The present invention is directed to a method for the determination of the hyaluronic acid content of a hydrogel, the method comprising the following steps: a) preparing, as reagent A, a solution of sodium tetraborate in sulfuric acid; b) preparing reagent B by dissolving carbazole in ethanol; c) preparing test solutions by dissolving the hydrogel in an aqueous solution; d) treating the test solution with ultrasounds for a period of time sufficient to obtain a macroscopically homogeneous solution; e) preparing a reference stock solution by dissolving glucuronic acid, or a glucuronic acid-containing substance in an aqueous solution; f) preparing at least 3 reference solutions by dilution of the reference stock solution in aqueous solution, preferably at concentration comprised between 0.0005% w/v and 0.0100% w/v, preferably between 0.0010% w/v and 0.0050% w/v; g) preparing the test tubes by admixing reagent A, reagent B and one of the following: reference solution, test solution, aqueous solution (blank), and optionally solution for interference (crosslinker sample or additive sample); placing each test tube on a water bath for at least 5 min, then cool them to room temperature; h) reading the absorbance at a wavelength comprised between 500 and 580 nm, preferably at about 530 nm, against the blank and optionally the sample for interference.

The present invention is directed to a method for the determination of the hyaluronic acid content of a hydrogel, the method comprising the following steps: a) preparing, as reagent A, a solution of sodium tetraborate in sulfuric acid; b) preparing reagent B by dissolving carbazole in ethanol; c) preparing test solutions by dissolving the hydrogel in an aqueous solution; d) treating the test solution with ultrasounds for a period of time sufficient to obtain a macroscopically homogeneous solution; e) preparing a reference stock solution by dissolving glucuronic acid, or a glucuronic acid-containing substance in an aqueous solution; f) preparing at least 3 reference solutions by dilution of the reference stock solution in aqueous solution, preferably at concentration comprised between 0.0005% w/v and 0.0100% w/v, preferably between 0.0010% w/v and 0.0050% w/v; g) preparing the test tubes by admixing reagent A, reagent B and one of the following: reference solution, test solution, aqueous solution (blank), and optionally solution for interference (crosslinker sample or additive sample); placing each test tube on a water bath for at least 5 min, then cool them to room temperature; h) reading the absorbance at a wavelength comprised between 500 and 580 nm, preferably at about 530 nm, against the blank and optionally the sample for interference.

A detection device for detecting the presence of a substance of interest in a sample is described. The device can include a data store comprising executable instructions for at least one convolutional neural network, CNN, configured to process images: and a processor coupled to the data store and configured to execute the instructions to operate the at least one CNN. The detection device can be configured to: obtain spectrometry data, operate a first one of the CNNs to process the spectrometry data to obtain a first CNN output; apply a mask to the spectrometry data to obtain masked data; operate a second one of the CNNs to process the masked data to obtain a second CNN output; and determine if the substance of interest is present in the sample based on both the first CNN output and the second CNN output.

A seamless fumed silica monolithic integrating cavity device tailored to analyzing a flowed sample. The device is configured to facilitate optical measurements taken from a sample flowed through a cavity of the device. The cavity is defined by a fumed silica monolith with the added feature of a fused quartz lining on the surface of the monolith. This provides an intermediate surface that allows for cleaning and reuse of the highly effective diffuse light scattering fumed silica monolith. The lining may be placed under pressure or vacuum to structurally enhance mechanical integrity of the underlying monolith. Thus, continued or reliably repeated use of the device may be appreciated as well as use in more industrial environments that are prone to vibration. Additionally, while well suited for flow-based sample analysis, a valve of the cavity may be utilized for holding a sample in a temporarily static state for measurement.

A seamless fumed silica monolithic integrating cavity device tailored to analyzing a flowed sample. The device is configured to facilitate optical measurements taken from a sample flowed through a cavity of the device. The cavity is defined by a fumed silica monolith with the added feature of a fused quartz lining on the surface of the monolith. This provides an intermediate surface that allows for cleaning and reuse of the highly effective diffuse light scattering fumed silica monolith. The lining may be placed under pressure or vacuum to structurally enhance mechanical integrity of the underlying monolith. Thus, continued or reliably repeated use of the device may be appreciated as well as use in more industrial environments that are prone to vibration. Additionally, while well suited for flow-based sample analysis, a valve of the cavity may be utilized for holding a sample in a temporarily static state for measurement.

A system for generating hyperspectral imaging data for measuring biochemical compositions is disclosed which includes a spectral imaging device adapted to acquire one or more hyperspectral linescan images, an optical imaging device with a red-green-blue (RGB) sensor adapted to acquire an RGB dataset, a processor adapted to co-locate a plurality of pixels in the RGB dataset vs. a corresponding plurality of pixels of the one or more hyperspectral linescan datasets, establish a transformation matrix utilizing the plurality of co-located pixels, apply the transformation matrix to the RGB dataset to thereby generate the hyperspectral dataset, and analyze the generated hyperspectral image dataset to determine the biochemical compositions.

A system for generating hyperspectral imaging data for measuring biochemical compositions is disclosed which includes a spectral imaging device adapted to acquire one or more hyperspectral linescan images, an optical imaging device with a red-green-blue (RGB) sensor adapted to acquire an RGB dataset, a processor adapted to co-locate a plurality of pixels in the RGB dataset vs. a corresponding plurality of pixels of the one or more hyperspectral linescan datasets, establish a transformation matrix utilizing the plurality of co-located pixels, apply the transformation matrix to the RGB dataset to thereby generate the hyperspectral dataset, and analyze the generated hyperspectral image dataset to determine the biochemical compositions.

The single-use disposable spectrophotometer described herein can measure one or more blood chemistry analytes from a drop of whole blood. A passive filtration system takes whole blood and delivers plasma along with a dissolved reporter molecule to one or more spectrophotometers which can operate with narrow band optical spectrum centered on an optical detection frequency. The spectrophotometer detects the changes in absorption of the plasma as a result of a chemistry reaction to determine the concentration or activity of one or more analytes.

The single-use disposable spectrophotometer described herein can measure one or more blood chemistry analytes from a drop of whole blood. A passive filtration system takes whole blood and delivers plasma along with a dissolved reporter molecule to one or more spectrophotometers which can operate with narrow band optical spectrum centered on an optical detection frequency. The spectrophotometer detects the changes in absorption of the plasma as a result of a chemistry reaction to determine the concentration or activity of one or more analytes.

The present technology relates to an imaging apparatus, an imaging method, and a program that perform appropriate exposure control, to thereby enable a desired object to be appropriately imaged. The present technology includes: an imaging unit including a plurality of pixels having different spectral characteristics; and an exposure control unit setting information associated with exposure control on the plurality of pixels depending on specification information for specifying a kind of a measurement target. Alternatively, the present technology includes: an imaging unit including a plurality of pixels having different spectral characteristics; and an exposure control unit setting information associated with exposure control on the plurality of pixels on the basis of a predicted output value of each of the plurality of pixels based on a spectral characteristic related to a measurement target. The present technology is applicable to an imaging apparatus which senses vegetation, for example.