The present invention is directed to a method and an apparatus for detecting micro-colonies growing on a membrane or an agarose medium of a sample in a closed device. According to the invention the sample is irradiated with a light incident at an angle () with respect to the normal to the membrane or the surface of the agarose medium from outside the device. An incident area of the light on the membrane or the surface of the agarose medium is imaged by means of a light receiving element using an imaging angle () different from angle () with respect to the normal to the membrane or the surface of the agarose medium from outside the device. The light reflected, scattered and/or diffused from the membrane or the surface of the agarose medium and/or the micro-colonies on the membrane and/or the micro-colonies on the agarose medium is detected.

Microfluidic methods and systems for detecting levels of microorganisms via Mie forward light scattering. The systems and methods of the present invention can be customized so as to optionally maximize scatter from the particle immunoagglutination and minimize that of the sample matrix, e.g., via selection of parameters particle diameter d, wavelength of incident , and/or scatter angle . Methods feature providing beads conjugated with an antibody specific for the microorganism, mixing beads with a sample to form a first mixture; providing a control mixture; irradiating the mixtures with incident light; detecting forward light scattering scattered by the first mixture at a first angle with respect to the incident light and forward scattered light scattered by the control the same first angle with respect to the incident light; determining I and I.sub.0 from the light scattering; comparing I with I.sub.0; and determining a level of the microorganism.

Devices and methods for using changes in the defects in micrometer sized dispersed liquid crystal domains to detect or quantify analytes in a test sample, including endotoxin lipopolysaccharide (LPS), are disclosed. The dispersed liquid crystal microdomains are exposed to the test sample, and any changes in the number of defects in the liquid crystal microdomains are detected by detecting changes in the anchoring configuration of the microdomains. Such changes in anchoring configuration indicate the presence of analyte in the test sample.

A system is provided. The system includes a light source configured to emit a probing beam to illuminate an optical element. The system also includes an image sensor configured to be rotatable around the optical element within a predetermined rotation range. The system also includes a controller configured to control the image senor to move to a plurality of angular sub-ranges of the predetermined rotation range to receive a plurality of scattered beams output from the optical element. The image sensor is configured to generate a plurality of sets of speckle pattern image data based on the received scattered beams. The sets of speckle pattern image data provide two-dimensional (2D) spatial information of speckles.

Methods for determining an assumption-free radius of gyration of particles in solution using a light scattering detector are disclosed. The method may include determining a first weighting factor based on a first form factor. The method may also include determining a second weighting factor based on a second form factor. The method may further include determining a first form factor contribution for the first form factor based on the first weighting factor and the second weighting factor. The method may also include determining a second form factor contribution for the second form factor based on the first weighting factor and the second weighting factor. The method may also include determining the assumption-free radius of gyration from the first and second form factor contributions.

An apparatus for sensing particulate matter in a fluid includes a fluid flow conduit fluidically connected to an interaction chamber; a light source positioned to illuminate the interaction chamber; and a light detector assembly positioned to receive light scattered by particulate matter present in the interaction chamber. The light detector assembly includes a light detector; and an optical element, the optical element configured to provide light to the light detector based on an incidence angle of the scattered light.

According to one embodiment, an optical test apparatus includes a first aperture, a second aperture, an image sensor, and a first lens. The first aperture includes a first aperture plane provided with a first wavelength selecting region. The second aperture includes a second aperture plane provided with a second wavelength selecting region different from the first wavelength selecting region. The image sensor is configured to image a light beam passing through the first aperture plane and the second aperture plane and reaching an imaging plane. The first lens is configured to make a light beam passing through the first aperture plane and the second aperture plane be incident on the imaging plane.

An egg analysis system and computer-implemented methods are provided. The system comprises a light source for illuminating an egg, a photon detector for detecting a fluctuation of scattering light emitted from the egg, a processor, and a memory storing instructions which when executed by the processor configure the processor to receive scattering light data from the photon detector, digitize the scattering light data, and analyze the digitized scattering light data. One computer-implemented method comprises a light source illuminating an egg, a photon detector detecting a fluctuation of scattering light emitted from the egg, receiving scattering light data from the photon detector, digitizing the scattering light data, and analyzing the scattering light data. Another computer-implemented method comprises receiving angle, frequency and intensity data of scattering light from an egg illuminated using a light source, identifying a germinal disc in the egg from the scattering light data, and determining at least one of a fertility or a sex of the egg based on the size and structure of the germinal disc.

An egg analysis system and computer-implemented methods are provided. The system comprises a light source for illuminating an egg, a photon detector for detecting a fluctuation of scattering light emitted from the egg, a processor, and a memory storing instructions which when executed by the processor configure the processor to receive scattering light data from the photon detector, digitize the scattering light data, and analyze the digitized scattering light data. One computer-implemented method comprises a light source illuminating an egg, a photon detector detecting a fluctuation of scattering light emitted from the egg, receiving scattering light data from the photon detector, digitizing the scattering light data, and analyzing the scattering light data. Another computer-implemented method comprises receiving angle, frequency and intensity data of scattering light from an egg illuminated using a light source, identifying a germinal disc in the egg from the scattering light data, and determining at least one of a fertility or a sex of the egg based on the size and structure of the germinal disc.

According to one embodiment, an optical test apparatus includes a first aperture, a second aperture, an image sensor, and a first lens. The first aperture includes a first aperture plane provided with a first wavelength selecting region. The second aperture includes a second aperture plane provided with a second wavelength selecting region different from the first wavelength selecting region. The image sensor is configured to image a light beam passing through the first aperture plane and the second aperture plane and reaching an imaging plane. The first lens is configured to make a light beam passing through the first aperture plane and the second aperture plane be incident on the imaging plane.