This relates to systems and methods for measuring a concentration and type of substance in a sample at a sampling interface. The systems can include a light source, optics, one or more modulators, a reference, a detector, and a controller. The systems and methods disclosed can be capable of accounting for drift originating from the light source, one or more optics, and the detector by sharing one or more components between different measurement light paths. Additionally, the systems can be capable of differentiating between different types of drift and eliminating erroneous measurements due to stray light with the placement of one or more modulators between the light source and the sample or reference. Furthermore, the systems can be capable of detecting the substance along various locations and depths within the sample by mapping a detector pixel and a microoptics to the location and depth in the sample.

This relates to systems and methods for measuring a concentration and type of substance in a sample at a sampling interface. The systems can include a light source, optics, one or more modulators, a reference, a detector, and a controller. The systems and methods disclosed can be capable of accounting for drift originating from the light source, one or more optics, and the detector by sharing one or more components between different measurement light paths. Additionally, the systems can be capable of differentiating between different types of drift and eliminating erroneous measurements due to stray light with the placement of one or more modulators between the light source and the sample or reference. Furthermore, the systems can be capable of detecting the substance along various locations and depths within the sample by mapping a detector pixel and a microoptics to the location and depth in the sample.

A measuring system for determining and/or monitoring the quality of a liquid or viscous foodstuff, including a housing with an interior space for a product container for the foodstuff, a heating and cooling device, and a control unit. The product container has a lid with a thermometer probe projecting into the product container, and a transparent wall. The housing has a light source, an optical sensor for registering light entering the product container via the foodstuff, and a measuring device for rheological properties of the foodstuff. The control unit is connected to the thermometer, the heating and cooling device, the light source, the optical sensor and the measuring device, and is designed to set a predetermined time-temperature program in the interior space, and to repeatedly perform measurements on the foodstuff to determine a measured value of said foodstuff, and for storing and/or exporting and/or processing the measured values.

The present invention is directed to an apparatus (10) for reliable quantitative measurement of a fluorescent blood analyte in tissue (12) comprising: at least one light source (14), the light source (14) emitting excitation light at least at a first wavelength range between 350 nm and 450 nm to the tissue (12); a detection unit (16), the detection unit (16) measuring: a) a portion of the fluorescent light emitted by the fluorescent blood analyte excited at the first wavelength range; and b) a portion of the auto fluorescence emitted by the tissue (12); and/or c1) a portion of the remitted excitation light at the first wavelength range, and c2) a portion of the remitted light at a second wavelength range; and a control unit (18), the control unit (18) operating the light source (14) and detection unit (16). The present invention is further directed to a method for quantitative measurement of a fluorescent blood analyte in tissue (12).

The present invention is directed to an apparatus (10) for reliable quantitative measurement of a fluorescent blood analyte in tissue (12) comprising: at least one light source (14), the light source (14) emitting excitation light at least at a first wavelength range between 350 nm and 450 nm to the tissue (12); a detection unit (16), the detection unit (16) measuring: a) a portion of the fluorescent light emitted by the fluorescent blood analyte excited at the first wavelength range; and b) a portion of the auto fluorescence emitted by the tissue (12); and/or c1) a portion of the remitted excitation light at the first wavelength range, and c2) a portion of the remitted light at a second wavelength range; and a control unit (18), the control unit (18) operating the light source (14) and detection unit (16). The present invention is further directed to a method for quantitative measurement of a fluorescent blood analyte in tissue (12).

Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

A measuring apparatus includes: a light source device that projects light or light of which intensity is periodically modulated onto a measurement object; a light receiver that receives backscattered light of light projected by the light source device from the measurement object; and a processor comprising hardware, the processor being configured to: measure TOF information of the light projected by the light source device and the backscattered light received by the light receiver; acquire distances from a surface of the measurement object to the light source device and the light receiver; and calculate an internal propagation distance in the measurement object according to the measured TOF information and the acquired distances.

A measuring apparatus includes: a light source device that projects light or light of which intensity is periodically modulated onto a measurement object; a light receiver that receives backscattered light of light projected by the light source device from the measurement object; and a processor comprising hardware, the processor being configured to: measure TOF information of the light projected by the light source device and the backscattered light received by the light receiver; acquire distances from a surface of the measurement object to the light source device and the light receiver; and calculate an internal propagation distance in the measurement object according to the measured TOF information and the acquired distances.

An apparatus for generating a two dimensional map representative of a turbid or clear medium (11) includes a system (12) for generating incoherent light within a medium (11), a light collecting system (13) that is movable or stationary relative to the medium (11) being analyzed and that is arranged for collecting light exiting the medium (11), and a spectrum analyzer (14) configured to determine spectrum data of the light exiting the turbid medium (11) and to transmit the spectrum data to a computing unit (15). The computing unit (15) is configured to generate a two dimensional map, in which one dimension of the map is wavelength and a second dimension is a position of the light collecting system (13). The invention is also direct-ed to a method for classifying media using the two dimensional map generated with the apparatus. The method comprises steps of feeding and training neural networks and using the trained neural networks to classify unknown media.