A phase synchronizing pulse amplitude modulation fluorometer having an excitation light source capable of emitting a pulse of light that will induce fluorescence in an external object. A photo detector for detecting the pulse and a circuit to synchronize a pulse of light from the excitation light source with a pulse of light from an external light source.

A method includes identifying a chlorophyll concentration/optical density (CCpOD) value using a chlorophyll concentration measurement of an autotroph culture and an optical density measurement of the autotroph culture. The method also includes identifying a change in the autotroph culture using the CCpOD value. The change in the autotroph culture can be identified by determining whether the CCpOD value falls outside upper and lower control limits. The upper and lower control limits can be identified using a specified number of previously-determined CCpOD values, which can be calculated when the autotroph culture is in a known healthy state. Multiple CCpOD values can be calculated, and an alarm can be generated if a specified number of the CCpOD values (such as one or more) fall outside the upper and lower control limits.

Described and represented is a method for determining the content of at least one plant substance of at least one part of a plant. In order for the content of plant substances, in particular secondary plant substances, of at least one part of a plant to be determined and optimized more expediently, it is provided that the at least one part of the plant is irradiated successively with light of different wavelengths and/or wavelength ranges and that, in response to the irradiation of the at least one part of the plant with light of each wavelength and/or at each wavelength range, the chlorophyll fluorescence of at least substantially the same wavelength and/or at least substantially the same wavelength range is measured in each case.

Methods of deep ultraviolet fluorescence (DUVF) and multi spectral imaging (MSI) detection are disclosed herein for the detection and identification of pathogens on plants. DUV light and visible or near-infrared light are used to illuminate plants or plant leaves such that the light intensity reflected or emitted by the plant or plant leaves can be used to identify the type of pathogen and measure the amount of pathogen on the plant or plant leaves and, additionally, be used to measure the plant's stress response to such pathogen. Also provided herein is a biophotonic analyzer device that uses both DUVF and MSI detection for the monitoring and surveillance of plant health and for the identification and enumeration of pathogens on plants or plant leaves.

One variation of a method includes accessing an image of a plant canopy in an environment inhabited by: a population of microbe sensors of a microbe type including a microbe promoter-reporter pair configured to generate microbe-reporter signals representing presence of a stressor in the environment; and a set of sensor plants of a sensor plant type including a plant promoter-reporter pair configured to signal presence of microbe-reporter signals at the set of sensor plants. The method further includes: accessing a reporter model linking features extracted from images of sensor plants of the sensor plant type to pressures of the set of stressors based on plant-reporter signals generated by the plant promoter-reporter pair and microbe types of microbe sensors inhabiting the environment; and interpreting a pressure of the stressor in the environment based on the reporter model, the microbe type, and features extracted from the image.

A method for detecting at least one property of a plant product, the method including: directing source light including ultraviolet (UV) light at UV wavelengths and polarized visible and/or near-infrared (VIS/NIR) light at VIS/NIR wavelengths onto a region of the plant product; blocking the polarized VIS/NIR light of the source light, and blocking polarized specular reflection from the region of the plant product, from being transmitted to a visible and/or near-infrared (VIS/NIR) spectrometer; and transmitting a portion of emitted light caused by fluorescence and/or diffuse reflection from the region of the plant product to the visible and/or near-infrared (VIS/NIR) spectrometer.

A method for controlling a light intensity in an enclosed cultivation space, the enclosed cultivation space having a light regulating system including an artificial light source for emitting artificial light, the method comprising, detecting a first fluorescence gain and a second fluorescence gain at different light intensity operating points. If the fluorescence gain is greater when the light intensity is greater, then the light intensity setpoint can be increased. Similarly, if the gain is greater when the intensity is lower, then the setpoint can be reduced. The invention is based on the understanding that it is beneficial for plant growth to provide light at a level which corresponds to a maximum ChlF-gain. The light level which corresponds to a maximum ChlF-gain provides a good trade-off between production rate and production efficiency.

The present disclosure relates to an imaging device, an operation method of an imaging device, and a program for enabling visualization and presentation of an invisible imaging target.

Incident light coming from a measurement target is separated. Spectral Raw data is generated on the basis of a spectral result. Spectral reflectance of the measurement target is calculated on the basis of the spectral Raw data. A visualized image is formed on the basis of the spectral reflectance. The visualized image is displayed in real time. The present disclosure is applicable to a spectroscopic camera.

One variation of a method includes accessing an image of a plant canopy in an environment inhabited by: a population of microbe sensors of a microbe type including a microbe promoter-reporter pair configured to generate microbe-reporter signals representing presence of a stressor in the environment; and a set of sensor plants of a sensor plant type including a plant promoter-reporter pair configured to signal presence of microbe-reporter signals at the set of sensor plants. The method further includes: accessing a reporter model linking features extracted from images of sensor plants of the sensor plant type to pressures of the set of stressors based on plant-reporter signals generated by the plant promoter-reporter pair and microbe types of microbe sensors inhabiting the environment; and interpreting a pressure of the stressor in the environment based on the reporter model, the microbe type, and features extracted from the image.

Methods and systems for remote detection of growth conditions of a plant species. In a particular implementation, the present invention provides improved techniques for identifying one or more stress conditions in plants, including without limitation, drought stress, temperature stress, salinity stress and other conditions in a plant species. An example implementation assesses both steady-state chlorophyll fluorescence and green light reflectance of a plant species in real-time. A controller may detect drought and/or other stress conditions by comparing the respective signal trajectories of observed chlorophyll fluorescence and green light reflectance.