of interference fringes of object light and reference light, a phase difference image that is a two-dimensional distribution of a phase difference between the object light and the reference light, and obtaining a shape of an object to be observed based on the phase difference image includes at least one processor configured to acquire object-related information regarding the object to be observed, read out, from a storage unit in which the object-related information and a shape profile indicating the shape of the object to be observed are stored in association with each other, the shape profile corresponding to the acquired object-related information, and perform phase connection with respect to the phase difference image with reference to the read-out shape profile.

Among other things, the present invention is related to devices and methods of performing biological and chemical assays, such as but not limited to assay related to analysis of white blood cells.

Embodiments of the invention are directed to an optoelectronic device for detection and identification of individual water droplets, ice crystals, dust particles and volcanic ash particles, the device comprising a source of ultraviolet collimated monochromatic radiation that illuminates an area of air external to the aircraft through which freely pass individual atmospheric particles to create an illuminated sample volume of air; an optical surveillance system for monitoring the clarity of light transmission through the light transmissive window to indicate a need for preventive maintenance; a first optical detection system that is constructed and arranged to collect light scattered from individual particles over an explicit angle ranging from 137° to 173° that defines the illuminated sample volume for measurement of S and P components of return scattered light from the sample volume to photodetectors that provide signals representative of intensity and change in polarization state caused by the interaction of particles with the incident illumination in the sample volume; a second optical detection system for selectively detecting fluorescence emanating from individual ash particles over an explicit angle ranging from 137° to 173° that defines the illuminated sample volume for measurement of fluoresence from the sample volume to a photodetector that provide a signal representative of intensity caused by the interaction of particles with the incident illumination in the sample volume; a signal processor that is constructed and arranged to condition the signals from the photodetectors by removing electronic noise, restoring baseline shifts and analyzing the pulse shapes to provide processed signals; a signal analyzer configured to operate upon the processed signals for extraction of data representing maximum amplitude, width, rise time and fall time of individual pulses in the S and P components, and the magnitude of the fluorescence signal which correlates to the size and/or composition of ash particles present; an information synthesizer that receives the data and produces analytical results allocated to particles by particle type including equivalent optical diameter (EOD), number and mass size distributions, and number and mass concentrations, the particle type being selected as at least one member among the group consisting of individual water droplets, ice crystals, dust particles and volcanic ash particles; and a report generator that creates an information packet utilizing information from the information synthesizer to assist in decision making related to hazard avoidance for aircraft flight, the optoelectronic system being adapted for mounting

An optical method of characterizing an object comprises providing an object to be characterized, the object having at least one nanoscale feature; illuminating the object with coherent plane wave optical radiation having a wavelength larger than the nanoscale feature; capturing a diffraction intensity pattern of the radiation which is scattered by the object; supplying the diffraction intensity pattern to a neural network trained with a training set of diffraction intensity patterns corresponding to other objects with a same nanoscale feature as the object to be characterized, the neural network configured to recover information about the object from the diffraction intensity pattern; and making a characterization of the object based on the recovered information.

A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.