Light detection devices and corresponding methods are provided. The devices include a reaction structure to contain a reaction solution and at least one reaction site that generates light emissions in response to incident excitation light after treatment with the reaction solution. The devices also include a plurality of light sensors and device circuitry. The devices further include a plurality of light guides extending toward at least one corresponding light sensor from input regions that receive the excitation light and the light emissions from at least one corresponding reaction recess. The light guides comprise a first filter region that filters the excitation light and permits the light emissions of a first wavelength to pass to the at least one corresponding light sensor, and a second filter region that filters the excitation light and the permits light emissions of a second wavelength to pass to the at least one corresponding light sensor.

Analyte collection and testing systems and methods, and more particularly to testing systems and methods that achieve significant improvements in the detection of fluorescence signals in the reader by modulating the applied optical excitation. Also described herein are optical detection apparatuses and methods for removable photonic chips that do not require translation for calibration when coupling the photonics chip with the sensing system. Also described herein are methods and apparatuses for accurately calibrating a dilution factor when reading from a photonics chip.

A bio-related substance assay tube 2 comprises a target substance capture bead 3 serving as a first microparticle, compensation bead 4 serving as a second microparticle on which a given amount of a bio-related substance has been immobilized, and a negative control bead 5 serving as a third microparticle for use as a negative control. A mount unit 12 comprises a nozzle communicating with a pump, and the bio-related substance assay tube 2 is mounted in the mount unit 12 to ensure communication with this nozzle. An analyte is introduced into the bio-related substance assay tube 2, followed by labeling the bio-related substance bound to each microparticle to cause light emission. Based on light emission from each microparticle, a calibration curve is prepared or the emission intensity of the first microparticle is compensated to quantify the bio-related substance.

This disclosure provides a diagnostic system including a detection zone adapted to receive a volume of biological fluid. The detection zone includes a plurality of micro-scale and nano-scale features that render the detection zone superhydrophobic. Analytes (e.g., proteins and/or other molecules) are concentrated when the volume of biological fluid is allowed to evaporate on the detection zone. Concentrating the analytes in the detection zone by evaporation can advantageously increase the sensitivity of detection of the analyte. In various implementations, microfluidic channels can be integrated with the diagnostic system to convey the volume of biological fluid to the detection zone. In various implementations, the microfluidic channels can have a lower hydrophobic characteristic than the surrounding to realize self-driven microfluidic channels that convey the biological fluid to the detection zone without using any external devices.

Devices and methods for calculating various coagulation characteristics associated with a patients liquid test sample. The presently disclosed and claimed inventive concept(s) relate to an improved device(s) and method(s) for conducting coagulation assays on a patients liquid test sample, including, without limitation, a patients whole blood sample.

Among other things, certain embodiments of the present disclosure are related to devices and methods of performing biological and chemical assays, such as but not limited to pH measurement of bio/chemical samples.

The invention relates to the field of integrated systems which comprise a test element and a lancet which can be firstly used to make a wound in a skin opening in order to collect a sample. The sample is subsequently directly taken up by the test element in the process of which it comes into contact with a reagent contained in the test element and results in an optically detectable change in a test field. The change in the test field is detected by means of an analytical unit which is optically contacted with the test field via at least one light-conducting element.

The present disclosure relates to a biochemical detection instrument. The technical solution is an automatic reactive oxygen species content detection system suitable for a cell microenvironment that includes: a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline. A washing system is in communication with the sample transmission reaction system through a water pipeline, and a purge system is in communication with the sample transmission reaction system through a gas pipeline. The sample transmission reaction system further includes a sample injector and a DCFH supply bin which are communicated with a reaction bin through light avoiding pipelines after being connected in parallel. Sample injection valves are respectively configured between the sample injection valve and the reaction bin and between the DCFH supply bin and the reaction bin.

A system and process may be used to test water samples to measure ATP and/or estimate a microbial population, for example using an adenosine triphosphate (ATP) based assay. The system includes a device that is use in combination with single-use or disposable cartridges. The cartridge receives the water sample and is pre-loaded with one or more reagents. The device receives the cartridge and contains physical, electronic and/or mechatronic devices that interact with cartridge. One or more actions such as metering, mixing and conveying are performed automatically by elements of the device and/or cartridge. A sensor in the device measures light produced in the cartridge from a reaction with ATP in the water sample. Optionally, the cartridge also contains a pre-loaded amount of ATP, which is used to provide an internal reference or calibration measurement.

Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.