Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor. A flow cytometer may include a flow cell, a laser, a first optical sensor positioned to measure scattered laser light, a second optical sensor positioned to measure fluorescent emissions, and a controller configured to adjust the measurements taken by the second optical sensor based on a comparison of measurements taken by the first optical sensor with expected measurements based on a known beam profile of the laser beam.

Provided herein are analyzers as well as related methods for measuring both an absorbance and emission of a sample. The analyzer includes light sources for epi-illumination and transillumination of the sample, and detectors for measuring the intensities of excitation, emission, and transillumination light. A dichroic mirror permits a portion of the excitation light to transmit to a detector that monitors changes in excitation light intensity. Temperature sensors allow for signal corrections based on temperature variations of the detectors and sample.

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WBCs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90 polarized side scatter, (4) 90 depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor. A flow cytometer may include a flow cell, a laser, a first optical sensor positioned to measure scattered laser light, a second optical sensor positioned to measure fluorescent emissions, and a controller configured to adjust the measurements taken by the second optical sensor based on a comparison of measurements taken by the first optical sensor with expected measurements based on a known beam profile of the laser beam.

The present disclosure relates to a fluorescence filter for measuring fluorescence generated by a measurement object and an image sensor module including the same, and includes an absorption filter transmitting light within a specific wavelength band generated by the measurement object including a fluorescent dye and absorbs light in the remaining wavelength bands, and a reflection filter that is disposed adjacent to the absorption filter, transmits light within a specific wavelength band generated by the measurement object, and reflects light in the remaining wavelength bands, wherein the absorption filter has a plurality of wells having a predetermined depth in which the measurement object is accommodated, and wherein the plurality of wells are disposed at regular intervals on an incident surface of the absorption filter to which external light is incident.

The present invention is drawn to devices and systems for allergen detection in a sample. The allergen detection system includes a sampler, a disposable analysis cartridge and a detection device with an optimized optical system. In some embodiments, the allergen detection utilizes aptamer nucleic acid molecules as detection agents. In some embodiments, the nucleic acids are conjugated to magnetic beads or solid surfaces such as glasses, microwells and microchips.

A system and method for determining impurities in a beverage grade gas such as CO.sub.2 or N.sub.2 relies on a coupling of FTIR analysis and UV fluorescence detection. Conversion of reduced sulphur present in some impurities to SO.sub.2 can be conducted using a furnace. In some cases, CO.sub.2% also is determined.

A multi-mode illumination system, including: a first illumination module; a second illumination module; and a third illumination module, as disclosed herein.

Techniques are for detecting presence of a problematic cellular entity in a target. In an example, using an analysis model, a fluorescence-based image is analyzed. The analysis model is trained using a number of reference fluorescence-based images for detecting the presence of problematic cellular entities in targets. Based on the analysis, a problematic cellular entity present in the target is detected. To perform the detection, the analysis model is trained to differentiate between the fluorescence in the fluorescence-based image emerging from the problematic cellular entity and the fluorescence in the fluorescence-based image emerging from regions other than the problematic cellular entity.

Systems and methods for determining properties of gemstones based, inter alia, on fluorescence properties of the gemstones, are presented. In one aspect, properties of at least one gemstone can be determined. In another aspect, a relationship between at least two gemstones can be determined. In one example, a first and a second gemstones are illuminated with illuminating light of at least one fluorescence-exciting wavelength range; corresponding at least one first fluorescence-emission light and at least one second fluorescence-emission light spectrum, emitted from the first and second gemstones respectively are detected and analyzed, either independently or by comparison, to determine the relationship between the first and second gemstones. In some examples, data indicative of visible light absorbance or three-dimensional models of the gemstones is combined with the fluorescence data to determine the properties or the relationship.